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Literature summary for 3.4.21.62 extracted from
- Secretion of an endogenous subtilisin by Pichia pastoris strains GS115 and KM71 (2010), Appl. Environ. Microbiol., 76, 4269-4276.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full-length SUB2 gene from strain GS115 transformed into Escherichia coli. Overexpressed in Pichia pastoris KM71 | Komagataella pastoris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | totally inhibits recombinant Sub2 | Komagataella pastoris |  |
| Aprotinin | inhibits the enzymatic activity by 15% | Komagataella pastoris | |
| chymostatin | totally inhibits recombinant Sub2 | Komagataella pastoris | |
| leupeptin | inhibits the enzymatic activity by 20% | Komagataella pastoris | |
| additional information | is not inhibited by 1,10-phenanthroline, EDTA, E64, bestatin, phosphoramidon and pepstatin A | Komagataella pastoris | |
| phenylmethanesulfonyl fluoride | totally inhibits recombinant Sub2 | Komagataella pastoris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
sequence analysis or mass spectrometry | Komagataella pastoris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Komagataella pastoris | - |
strains GS115 and KM71 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ion-exchange chromatography | Komagataella pastoris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
with N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide as substrate at pH 7.0 | Komagataella pastoris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Komagataella pastoris | ? | - |
? | |
| additional information | Sub2 undergoes self-digestion at high concentrations | Komagataella pastoris | ? | - |
? | |
| N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Komagataella pastoris | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SUB2 | - |
Komagataella pastoris |
| subtilisin | - |
Komagataella pastoris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Komagataella pastoris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | - |
Komagataella pastoris |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | during growth of Pichia pastoris, Sub2 is produced as a secreted enzyme at a concentration of 10 microg/ml of culture supernatant after overexpression of the full-length SUB2 gene. During fermentative production of recombinant enzymes in methanol medium, 1 ml of Pichia pastoris culture supernatant is found to contain approximately 3 ng of Sub2, while the enzyme is not detected during growth in a medium containing glycerol as a carbon source | Komagataella pastoris |
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Release 2023.1 (January 2023)
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