Activating Compound | Comment | Organism | Structure |
---|---|---|---|
choline | increases the enzyme activity | Bacillus licheniformis |
Crystallization (Comment) | Organism |
---|---|
by the batch method from buffer solution saturated with Na2SO4 ca. 13% (w/v) as precipitant. Placement of several Cs+ and Cl- ions in crystals of subtilisin Carlsberg. The protein conformation is very similar to that of the enzyme without CsCl in acetonitrile. 11 defined sites for Cs+ cations and 8 Cl- anions around the protein molecule, although most of these have partial occupancy and may represent nonspecific binding sites. Two Cs+ and two Cl- ions are close to the mouth of the active site cleft, where they may affect catalysis. CsCl-treated subtilisin crystals transferred to acetonitrile show catalytic activity several fold higher than the reference crystals containing Na+ | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cl- | treatment with CsCl increases the activity several fold. Two Cl- ions are close to the mouth of the active site cleft, where they may affect catalysis | Bacillus licheniformis | |
Cs+ | treatment with CsCl increases the activity several fold. Two Cs+ ions are close to the mouth of the active site cleft, where they may affect catalysis | Bacillus licheniformis | |
KCl | increases enzymatic activity somewhat, while choline-Cl has a larger effect | Bacillus licheniformis | |
additional information | enzymatic activity of subtilisin crystals in acetonitrile is sensitive to the type of counterions present before transfer to the organic solvent. Larger cations increase the enzyme activity | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | P00780 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-Tyr ethyl ester + H2O | - |
Bacillus licheniformis | N-acetyl-Tyr + ethanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
subtilisin Carlsberg | - |
Bacillus licheniformis |