Application | Comment | Organism |
---|---|---|
synthesis | synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2 with benzyloxycarbonyl-L-Asp methyl ester as acyl donor and serine amide as the nucleophile. Optimum conditions are pH 10.0, 35°C, in acetonitrile/Na2CO3-NaHCO3 buffer system, 85:15, with a dipeptide yield of 75.5% | Bacillus subtilis |
Organic Solvent | Comment | Organism |
---|---|---|
2-methyl-2-propanol | effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 56.9% at 85% 2-methyl-2-propanol | Bacillus subtilis |
acetonitrile | effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 75.5% at 85% acetonitrile | Bacillus subtilis |
chloroform | effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 51.3% at 85% chloroform | Bacillus subtilis |
dimethylformamide | effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 33.55% at 85% dimethylformamide | Bacillus subtilis |
Ethanol | effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 49.4% at 85% ethanol | Bacillus subtilis |
Ethyl acetate | effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 43.7% at 85% ethyl acetate | Bacillus subtilis |
Methanol | effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 16.3% at 85% methanol | Bacillus subtilis |
Methanol | effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 33.55% at 85% methanol | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
var. Carlsberg, commercial preparation, Novo Industrial, with spec. activity 2.5 AU per ml | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-L-Asp methyl ester + serine amide | - |
Bacillus subtilis | benzyloxycarbonyl-L-Asp-L-Ser-NH2 + methanol | - |
? |