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Literature summary for 3.4.21.62 extracted from

  • Hou, R.Z.; Yang, Y.; Huang, Y.B.; Wang, H.; Zhang, N.; Liu, Y.J.; Zhang, X.Z.; Li, G.
    Alcalase-catalyzed, kinetically controlled synthesis of a precursor dipeptide of RGDS in organic solvents (2006), Prep. Biochem. Biotechnol., 36, 93-105.
    View publication on PubMed

Application

Application Comment Organism
synthesis synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2 with benzyloxycarbonyl-L-Asp methyl ester as acyl donor and serine amide as the nucleophile. Optimum conditions are pH 10.0, 35°C, in acetonitrile/Na2CO3-NaHCO3 buffer system, 85:15, with a dipeptide yield of 75.5% Bacillus subtilis

Organic Solvent Stability

Organic Solvent Comment Organism
2-methyl-2-propanol effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 56.9% at 85% 2-methyl-2-propanol Bacillus subtilis
acetonitrile effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 75.5% at 85% acetonitrile Bacillus subtilis
chloroform effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 51.3% at 85% chloroform Bacillus subtilis
dimethylformamide effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 33.55% at 85% dimethylformamide Bacillus subtilis
Ethanol effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 49.4% at 85% ethanol Bacillus subtilis
Ethyl acetate effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 43.7% at 85% ethyl acetate Bacillus subtilis
Methanol effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 16.3% at 85% methanol Bacillus subtilis
Methanol effect on synthesis of benzyloxycarbonyl-L-Asp-L-Ser-NH2, yield of dipeptide is 33.55% at 85% methanol Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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var. Carlsberg, commercial preparation, Novo Industrial, with spec. activity 2.5 AU per ml
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-L-Asp methyl ester + serine amide
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Bacillus subtilis benzyloxycarbonyl-L-Asp-L-Ser-NH2 + methanol
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