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Literature summary for 3.4.21.62 extracted from

  • Spreer, A.; Ruechel, R.; Reichard, U.
    Characterization of an extracellular subtilisin protease of Rhizopus microsporus and evidence for its expression during invasive rhinoorbital mycosis (2006), Med. Mycol., 44, 723-731.
    View publication on PubMed
Search for more literature for 3.4.21.62

Application

Application Comment Organism
medicine expression during non-fatal human rhinoorbital mucormycosis Rhizopus microsporus var. rhizopodiformis

Inhibitors

Inhibitors Comment Organism Structure
alpha2-Macroglobulin inhibits alkaline proteolytic activity of purified Arp by 80% Rhizopus microsporus var. rhizopodiformis
additional information alkaline proteolytic activity of purified Arp not inhibited by 3,4-dichloroisocumarin, tosyl lysine chloromethyl ketone, tosyl phenylalanine chloromethyl ketone, leupeptin, chymostatin, elastatinal, iodoacetamide, E-64, EDTA, 1,10-phenanthroline, aprotinin, antithrombin III, alpha1-antitrypsin and soybean trypsin inhibitor Rhizopus microsporus var. rhizopodiformis
pepstatin almost completely inhibits acid proteolytic activity, does not inhibit alkaline proteolytic activity of purified Arp Rhizopus microsporus var. rhizopodiformis
phenylmethylsulfonyl fluoride inhibits alkaline proteolytic activity of purified Arp by 100%, does not inhibit acid proteolytic activity Rhizopus microsporus var. rhizopodiformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, SDS-PAGE Rhizopus microsporus var. rhizopodiformis

Organism

Organism UniProt Comment Textmining
Rhizopus microsporus var. rhizopodiformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by ion exchange chromatography and gel filtration. Alkaline proteolytic activity enriched 1480fold with a yield of 27% Rhizopus microsporus var. rhizopodiformis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.47
-
 culture supernatant Rhizopus microsporus var. rhizopodiformis
696
-
 enzyme purified 1480fold Rhizopus microsporus var. rhizopodiformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Rhizopus microsporus var. rhizopodiformis ?
-
 ?
Bovine serum albumin + H2O
-
 Rhizopus microsporus var. rhizopodiformis ?
-
 ?
casein + H2O
-
 Rhizopus microsporus var. rhizopodiformis ?
-
 ?
Elastin + H2O
-
 Rhizopus microsporus var. rhizopodiformis ?
-
 ?
Hemoglobin + H2O
-
 Rhizopus microsporus var. rhizopodiformis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Rhizopus microsporus var. rhizopodiformis

Synonyms

Synonyms Comment Organism
Arp
-
 Rhizopus microsporus var. rhizopodiformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Rhizopus microsporus var. rhizopodiformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 85
-
 Rhizopus microsporus var. rhizopodiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Rhizopus microsporus var. rhizopodiformis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 12
-
 Rhizopus microsporus var. rhizopodiformis

pI Value

Organism Comment pI Value Maximum pI Value
Rhizopus microsporus var. rhizopodiformis determined in granulated dextran gels
-
 8.8