Literature summary for 3.4.21.62 extracted from

Radisky, E.S.; Kwan, G.; Karen Lu, C.J.; Koshland, D.E.
Binding, proteolytic, and crystallographic analyses of mutations at the protease-inhibitor interface of the subtilisin BPN/chymotrypsin inhibitor 2 complex (2004), Biochemistry, 43, 13648-13656.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with chymotrypsin inhibitor 2 and its mutants M59K, M59Y, M59F, M59A, M59G, Y61A	Bacillus amyloliquefaciens

Inhibitors

Inhibitors	Comment	Organism	Structure
chymotrypsin inhibitor 2	-	Bacillus amyloliquefaciens
chymotrypsin inhibitor 2 mutant M59A	-	Bacillus amyloliquefaciens
chymotrypsin inhibitor 2 mutant M59F	-	Bacillus amyloliquefaciens
chymotrypsin inhibitor 2 mutant M59G	-	Bacillus amyloliquefaciens
chymotrypsin inhibitor 2 mutant M59K	-	Bacillus amyloliquefaciens
chymotrypsin inhibitor 2 mutant M59Y	-	Bacillus amyloliquefaciens
chymotrypsin inhibitor 2 mutant Y61A	-	Bacillus amyloliquefaciens

Organism

Organism	UniProt	Comment	Textmining
Bacillus amyloliquefaciens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide + H2O	-	Bacillus amyloliquefaciens	succinyl-L-Ala-L-Ala-L-Pro-Gly + 4-nitroaniline	-	?
succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide + H2O	-	Bacillus amyloliquefaciens	succinyl-L-Ala-L-Ala-L-Pro-L-Ala + 4-nitroaniline	-	?
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide + H2O	-	Bacillus amyloliquefaciens	succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 4-nitroaniline	-	?
succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide + H2O	-	Bacillus amyloliquefaciens	succinyl-L-Ala-L-Ala-L-Pro-L-Met + 4-nitroaniline	-	?
succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O	-	Bacillus amyloliquefaciens	succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline	-	?
succinyl-L-Ala-L-Ala-L-Pro-L-Tyr-4-nitroanilide + H2O	-	Bacillus amyloliquefaciens	succinyl-L-Ala-L-Ala-L-Pro-L-Tyr + 4-nitroaniline	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000000003	-	chymotrypsin inhibitor	-	Bacillus amyloliquefaciens
0.0000000033	-	chymotrypsin inhibitor 2 mutant M59Y	-	Bacillus amyloliquefaciens
0.000000017	-	chymotrypsin inhibitor 2 mutant M59A	-	Bacillus amyloliquefaciens
0.000000017	-	chymotrypsin inhibitor 2 mutant M59F	-	Bacillus amyloliquefaciens
0.000000019	-	chymotrypsin inhibitor 2 mutant M59K	-	Bacillus amyloliquefaciens
0.00000013	-	chymotrypsin inhibitor 2 mutant M59G	-	Bacillus amyloliquefaciens
0.00000046	-	chymotrypsin inhibitor 2 mutant Y61A	-	Bacillus amyloliquefaciens

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Release 2023.1 (January 2023)