Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-bromophenacyl bromide | - |
Bacillus subtilis | |
iodoacetamide | - |
Bacillus subtilis | |
PMSF | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
casein | pH 10, 45°C, Vmax: 0.336 mg/min | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 100% inhibition | Bacillus subtilis | |
Mg2+ | 100% inhibition | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16900 | - |
SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified to 23.5fold by a combination of cation and anion exchange chromatography, ethanol precipitation followed by reverse-phase HPLC | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | - |
Bacillus subtilis | ? | - |
? | |
casein + H2O | - |
Bacillus subtilis | ? | - |
? | |
chicken-feather keratin + H2O | - |
Bacillus subtilis | ? | - |
? | |
Gelatin + H2O | - |
Bacillus subtilis | ? | - |
? | |
Hemoglobin + H2O | - |
Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE, 1 * 16900 Da | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
Alzwiprase | - |
Bacillus subtilis |
subtilisin-like serine protease | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Bacillus subtilis |