Literature summary for 3.4.21.62 extracted from

Tanaka, S.; Matsumura, H.; Koga, Y.; Takano, K.; Kanaya, S.
Four new crystal structures of Tk-subtilisin in unautoprocessed, autoprocessed and mature forms: insight into structural changes during maturation (2007), J. Mol. Biol., 372, 1055-1069.

Search for more literature for 3.4.21.62

Crystallization (Commentary)

Crystallization (Comment)	Organism
by sitting-drop, vapor-diffusion method. Crystal structures of the autoprocessed/unautoprocessed form and mature forms of subtilisin at 1.89 A and 1.70 A resolution, respectively. Crystals show a unique Ca2+-binding site and that the N-terminal region of the mature domain (Gly70Pro82), which binds tightly to the main body in the unautoprocessed form, is disordered and mostly truncated in the autoprocessed and mature forms, respectively. Crystal structure of the propeptide:S324A-subtilisin complex at 1.65 a resolution	Thermococcus kodakarensis

Crystallization (Comment)

Organism

by sitting-drop, vapor-diffusion method. Crystal structures of the autoprocessed/unautoprocessed form and mature forms of subtilisin at 1.89 A and 1.70 A resolution, respectively. Crystals show a unique Ca2+-binding site and that the N-terminal region of the mature domain (Gly70Pro82), which binds tightly to the main body in the unautoprocessed form, is disordered and mostly truncated in the autoprocessed and mature forms, respectively. Crystal structure of the propeptide:S324A-subtilisin complex at 1.65 a resolution

Thermococcus kodakarensis

Protein Variants

Protein Variants	Comment	Organism
S324A	pro-subtilisin, which is not autoprocessed at all when the active site serine residue is replaced with Ala. Is refolded in the presence of Ca2+	Thermococcus kodakarensis
S324C	propeptide:subtilisin complex is stable	Thermococcus kodakarensis

Inhibitors

Inhibitors	Comment	Organism	Structure
diisopropylphosphofluoridate	-	Thermococcus kodakarensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	calcium ion binds weakly to the Ca-7 site in the unautoprocessed form, but is trapped upon autoprocessing. The Ca-7 site is required to promote the autoprocessing reaction by stabilizing the autoprocessed form, in which the new N-terminus of the mature domain is structurally disordered	Thermococcus kodakarensis

Organism

Organism	UniProt	Comment	Textmining
Thermococcus kodakarensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
S324A-subtilisin and propeptide, by gel filtration	Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O	-	Thermococcus kodakarensis	N-succinyl-Ala-Ala-Pro-Phe + p-nitroaniline	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	-	Thermococcus kodakarensis