Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.62 extracted from

  • Choi, N.; Ju, S.; Lee, T.Y.; Yoon, K.; Chang, K.; Maeng, P.J.; Kim, S.
    Miniscale identification and characterization of subtilisins from Bacillus sp. strains (2005), J. Microbiol. Biotechnol., 15, 537-543.
No PubMed abstract available

Application

Application Comment Organism
analysis due to high pI-value of enzyme, it does not migrate in the electrophoretic field in the Laemmli buffer system. Fast and simple identification of enzyme by over-running electrophoretic technique with a miniscale culture supernatant Bacillus subtilis
analysis due to high pI-value of enzyme, it does not migrate in the electrophoretic field in the Laemmli buffer system. Fast and simple identification of enzyme by over-running electrophoretic technique with a miniscale culture supernatant Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: EDTA at 5 mM Bacillus sp. (in: Bacteria)
phenylmethylsulfonyl fluoride
-
Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) DJ-1
-
-
-
Bacillus subtilis
-
-
-

Subunits

Subunits Comment Organism
More N-terminal amino acid sequence Bacillus subtilis
More N-terminal amino acid sequence Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
30 min, stable Bacillus sp. (in: Bacteria)
60
-
30 min, 85% residual activity Bacillus sp. (in: Bacteria)
70
-
30 min, 20% residual activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 stable within this range Bacillus subtilis
3 11 stable within this range Bacillus sp. (in: Bacteria)