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Literature summary for 3.4.21.62 extracted from

  • Chalfoun, N.R.; Grellet-Bournonville, C.F.; Martinez-Zamora, M.G.; Diaz-Perales, A.; Castagnaro, A.P.; Diaz-Ricci, J.C.
    Purification and characterization of AsES protein: a subtilisin secreted by Acremonium strictum is a novel plant defense elicitor (2013), J. Biol. Chem., 288, 14098-14113.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.62

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha Sarocladium strictum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Sarocladium strictum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 x * 34000, SDS-PAGE Sarocladium strictum

Organism

Organism UniProt Comment Textmining
Sarocladium strictum
-
 an avirulent isolate
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from culture filtrate by ultrafiltration, anion exchange and hydrophobic interaction chromatography, to homogeneity Sarocladium strictum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Sarocladium strictum N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
Nalpha-benzoyl-DL-Arg-4-nitroanilide + H2O
-
 Sarocladium strictum Nalpha-benzoyl-DL-Arg + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Sarocladium strictum

Synonyms

Synonyms Comment Organism
AsES
-
 Sarocladium strictum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sarocladium strictum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sarocladium strictum

pI Value

Organism Comment pI Value Maximum pI Value
Sarocladium strictum isoelectric focusing
-
 8.8

General Information

General Information Comment Organism
physiological function fungal subtilisin is a defense elicitor, culture filtrates from avirulent Acremonium spp. induce resistance against pathogens in strawberry plants or accumulation of reactive oxygen species (e.g. H2O2 and O2 -) and callose in Arabidopsis thaliana plants, expression analysis of defense-related genes in strawberry and defense responses induced by the enzyme in Arabidopsis leaves, overview Sarocladium strictum