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Literature summary for 3.4.21.62 extracted from

  • Fasoli, E.; Ferrer, A.; Barletta, G.
    Hydrogen/deuterium exchange study of subtilisin carlsberg during prolonged exposure to organic solvents (2009), Biotechnol. Bioeng., 102, 1025-1032.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information dynamics are also affected by the method of preparation of the enzyme: decreased flexibility is observed when the enzyme is chemically modified with poly ethylene glycol or colyophilized with crown ethers Bacillus licheniformis

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile flexibility of subtilisin Carlsberg decreases during prolonged storage. Is more flexible in acetonitrile than in 1,4-dioxane Bacillus licheniformis
dioxane in 1,4-dioxane, flexibility of subtilisin Carlsberg decreases during prolonged storage: incubation of lyophilized enzyme for a maximum of 96 h (4 days) in 1,4-dioxane, a solvent in which this enzyme is most active and enantioselective, shows a decrease in the rate of exchange of both fast and slow exchanging protons Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
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Synonyms

Synonyms Comment Organism
subtilisin Carlsberg
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Bacillus licheniformis