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4.2.1.2: fumarate hydratase

This is an abbreviated version!
For detailed information about fumarate hydratase, go to the full flat file.

Word Map on EC 4.2.1.2

Reaction

(S)-malate
=
fumarate
 +
H2O

Synonyms

At2g47510, At5g50950, Bxe_A1038, Bxe_A3136, class II fumarase, Fum, FUM C, FUM1, FUM2, FumA, fumarase, fumarase A, fumarase C, fumarase/mesaconase, fumarate hydratase, FumB, FumC, FumD, FumF, FUMR, hydratase, fumarate, L-malate hydro-lyase, LmFH-1, LmFH-2, LmjF24.0320, LmjF29.1960, mesaconase/fumarase, MmcBC, PF1754, PF1755, rv1098c, scFUMC, slFumC, stFUMC, Tneu_1334, Tneu_1335

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.2 fumarate hydratase

Temperature Stability

Temperature Stability on EC 4.2.1.2 - fumarate hydratase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
30 min, complete loss of activity
15 - 70
-
30 min, pH 8.5, activity of FumF is stable with increasing temperature below 58°C in the absence of any stabilizer. When the temperature is higher than 60°C, FumF protein loses almost 50% of its activity
20
-
half-life: 1.6 h, at 0.0438 mg of protein per ml
4
-
half-life: 4.6 h, at 0.0438 mg of protein per ml
44.8
wild-type, 15 min, 50% residual activity
45
purified recombinant enzyme, stable below
47.2
mutant A411V, 15 min, 50% residual activity
49
-
5 min, 50% inhibition, enzyme form FUMA
49.4
mutant A227V, 15 min, 50% residual activity
51
-
5 min, 50% inhibition, enzyme form FUMA
52.4
mutant A227V/A411V, 15 min, 50% residual activity
54.6
mutant A227V/A411V/E175K, 15 min, 50% residual activity
56.3
-
melting temperature
57.7
-
melting temperature
75
-
24 h, 10% loss of activity