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Literature summary for 4.2.1.2 extracted from
- Expression and characterization of fumarase (FUMR) from Rhizopus oryzae (2011), Fungal Biol., 115, 49-53.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fumR, overexpression in Escherichia coli strain BL21 (DE3) | Rhizopus arrhizus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fumarate | the activity of FUMR catalyzing hydration of fumarate to L-malate is completely inhibited by 2 mM fumaric acid | Rhizopus arrhizus |  |
| Mg2+ | slight inhibition | Rhizopus arrhizus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
(S)-malate | pH 7.4, 30°C, recombinant enzyme | Rhizopus arrhizus | |
| 3.07 | - |
fumarate | pH 7.4, 30°C, recombinant enzyme | Rhizopus arrhizus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rhizopus arrhizus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | has a small stimulatory effect | Rhizopus arrhizus | |
| additional information | no effects of Zn2+, Fe2+, or EDTA on enzyme activity | Rhizopus arrhizus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate | Rhizopus arrhizus | - |
fumarate + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus arrhizus | P55250 | gene fumR | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate | - |
Rhizopus arrhizus | fumarate + H2O | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fumarase | - |
Rhizopus arrhizus |
| FUMR | - |
Rhizopus arrhizus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Rhizopus arrhizus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
purified recombinant enzyme, stable below | Rhizopus arrhizus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Rhizopus arrhizus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | purified recombinant enzyme, stable | Rhizopus arrhizus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | fumarase catalyzes the reversible hydration of fumarate to L-malate in Rhizopus oryzae | Rhizopus arrhizus |
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