EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
4.2.1.2 | 4 |
- |
half-life: 4.6 h, at 0.0438 mg of protein per ml |
33751 |
4.2.1.2 | 15 |
70 |
30 min, pH 8.5, activity of FumF is stable with increasing temperature below 58°C in the absence of any stabilizer. When the temperature is higher than 60°C, FumF protein loses almost 50% of its activity |
716122 |
4.2.1.2 | 20 |
- |
half-life: 1.6 h, at 0.0438 mg of protein per ml |
33751 |
4.2.1.2 | 21 |
- |
pH 7.6, stable |
678771 |
4.2.1.2 | 40 |
- |
24 h, stable |
677760 |
4.2.1.2 | 40 |
- |
over 85% of activity after 48 h incubation at 40°C |
730365 |
4.2.1.2 | 44.8 |
- |
wild-type, 15 min, 50% residual activity |
747671 |
4.2.1.2 | 45 |
- |
purified recombinant enzyme, stable below |
715093 |
4.2.1.2 | 47.2 |
- |
mutant A411V, 15 min, 50% residual activity |
747671 |
4.2.1.2 | 49 |
- |
5 min, 50% inhibition, enzyme form FUMA |
33768 |