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Literature summary for 4.2.1.2 extracted from

  • Lin, W.; Chan, M.; Goh, L.L.; Sim, T.S.
    Molecular basis for thermal properties of Streptomyces thermovulgaris fumarase C hinge at hydrophilic amino acids R163, E170 and S347 (2007), Appl. Microbiol. Biotechnol., 75, 329-335.
    View publication on PubMed

Application

Application Comment Organism
synthesis stFUMC is a highly efficient, thermostable fumarase C with industrial potential Streptomyces thermovulgaris

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Streptomyces coelicolor
expression in Escherichia coli BL21 Streptomyces thermovulgaris

Protein Variants

Protein Variants Comment Organism
A347S mutation results in an increase in optimum temperature of 10°C and a fourfold enhancement in specific activity Streptomyces coelicolor
G163R mutation results in an increase in optimum temperature of 5°C Streptomyces coelicolor
G163R/G170E mutant is more thermostable than wild-type scFUMC. Mutation results in an increase in optimum temperature of 5°C Streptomyces coelicolor
G163R/G170E/A347S mutant is more thermostable than wild-type scFUMC. Mutation results in an increase in optimum temperature of 5°C Streptomyces coelicolor
G170E mutation results in an increase in optimum temperature of 5°C Streptomyces coelicolor
G170E/A347S mutation results in an increase in optimum temperature of 5°C Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-
Streptomyces thermovulgaris A5Y6J1
-
-

Synonyms

Synonyms Comment Organism
fumarase C
-
Streptomyces coelicolor
fumarase C
-
Streptomyces thermovulgaris
scFUMC
-
Streptomyces coelicolor
stFUMC
-
Streptomyces thermovulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Streptomyces coelicolor
50
-
-
Streptomyces thermovulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
24 h, stable Streptomyces thermovulgaris
50
-
rapid denaturation at Streptomyces coelicolor