Application | Comment | Organism |
---|---|---|
synthesis | stFUMC is a highly efficient, thermostable fumarase C with industrial potential | Streptomyces thermovulgaris |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Streptomyces coelicolor |
expression in Escherichia coli BL21 | Streptomyces thermovulgaris |
Protein Variants | Comment | Organism |
---|---|---|
A347S | mutation results in an increase in optimum temperature of 10°C and a fourfold enhancement in specific activity | Streptomyces coelicolor |
G163R | mutation results in an increase in optimum temperature of 5°C | Streptomyces coelicolor |
G163R/G170E | mutant is more thermostable than wild-type scFUMC. Mutation results in an increase in optimum temperature of 5°C | Streptomyces coelicolor |
G163R/G170E/A347S | mutant is more thermostable than wild-type scFUMC. Mutation results in an increase in optimum temperature of 5°C | Streptomyces coelicolor |
G170E | mutation results in an increase in optimum temperature of 5°C | Streptomyces coelicolor |
G170E/A347S | mutation results in an increase in optimum temperature of 5°C | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Streptomyces thermovulgaris | A5Y6J1 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
fumarase C | - |
Streptomyces coelicolor |
fumarase C | - |
Streptomyces thermovulgaris |
scFUMC | - |
Streptomyces coelicolor |
stFUMC | - |
Streptomyces thermovulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Streptomyces coelicolor |
50 | - |
- |
Streptomyces thermovulgaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
24 h, stable | Streptomyces thermovulgaris |
50 | - |
rapid denaturation at | Streptomyces coelicolor |