General Stability | Organism |
---|---|
dithiothreitol partially restores from urea and alkaline inactivation | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-malate | mixed-type inhibition | Corynebacterium glutamicum | |
ATP | competitive or mixed-type inhibition | Corynebacterium glutamicum | |
D-Tartrate | competitive | Corynebacterium glutamicum | |
meso-tartrate | competitive | Corynebacterium glutamicum | |
additional information | no inactivation with L-tartrate, citrate, succinate, glycine, maleate, 1 mM iodoacetate, 1 mM iodoacetamide, 1 mM N-ethylmaleimide or 0.5 mM PCMB | Corynebacterium glutamicum | |
pyromellitate | competitive | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
fumarate | MES-NaOH buffer, pH 7.0 | Corynebacterium glutamicum | |
0.67 | - |
fumarate | MES-NaOH buffer, pH 7.0 | Corynebacterium glutamicum | |
0.81 | - |
fumarate | MES-NaOH buffer, pH 6.0 | Corynebacterium glutamicum | |
1.4 | - |
(S)-malate | MES-NaOH buffer, pH 6.0 | Corynebacterium glutamicum | |
1.8 | - |
(S)-malate | MES-NaOH buffer, pH 7.0 | Corynebacterium glutamicum | |
1.8 | - |
(S)-malate | phosphate buffer, pH 6.0 | Corynebacterium glutamicum | |
3.1 | - |
fumarate | phosphate buffer, pH 6.0 | Corynebacterium glutamicum | |
3.8 | - |
fumarate | phosphate buffer, pH 7.0 | Corynebacterium glutamicum | |
4.2 | - |
fumarate | phosphate buffer, pH 6.0 | Corynebacterium glutamicum | |
5 | - |
(S)-malate | MES-NaOH buffer, pH 7.0 | Corynebacterium glutamicum | |
6.3 | - |
(S)-malate | phosphate buffer, pH 7.0 | Corynebacterium glutamicum | |
13 | - |
(S)-malate | phosphate buffer, pH 6.0 | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2 mM, slight stimulation | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, SDS-PAGE | Corynebacterium glutamicum |
200000 | - |
gel filtration | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q8NRN8 | ATCC 14067 | - |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
315 | - |
- |
Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate | - |
Corynebacterium glutamicum | fumarate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, SDS-PAGE | Corynebacterium glutamicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | - |
pH 7.6, stable | Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
(S)-malate | MES-NaOH buffer, pH 6.0 | Corynebacterium glutamicum | |
48 | - |
(S)-malate | phosphate buffer, pH 6.0 | Corynebacterium glutamicum | |
110 | - |
(S)-malate | MES-NaOH buffer, pH 7.0 | Corynebacterium glutamicum | |
200 | - |
(S)-malate | phosphate buffer, pH 7.0 | Corynebacterium glutamicum | |
290 | - |
(S)-malate | MES-NaOH buffer, pH 7.0 | Corynebacterium glutamicum | |
310 | - |
fumarate | MES-NaOH buffer, pH 6.0 | Corynebacterium glutamicum | |
430 | - |
fumarate | MES-NaOH buffer, pH 7.0 | Corynebacterium glutamicum | |
460 | - |
(S)-malate | phosphate buffer, pH 6.0 | Corynebacterium glutamicum | |
510 | - |
fumarate | phosphate buffer, pH 6.0 | Corynebacterium glutamicum | |
650 | - |
fumarate | MES-NaOH buffer, pH 7.0 | Corynebacterium glutamicum | |
720 | - |
fumarate | phosphate buffer, pH 6.0 | Corynebacterium glutamicum | |
730 | - |
fumarate | phosphate buffer, pH 7.0 | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
phosphate buffer, substrate: fumarate | Corynebacterium glutamicum |
7.5 | - |
Tris-HCl buffer, substrate: fumarate | Corynebacterium glutamicum |
8.3 | - |
phosphate buffer, substrate: malate | Corynebacterium glutamicum |
8.5 | - |
Tris-HCl buffer, substrate: malate | Corynebacterium glutamicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
stable | Corynebacterium glutamicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
meso-tartrate | - |
Corynebacterium glutamicum | |
1.3 | - |
pyromellitate | - |
Corynebacterium glutamicum | |
2.5 | - |
(R)-malate | - |
Corynebacterium glutamicum | |
16 | - |
D-Tartrate | - |
Corynebacterium glutamicum |