Application | Comment | Organism |
---|---|---|
synthesis | fumarase is used for the industrial production of L-malate from the substrate fumarate | uncultured marine microorganism |
Cloned (Comment) | Organism |
---|---|
gene fumF, cloned from a plasmid metagenomic library, DNA and amino acid sequence determination and analysis, sequence comparison, and phylogenetic analysis | uncultured marine microorganism |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | inhibits 37% at 5 mM | uncultured marine microorganism | |
EDTA | inhibits 20% at 2.5 mM | uncultured marine microorganism | |
FeCl2 | inhibits 37% at 5 mM | uncultured marine microorganism | |
FeCl3 | inhibits 47.3% at 5 mM | uncultured marine microorganism | |
NH4Cl | inhibits 23% at 5 mM | uncultured marine microorganism | |
SDS | - |
uncultured marine microorganism | |
ZnCl2 | inhibits 55% at 5 mM | uncultured marine microorganism |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, stimulates 10% at 5 mM | uncultured marine microorganism | |
additional information | class II fumarase activity is frequently influenced by divalent metal ions and non-ionic surfactants. No effect by Ca2+ | uncultured marine microorganism |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured marine microorganism | - |
gene fumF | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
726 | - |
purified recombinant FumF, pH 8.5, 55°C, substrate fumarate | uncultured marine microorganism |
Synonyms | Comment | Organism |
---|---|---|
class II fumarase | - |
uncultured marine microorganism |
fumarase | - |
uncultured marine microorganism |
FumF | - |
uncultured marine microorganism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
uncultured marine microorganism |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | 70% of maximum activity below 40°C, and over 70% of maximum activity between 40-60°C, maximal activity at approximately 55°C | uncultured marine microorganism |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 70 | 30 min, pH 8.5, activity of FumF is stable with increasing temperature below 58°C in the absence of any stabilizer. When the temperature is higher than 60°C, FumF protein loses almost 50% of its activity | uncultured marine microorganism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
uncultured marine microorganism |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | pH optimum at pH 8.5, the activity is dramatically suppressed below pH 7.0 and above pH 9.0 | uncultured marine microorganism |
General Information | Comment | Organism |
---|---|---|
metabolism | fumarase catalyzes the reversible hydration of fumarate to L-malate and is a key enzyme in the tricarboxylic acid cycle and in amino acid metabolism | uncultured marine microorganism |