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4.2.1.2: fumarate hydratase

This is an abbreviated version!
For detailed information about fumarate hydratase, go to the full flat file.

Word Map on EC 4.2.1.2

Reaction

(S)-malate
=
fumarate
 +
H2O

Synonyms

At2g47510, At5g50950, Bxe_A1038, Bxe_A3136, class II fumarase, Fum, FUM C, FUM1, FUM2, FumA, fumarase, fumarase A, fumarase C, fumarase/mesaconase, fumarate hydratase, FumB, FumC, FumD, FumF, FUMR, hydratase, fumarate, L-malate hydro-lyase, LmFH-1, LmFH-2, LmjF24.0320, LmjF29.1960, mesaconase/fumarase, MmcBC, PF1754, PF1755, rv1098c, scFUMC, slFumC, stFUMC, Tneu_1334, Tneu_1335

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.2 fumarate hydratase

General Stability

General Stability on EC 4.2.1.2 - fumarate hydratase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
(NH4)2SO4, protects enzyme form FUMA from inactivation at 4°C
-
0.8 M guanidine hydrochloride, 50% loss of activity after 5 min at 75°C, complete inactivation at concentrations above 1.5 M
-
10% glycerol, 0.1 M KCl, 5 mM L-malate or 30% ethyleneglycol, partially protects enzyme form FUMA from inactivation at 4°C
-
50% v/v ethanol, 50% v/v methanol, 50% v/v 2-propanol, 4 M urea or 0.1% SDS, stable after treatment for 10 h at room temperature
-
dithiothreitol partially restores from urea and alkaline inactivation
enzyme form FUMA, oxidation and the concomitant release of iron inactivates the enzyme in a reversible manner
-
enzyme form FUMB is extremely unstable
-
enzyme stability is achieved by addition of soy bean protein or bovine serum albumin.
-
exposure to air at room temperature causes 50% loss of activity, reactivation with FeSO4 and 2-mercaptoethanol
-
exposure to air results in 30% decreased activity
-
mitochondrially targeted fumarase harboring a tobacco etch virus protease recognition sequence is efficiently cleaved by the mitochondrial but not by the cytosolic tobacco etch virus protease. Fumarase is readily cleaved by cytosolic tobacco etch virus when its import into mitochondria is slowed down by either disrupting the activity of the TOMcomplex, lowering the growth temperature, or reducing the inner membrane electrochemical potential
-