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4.2.1.2: fumarate hydratase

This is an abbreviated version!
For detailed information about fumarate hydratase, go to the full flat file.

Word Map on EC 4.2.1.2

Reaction

(S)-malate
=
fumarate
 +
H2O

Synonyms

At2g47510, At5g50950, Bxe_A1038, Bxe_A3136, class II fumarase, Fum, FUM C, FUM1, FUM2, FumA, fumarase, fumarase A, fumarase C, fumarase/mesaconase, fumarate hydratase, FumB, FumC, FumD, FumF, FUMR, hydratase, fumarate, L-malate hydro-lyase, LmFH-1, LmFH-2, LmjF24.0320, LmjF29.1960, mesaconase/fumarase, MmcBC, PF1754, PF1755, rv1098c, scFUMC, slFumC, stFUMC, Tneu_1334, Tneu_1335

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.2 fumarate hydratase

Crystallization

Crystallization on EC 4.2.1.2 - fumarate hydratase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
free enzyme in which both sites are unoccupied by bound ligand, crystallized from a solution of 50 mM MOPS, pH 7.5, 100 mM LiSO4 and 12% PEG 4000, space group I222, X-ray data are collected between 8 and 2.19 A, unit cell parameters: a = 121.6 A, b = 128 A, c = 62.1 A
fumarase C
-
mutant enzyme E315Q
to 2.1 A resolution, space group C222
crystal structures of Mycobacterium tuberculosis Rv1098c in complex with L-malate, fumarate and the competitive inhibitor meso-tartrate is reported
crystal structure solved at 2.4 A resolution. FumC crystallizes as a homodimer containing two subunits of the normally tetrameric enzyme, in which each chain forms an elongated central four-helix bundle capped by two compact domains at the N- and C-termini
fumarase complex with meso-tartaric acid
-
native and recombinant enzyme, native fumarase is crystallized in the presence of the competitive inhibitor meso-tartrate