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3.5.5.1: nitrilase

This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.

Word Map on EC 3.5.5.1

Reaction

A nitrile
+ 2 H2O =
a carboxylate
 +
NH3

Synonyms

3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.1 nitrilase

Purification

Purification on EC 3.5.5.1 - nitrilase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
35% (w/v) ammonium sulfate precipitation
-
ammonium sulfate precipitation and gel filtration
-
ammonium sulfate precipitation and Ni-NTA column chromatography
-
ammonium sulfate precipitation, butyl-Sepharose column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration
-
ammonium sulfate precipitation, Hi-Prep Sephacryl S-200 gel filtration, and Hi-Prep 16/10 Q-Sepharose column chromatography
-
benzonitrilase A, to homogeneity, partial for benzonitrilase B
-
Hi-Prep 16/10 Phenyl FF column chromatography, Q Sepharose column chromatography, Sephacryl S-200 gel filtration, and Superdex 200 gel filtration
-
highly purified, recombinant enzyme
-
Hiprep 16/10 Q XL column chromatography and Sephacryl S-300 gel filtration
HisTrap HP 9 column chromatography
-
ion exchange and hydrophobic and hydrophobic interaction chromatography
near homogeneity, chromatography techniques
-
Ni Sepharose 6 column chromatography
Ni Sepharose column chromatography
Ni-NTA agarose column chromatography and HiLoad 16/60 Superdex 200 gel filtration
-
Ni-NTA column chromatography
Ni-NTA resin chromatography and Superdex 75 gel filtration
-
Ni-Sepharose Fast Flow column chromatography and Sephadex G-15 gel filtration
nitrilase II, to homogeneity, recombinant enzyme
-
partial
phenyl Sepharose column chromatography, Sephacryl S-200 gel filtration, and Q-Sepharose column chromatography
-
Q-Sepharose column chromatography and Sephacryl S-400 gel filtration
-
recombinant enzyme
recombinant enzyme 2fold from Escherichia coli strain BL21-Gold(DE3)/pOK101/pTf16, further purification of the refolded recombinant enzyme after 1 month storage by gel filtration
recombinant enzyme 4fold from Escherichia coli strain BL21-Gold (DE3), co-purifiication with GroEL
recombinant enzyme 9fold from Escherichia coli strain BL21-Gold (DE3) co-purifiication with GroEL, the two proteins are further separated by gel filtration
recombinant His-tagged Nit1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)/pSH by nickel affinity chromatography
to homogeneity
-
to homogeneity, 2step ammonium phosphate precipitation, calcium phosphate gel
-
to homogeneity, 2step chromatography
-
to homogeneity, chromatography techniques
to homogeneity, recombinant enzyme