Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Alcaligenes faecalis |
General Stability | Organism |
---|---|
the nitrilase activity in cross-linked enzyme aggregates is at 30°C and 60°C about 5times more stable than in soluble preparations | Alcaligenes faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.4 | - |
mandelonitrile | soluble recombinant enzyme | Alcaligenes faecalis | |
8.34 | - |
mandelonitrile | recombinant enzyme in cross-linked enzyme aggregates | Alcaligenes faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
SDS-PAGE | Alcaligenes faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
strain DSMZ 30030 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Alcaligenes faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mandelonitrile + 2 H2O | - |
Alcaligenes faecalis | (R)-mandelic acid + NH3 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | soluble recombinant nitrilase has a half-life of 1732.5 min at 30°C and 128.3 min at 50°C | Alcaligenes faecalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
mandelonitrile | soluble recombinant enzyme | Alcaligenes faecalis | |
0.083 | - |
mandelonitrile | recombinant enzyme in cross-linked enzyme aggregates | Alcaligenes faecalis |