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Literature summary for 3.5.5.1 extracted from

  • Kaul, P.; Stolz, A.; Banerjee, U.C.
    Cross-linked amorphous nitrilase aggregates for enantioselective nitrile hydrolysis (2007), Adv. Synth. Catal., 349, 2167-2176.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM109 Alcaligenes faecalis

General Stability

General Stability Organism
the nitrilase activity in cross-linked enzyme aggregates is at 30°C and 60°C about 5times more stable than in soluble preparations Alcaligenes faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4
-
mandelonitrile soluble recombinant enzyme Alcaligenes faecalis
8.34
-
mandelonitrile recombinant enzyme in cross-linked enzyme aggregates Alcaligenes faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
SDS-PAGE Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
strain DSMZ 30030
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mandelonitrile + 2 H2O
-
Alcaligenes faecalis (R)-mandelic acid + NH3
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 soluble recombinant nitrilase has a half-life of 1732.5 min at 30°C and 128.3 min at 50°C Alcaligenes faecalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.072
-
mandelonitrile soluble recombinant enzyme Alcaligenes faecalis
0.083
-
mandelonitrile recombinant enzyme in cross-linked enzyme aggregates Alcaligenes faecalis