Application | Comment | Organism |
---|---|---|
synthesis | synthesis of (R)-mandelic acid from (R,S)-mandelonitrile by use of Escherichia coli overexpressing the enzyme in a bioreactor. After a single batch reaction, (R)-mandelic acid can be obtained with 87% yield and 99.99% enantiomeric excess | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.26 | - |
pH 7.5 | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 (R,S)-mandelonitrile + 4 H2O | - |
Pseudomonas putida | (R)-mandelic acid + NH3 + (S)-mandelonitrile | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas putida |