Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | 20% activation at 1 mM | Rhodococcus rhodochrous |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | nearly complete inhibition at 1 mM | Rhodococcus rhodochrous | |
5,5'-dithiobis(2-nitrobenzoic acid) | complete inhibition at 1 mM | Rhodococcus rhodochrous | |
Ag+ | complete inhibition at 5 mM | Rhodococcus rhodochrous | |
Co2+ | complete inhibition at 1 mM | Rhodococcus rhodochrous | |
Hg2+ | 43% inhibition at 1 mM | Rhodococcus rhodochrous | |
iodoacetic acid | slight inhibition at 1 mM | Rhodococcus rhodochrous | |
o-phenanthroline | complete inhibition at 5 mM | Rhodococcus rhodochrous | |
Zn2+ | complete inhibition at 1 mM | Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Rhodococcus rhodochrous |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylonitrile + H2O | Rhodococcus rhodochrous | - |
acrylic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | Rhodococcus rhodochrous | best substrate | benzoic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Rhodococcus rhodochrous |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | best inducer of enzyme formation: isobutyronitrile | Rhodococcus rhodochrous | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.52 | - |
- |
Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobutyronitrile + H2O | - |
Rhodococcus rhodochrous | 2-aminobutyric acid + NH3 | - |
? | |
2-aminopropionitrile + H2O | low activity | Rhodococcus rhodochrous | 2-aminopropionic acid + NH3 | - |
? | |
2-methylbutyronitrile + H2O | - |
Rhodococcus rhodochrous | 2-methylbutyric acid + NH3 | - |
? | |
acrylonitrile + H2O | - |
Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | best substrate | Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
crotononitrile + H2O | - |
Rhodococcus rhodochrous | NH3 + (z)-but-2-en-1-ol | - |
? | |
DL-alpha-amino-4-methylthio butyronitrile + H2O | - |
Rhodococcus rhodochrous | L-methionine + NH3 | - |
? | |
DL-alpha-amino-n-capronitrile + H2O | - |
Rhodococcus rhodochrous | L-norleucine + NH3 | - |
? | |
DL-alpha-amino-n-valeronitrile + H2O | - |
Rhodococcus rhodochrous | L-norvaline + NH3 | - |
? | |
DL-alpha-aminoisocapronitrile + H2O | - |
Rhodococcus rhodochrous | L-leucine + NH3 | - |
? | |
DL-alpha-aminoisovaleronitrile + H2O | - |
Rhodococcus rhodochrous | L-valine + NH3 | - |
? | |
DL-alpha-aminopropionitrile + H2O | - |
Rhodococcus rhodochrous | D-alanine + NH3 | - |
? | |
lactonitrile + H2O | - |
Rhodococcus rhodochrous | lactate + NH3 | - |
? | |
mandelonitrile + 2 H2O | - |
Rhodococcus rhodochrous | mandelic acid + NH3 | - |
? | |
methacrylonitrile + H2O | low activity | Rhodococcus rhodochrous | methylacrylic acid + NH3 | - |
? | |
n-butyronitrile + H2O | - |
Rhodococcus rhodochrous | n-butyric acid + NH3 | - |
? | |
phenylacetonitrile + 2 H2O | low activity | Rhodococcus rhodochrous | phenylacetate + NH3 | - |
? | |
phenylglycinonitrile + H2O | low actiity | Rhodococcus rhodochrous | phenylglycine + NH3 | - |
? | |
propionitrile + H2O | - |
Rhodococcus rhodochrous | propionic acid + NH3 | - |
? | |
succinonitrile + H2O | low activity | Rhodococcus rhodochrous | succinate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Rhodococcus rhodochrous |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Rhodococcus rhodochrous |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
decreasing activity above | Rhodococcus rhodochrous |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rhodococcus rhodochrous |