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Literature summary for 3.5.5.1 extracted from

  • Zhang, Z.; Xu, J.; He, Y.; Ouyang, L.; Liu, Y.; Imanaka, T.
    Efficient production of (R)-(-)-mandelic acid with highly substrate/product tolerant and enantioselective nitrilase of recombinant Alcaligenes sp. (2010), Process Biochem., 45, 887-891.
No PubMed abstract available
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Alcaligenes sp.

General Stability

General Stability Organism
the recombinant nitrilase (from Escherichia coli strain JM109/pNLE) is quite stable, the residual activity after 10 cycles of reaction is still over 40% of the initial activity Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
21.8
-
 mandelonitrile at 30°C and pH 8.0 Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
 x * 38500, calculated from amino acid sequence Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Alcaligenes sp. C5IIS9
-
-
Alcaligenes sp. ECU0401 C5IIS9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alcaligenes sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.6
-
 purified enzyme, using mandelonitrile as substrate, at 30°C and pH 8.0 Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mandelonitrile + 2 H2O
-
 Alcaligenes sp. (R)-mandelic acid + NH3
-
 ?
mandelonitrile + 2 H2O
-
 Alcaligenes sp. ECU0401 (R)-mandelic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 38500, calculated from amino acid sequence Alcaligenes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Alcaligenes sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60
-
 Alcaligenes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme is quite stable at 30°C, with half-life of 63 h, the enzyme is labile at higher temperatures as revealed by half-lives of 35 h and 22 h at 40°C and 50°C, respectively Alcaligenes sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
175
-
 mandelonitrile at 30°C and pH 8.0 Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Alcaligenes sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 8.6 the nitrilase activity was low at pH less 7.0 or pH above 8.6 Alcaligenes sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8
-
 mandelonitrile at 30°C and pH 8.0 Alcaligenes sp.