3.5.5.1: nitrilase

This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.

Word Map on EC 3.5.5.1

3.5.5.1

enantioselectivity

amidase

hydratase

rhodococcus

biocatalyst

synthesis

rhodochrous

benzonitrile

mandelonitrile

indole-3-acetic

alcaligenes

indole-3-acetonitrile

3-cyanopyridine

dinitriles

phenylacetonitrile

acrylonitrile

bromoxynil

r-mandelic

nhase

acidovorax

ozaenae

facilis

industry

iminodiacetic

gibberella

analysis

Reaction

+ 2 H2O =

Synonyms

3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.5 In nitriles

3.5.5.1 nitrilase

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Results	in table
22	Activating Compound
841	AA Sequence
74	Application
7	CAS Registry Number
54	Cloned(Commentary)
1	Crystallization (Commentary)
23	Disease/ Diagnostics
10	Expression
77	General Information
12	General Stability
241	Inhibitors
31	kcat/KM [mM/s]
3	Ki Value [mM]
148	KM Value [mM]
16	Localization
21	Metals/Ions
104	Molecular Weight [Da]
85	Natural Substrates/ Products (Substrates)
49	Organic Solvent Stability
572	Organism
10	Pathway
24	PDB ID
83	pH Optimum
28	pH Range
20	pH Stability
3	pI Value
5	Posttranslational Modification
147	Protein Variants
60	Purification (Commentary)
7	Reaction
5	Reaction Type
336	Reference
2	Renatured (Commentary)
44	Source Tissue
73	Specific Activity [micromol/min/mg]
3	Storage Stability
1017	Substrates and Products (Substrate)
108	Subunits
79	Synonyms
1	Systematic Name
73	Temperature Optimum [°C]
20	Temperature Range [°C]
59	Temperature Stability [°C]
66	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 3.5.5.1 - nitrilase

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23000

Arthrobacter sp.

gel filtration, benzonitrilase B

260000

strain JM3

30000

310000

gel filtration

32000

32848

x * 32848, calculated from amino acid sequence

729043

33000

Acetivibrio thermocellus

A3DHT2

SDS-PAGE

757454

340000

Bradyrhizobium japonicum

712260

34500

Bradyrhizobium japonicum

10 * 34500

712260

35790

Aeribacillus pallidus

Q0PIV8

16 * 35790, calculated from amino acid sequence

710981

35940

Fusarium proliferatum

x * 35940, calculated from amino acid sequence

735075

36500

Rhodococcus rhodochrous

2 * 36500, inactive enzyme, reducing SDS-PAGE

36700

37000

376000

recombinant enzyme, gel filtration

38000

38500

38560

predicted protein size

684594

38830

uncultured Burkholderiaceae bacterium

A0A140D8I8

calculated from sequence

756289

40000

11 entries

41000

6 entries

410000

Rhodococcus rhodochrous

strain J1

651610

412000

Rhodococcus rhodochrous

sedimentation equilibrium analysis, after recovery conditions for hydrolysis of acrylonitrile

41500

1 * 41500, SDS-PAGE

42000

420000

42700

x * 42700, recombinant enzyme, SDS-PAGE, x * 38500, native enzyme, SDS-PAGE, the multimeric nitrilase is composed of 12-16 subunits, mass spectrometry, analytical centrifugation, and dynamic light scattering, modelling

719168

43000

2 entries

44000

Alcaligenes faecalis

x * 32000, strain ATCC 8750, x * 44000, strain JM3

440000

gel filtration

45000

450000

45800

12 * 45800

46000

gel filtration, aggregation to 560 kDa in the presence of benzonitrile

209754

460000

Alcaligenes faecalis

strain ATCC 8750

651610

480000

Rhodococcus rhodochrous

gel filtration

686652

520000

Paenarthrobacter aurescens

S5M6X9

gel filtration

734397, 757343

55000

Rhodococcus erythropolis

x * 55000, SDS-PAGE

550000

560000

580000

60000

600000

620000

650000

72000

Aeribacillus pallidus

x * 41000 + x * 72000, enzyme + groEL

651610

74000

Klebsiella pneumoniae

651610

76000

3 entries

80000

Rhodococcus rhodochrous

gel filtration, only active on benzonitrile

209743