Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition at 0.25 mM, also inhibition of enzyme association to decamers | Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE, no hydrolysis of acrylonitrile | Rhodococcus rhodochrous |
40000 | - |
10 * 40000, SDS-PAGE, hydrolysis of both benzonitrile and acrylonitrile after recovery conditions for acrylonitrile hydrolysis | Rhodococcus rhodochrous |
80000 | - |
gel filtration, only active on benzonitrile | Rhodococcus rhodochrous |
412000 | - |
sedimentation equilibrium analysis, after recovery conditions for hydrolysis of acrylonitrile | Rhodococcus rhodochrous |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + 2 H2O | Rhodococcus rhodochrous | lower activity for the purified enzyme compared to the activity in resting cells | nicotinic acid + NH3 | - |
? | |
acrylonitrile + H2O | Rhodococcus rhodochrous | only hydrolysed after recovered activity by preincubation with benzonitrile or dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate | acrylic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | Rhodococcus rhodochrous | best substrate | benzoic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, 2step chromatography | Rhodococcus rhodochrous |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | induction of enzyme synthesis by epsilon-caprolactam- or isovaleronitrile-containing media | Rhodococcus rhodochrous | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + 2 H2O | lower activity for the purified enzyme compared to the activity in resting cells | Rhodococcus rhodochrous | nicotinic acid + NH3 | - |
? | |
acrylonitrile + H2O | only hydrolysed after recovered activity by preincubation with benzonitrile or dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate | Rhodococcus rhodochrous | acrylic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | best substrate | Rhodococcus rhodochrous | benzoic acid + NH3 | - |
? | |
crotononitrile + H2O | low activity | Rhodococcus rhodochrous | NH3 + (z)-but-2-en-1-ol | - |
? | |
methacrylonitrile + H2O | very low activity in resting cells and for the purified enzyme only after recovered activity by dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate | Rhodococcus rhodochrous | methylacrylic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 40000, SDS-PAGE, hydrolysis of both benzonitrile and acrylonitrile after recovery conditions for acrylonitrile hydrolysis | Rhodococcus rhodochrous |
dimer | 2 * 40000, SDS-PAGE, no hydrolysis of acrylonitrile | Rhodococcus rhodochrous |