3.5.5.1: nitrilase
This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.
Word Map on EC 3.5.5.1
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3.5.5.1
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enantioselectivity
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amidase
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hydratase
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rhodococcus
-
biocatalyst
-
synthesis
-
rhodochrous
-
benzonitrile
-
mandelonitrile
-
indole-3-acetic
-
alcaligenes
-
indole-3-acetonitrile
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3-cyanopyridine
-
dinitriles
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phenylacetonitrile
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acrylonitrile
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bromoxynil
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r-mandelic
-
nhase
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acidovorax
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ozaenae
-
facilis
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industry
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iminodiacetic
-
gibberella
-
analysis
- 3.5.5.1
-
enantioselectivity
- amidase
-
hydratase
- rhodococcus
-
biocatalyst
- synthesis
- rhodochrous
- benzonitrile
- mandelonitrile
-
indole-3-acetic
- alcaligenes
- indole-3-acetonitrile
- 3-cyanopyridine
-
dinitriles
- phenylacetonitrile
- acrylonitrile
- bromoxynil
-
r-mandelic
- nhase
-
acidovorax
- ozaenae
- facilis
- industry
-
iminodiacetic
- gibberella
- analysis
Reaction
Synonyms
3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122
ECTree
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Natural Substrates Products
Natural Substrates Products on EC 3.5.5.1 - nitrilase
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REACTION DIAGRAM
2,4-dichlorophenyl acetonitrile + 2 H2O
2,4-dichlorophenyl acetic acid + NH3
-
-
-
-
?
2-amino-4-methylbenzonitrile + 2 H2O
2-amino-4-methylbenzoic acid + NH3
-
-
-
-
?
2-amino-5-chloro-benzonitrile + 2 H2O
2-amino-5-chloro-benzoic acid + NH3
-
-
-
-
?
2-amino-6-chloro-benzonitrile + 2 H2O
2-amino-6-chloro-benzoic acid + NH3
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good substrate, when the cells are induced by benzonitrile
-
-
?
3,4,5-trimethoxybenzonitrile + 2 H2O
3,4,5-trimethoxybenzoic acid + NH3
-
-
-
-
?
4,5-dimethoxybenzonitrile + 2 H2O
4,5-dimethoxybenzoic acid + NH3
-
-
-
-
?
4-methoxy-2-nitrobenzonitrile + 2 H2O
4-methoxy-2-nitrobenzoic acid + NH3
-
-
-
-
?
acrylonitrile + 2 H2O
acrylic acid + NH3
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preferred substrate, acrylonitrile is also a good inducer of enzyme production
-
-
?
bromoxynil + H2O
?
-
enzyme utilizes the herbicide substrate as nitrogen source
-
-
?
indole-3-acetonitrile + H2O
indole-3-acetic acid + NH3
-
isozymes are involved in indole-3-acetic acid production
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
-
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
-
-
?
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
-
-
-
?
2-methyl-2-phenylpropionic acid + NH3
-
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
-
-
-
?
2-methyl-2-phenylpropionitrile + 2 H2O
2-methyl-2-phenylpropionic acid + NH3
-
-
-
-
?
3-cyanopyridine + 2 H2O
nicotinic acid + NH3
-
100% activity, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine
-
-
?
3-cyanopyridine + 2 H2O
nicotinic acid + NH3
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lower activity for the purified enzyme compared to the activity in resting cells
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
-
-
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
-
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
-
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
69.25% activity compared to 3-cyanopyridine, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
-
-
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
best substrate, no formation of amide
-
?
acrylonitrile + H2O
acrylic acid + NH3
-
only hydrolysed after recovered activity by preincubation with benzonitrile or dialysis against buffer containing 50%, v/v, glycerol and 10% saturated ammonium sulfate
-
?
benzoate + NH3
preferred substrate
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
preferred substrate, when the cells are induced by benzonitrile
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
preferred substrate, when the cells are induced by benzonitrile
-
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
only traces of activity
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
specific for nitrile groups directly attached to the benzene ring
-
?
benzonitrile + 2 H2O
benzoic acid + NH3
-
specific for nitrile groups directly attached to the benzene ring
-
?
indol-3-acetonitrile + H2O
indol-3-acetic acid + NH3
-
-
-
?
phenylacetonitrile + 2 H2O
phenylacetate + NH3
-
-
-
?
?
-
-
the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. Substrate specificity in vivo is dependent on the inducer component, overview
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-
?
additional information
?
-
-
the enzyme hydrolyzes aliphatic, heterocyclic and aromatic nitriles with different substitutions, and is efficient in conversion of both aliphatic and aromatic nitriles. Substrate specificity in vivo is dependent on the inducer component, overview
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-
?
additional information
?
-
isozyme NIT2 might be involved in supplying indole-3-acetic acid during seed development rather than during stratification
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-
?
additional information
?
-
isozyme NIT2 might be involved in supplying indole-3-acetic acid during seed development rather than during stratification
-
-
?
additional information
?
-
isozyme NIT2 might be involved in supplying indole-3-acetic acid during seed development rather than during stratification
-
-
?
additional information
?
-
-
isozyme NIT2 might be involved in supplying indole-3-acetic acid during seed development rather than during stratification
-
-
?