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3.5.5.1: nitrilase

This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.

Word Map on EC 3.5.5.1

Reaction

A nitrile
+ 2 H2O =
a carboxylate
 +
NH3

Synonyms

3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.1 nitrilase

Temperature Stability

Temperature Stability on EC 3.5.5.1 - nitrilase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-70
-
after five freeze-thaw cycles and subsequent storage for 5 days at -70°C, the activity of the enzyme decreases to about one-fifth of the original activity
0
-
half-life: 10 h
20 - 60
the yield of product decreases sharply after the enzyme is heat-treated at 40°C for 30 min and 1 h. The enzyme completely loses its activity after heat-treatment at 60°C for 30 min. However, this enzyme is relatively stable from 20 to 30°C
25 - 35
recombinant enzyme in cell-free extract, pH 8.0, 1 h, stable
25 - 40
purified recombinant enzyme, pH 8.0, 1 h, stable
30 - 50
30 - 55
-
stable at 30°C, highly active at 48°C (with 92% of maximum activity), but its activity declines sharply at higher temperatures (to 53% and 19% at 50°C and 55°C, respectively), at 30°C and pH 7.2-9.0 the enzyme half-life is about 11 h, at 35°C and 40°C, the half-life decreases to 6.2 h and 2.8 h, respectively
35 - 55
-
the nitrilase half-life is 277 min and 10.5 h at 35°C and 45°C, respectively, the enzyme is active at up to 50°C, the enzyme shows only traces of activity at 55°C
37
-
purified enzyme, half-life is 21 h
4
-
activity of cross-linked enzyme aggregate preparation remains stable up to 75 h at 4°C while the free enzyme loses 90% activity at this temperature in 75 h
46.8
-
the melting temperature of the enzyme is 46.8°C
49.3
-
the melting temperature of the enzyme is 49.3°C
65
1 min, about 40% loss of activity
75
1 min, about 70% loss of activity
80
1 min, about 95% loss of activity
85
-
8 h, the enzyme retains more than 50% of its activity
90
-
half-life: 8 h