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Literature summary for 3.5.5.1 extracted from
- A new nitrilase-producing strain named Rhodobacter sphaeroides LHS-305: biocatalytic characterization and substrate specificity (2011), Appl. Biochem. Biotechnol., 165, 1556-1567.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | nitrilases are useful biocatalysts for the hydrolysis of nitriles in a mild and environmentally friendly manner assuring a clean process and specificity with high yield | Cereibacter sphaeroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Cyanopyridine | inhibitory by substrate inhibition above 400 mM | Cereibacter sphaeroides |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, overview | Cereibacter sphaeroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | - |
- |
- |
| Cereibacter sphaeroides LHS-305 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | optimization of culture conditions for nitrilase production, overview. Fe2+ is the best metal ion, pH 7.0 and 28°Care the best conditions, phenylacetamide is the best inducer | Cereibacter sphaeroides | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.075 | - |
cell extract, pH 9.0, 30°C | Cereibacter sphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-cyanopyridine + 2 H2O | - |
Cereibacter sphaeroides | nicotinic acid + NH3 | - |
? | |
| 3-cyanopyridine + 2 H2O | - |
Cereibacter sphaeroides LHS-305 | nicotinic acid + NH3 | - |
? | |
| acrylonitrile + 2 H2O | - |
Cereibacter sphaeroides | acrylic acid + NH3 | - |
? | |
| acrylonitrile + 2 H2O | - |
Cereibacter sphaeroides LHS-305 | acrylic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Cereibacter sphaeroides | benzoic acid + NH3 | - |
? | |
| benzonitrile + 2 H2O | - |
Cereibacter sphaeroides LHS-305 | benzoic acid + NH3 | - |
? | |
| additional information | substrate specificity, overview. No activity with phenylacetonitrile, 4-methoxy-phenylacetonitrile, mandelonitrile, 2-amino propionitrile, and butyronitrile | Cereibacter sphaeroides | ? | - |
? | |
| additional information | substrate specificity, overview. No activity with phenylacetonitrile, 4-methoxy-phenylacetonitrile, mandelonitrile, 2-amino propionitrile, and butyronitrile | Cereibacter sphaeroides LHS-305 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Cereibacter sphaeroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
purified enzyme, half-life is 25 h | Cereibacter sphaeroides |
| 37 | - |
purified enzyme, half-life is 21 h | Cereibacter sphaeroides |
| 50 | - |
purified enzyme, half-life is 8 h | Cereibacter sphaeroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
best in Na2HPO4/KH2PO4 buffer compared to Na2CO3/NaHCO3 buffer | Cereibacter sphaeroides |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Cereibacter sphaeroides | downregulation in cell culture by addition of 3-cyanopyridine, nicotinic acid, urea, phenylacetic acid, phenylacetonitrile, benzoic acid, acrylic acid, and slightly by nicotinamide | down |
| Cereibacter sphaeroides | upregulation in cell culture by addition of acetonitrile, phenylacetamide, and benzonitrile | up |
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Release 2023.1 (January 2023)
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