3.5.5.1: nitrilase
This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.
Word Map on EC 3.5.5.1
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3.5.5.1
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enantioselectivity
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amidase
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hydratase
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rhodococcus
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biocatalyst
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synthesis
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rhodochrous
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benzonitrile
-
mandelonitrile
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indole-3-acetic
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alcaligenes
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indole-3-acetonitrile
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3-cyanopyridine
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dinitriles
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phenylacetonitrile
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acrylonitrile
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bromoxynil
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r-mandelic
-
nhase
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acidovorax
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ozaenae
-
facilis
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industry
-
iminodiacetic
-
gibberella
-
analysis
- 3.5.5.1
-
enantioselectivity
- amidase
-
hydratase
- rhodococcus
-
biocatalyst
- synthesis
- rhodochrous
- benzonitrile
- mandelonitrile
-
indole-3-acetic
- alcaligenes
- indole-3-acetonitrile
- 3-cyanopyridine
-
dinitriles
- phenylacetonitrile
- acrylonitrile
- bromoxynil
-
r-mandelic
- nhase
-
acidovorax
- ozaenae
- facilis
- industry
-
iminodiacetic
- gibberella
- analysis
Reaction
Synonyms
3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122
ECTree
3.5.5.1 nitrilaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.5.5.1 - nitrilase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
21.8
(R,S)-2-hydroxy-2-phenylacetonitrile
pH 8.0, 40°C, recombinant enzyme
0.23
3-Phenylpropionitrile
isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C
2.426
4-methylbenzonitrile
-
wild type enzyme, in 50 mM potassium phosphate (pH 7.5), at 25°C
0.34
4-phenylbutyronitrile
isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C
0.0000000097
Cinnamonitrile
pH 7.4, 70°C, mutant enzyme K96R
0.35
heptanenitrile
isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C
0.88
(methylthio)acetonitrile
pH 8.0, 30°C, isozyme NIT1
1.09
(methylthio)acetonitrile
pH 8.0, 30°C, isozyme NIT2
1.31
(methylthio)acetonitrile
pH 8.0, 30°C, isozyme NIT3
280
1-cyanocyclohexaneacetonitrile
40°C, pH 7.0, enzyme ecapsulated in ethyleneamine-mediated biosilica
0.13
3-(4-chlorophenyl)pentanedinitrile
mutant F202V, sodium phosphate buffer, pH 7.0, 30°C
0.25
3-(4-chlorophenyl)pentanedinitrile
wild-type enzyme, sodium phosphate buffer, pH 7.0, 30°C
0.31
3-(4-chlorophenyl)pentanedinitrile
mutant P194A/F202V, sodium phosphate buffer, pH 7.0, 30°C
0.38
3-(4-chlorophenyl)pentanedinitrile
mutant P194A, sodium phosphate buffer, pH 7.0, 30°C
0.44
3-(4-chlorophenyl)pentanedinitrile
mutant P194A/I201A/F202V, sodium phosphate buffer, pH 7.0, 30°C
0.456
3-aminobenzonitrile
-
mutant enzyme R129K, in 50 mM potassium phosphate (pH 7.5), at 25°C
0.483
3-aminobenzonitrile
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mutant enzyme R129H, in 50 mM potassium phosphate (pH 7.5), at 25°C
1.863
3-aminobenzonitrile
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wild type enzyme, in 50 mM potassium phosphate (pH 7.5), at 25°C
0.000000103
3-anilinoproprionitrile
pH 7.4, 70°C, mutant enzyme K96R
0.000000157
3-anilinoproprionitrile
pH 7.4, 70°C, wild-type enzyme
0.55
3-methylbenzonitrile
-
mutant enzyme R129K, in 50 mM potassium phosphate (pH 7.5), at 25°C
0.592
3-methylbenzonitrile
-
mutant enzyme R129H, in 50 mM potassium phosphate (pH 7.5), at 25°C
1.851
3-methylbenzonitrile
-
wild type enzyme, in 50 mM potassium phosphate (pH 7.5), at 25°C
1.699
4-aminobenzonitrile
-
wild type enzyme, in 50 mM potassium phosphate (pH 7.5), at 25°C
2.49
allyl cyanide
isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C
1.068
Benzonitrile
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wild type enzyme, in 50 mM potassium phosphate (pH 7.5), at 25°C
1.468
Benzonitrile
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mutant enzyme R129K, in 50 mM potassium phosphate (pH 7.5), at 25°C
3.13
Benzonitrile
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mutant enzyme R129H, in 50 mM potassium phosphate (pH 7.5), at 25°C
536.2
iminodiacetonitrile
35°C, pH 7.5, mutant enzyme F168V/T201N/S192F/M191T/F192S
2.12
indole-3-acetonitrile
isozyme NIT-T2, in 50 mM sodium phosphate (pH 8.0) containing 0.2% (w/v) BSA with 5 mM substrate, at 35°C
7.4
indole-3-acetonitrile
pH 8.0, 30°C, isozyme NIT2
11.1
indole-3-acetonitrile
pH 8.0, 30°C, isozyme NIT1
30.1
indole-3-acetonitrile
pH 8.0, 30°C, isozyme NIT3
8.34
mandelonitrile
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recombinant enzyme in cross-linked enzyme aggregates
0.14
phenylacetonitrile
pH 8.0, 30°C, isozyme NIT1
0.23
phenylacetonitrile
pH 8.0, 30°C, isozyme NIT3
0.07
phenylbutyronitrile
pH 8.0, 30°C, isozyme NIT3
0.08
phenylbutyronitrile
pH 8.0, 30°C, isozyme NIT1
0.09
phenylbutyronitrile
pH 8.0, 30°C, isozyme NIT2
0.000000009
phenylglycinenitrile
pH 7.4, 70°C, mutant enzyme K96R
0.000000024
phenylglycinenitrile
pH 7.4, 70°C, wild-type enzyme
0.043
phenylpropionitrile
-
pH and temperature not specified in the publication
0.22
phenylpropionitrile
pH 8.0, 30°C, isozyme NIT1
0.42
phenylpropionitrile
pH 8.0, 30°C, isozyme NIT3
0.43
phenylpropionitrile
pH 8.0, 30°C, isozyme NIT2
0.0000000005
trichloroacetonitrile
pH 7.4, 70°C, wild-type enzyme
0.00000013
trichloroacetonitrile
pH 7.4, 70°C, mutant enzyme K96R
additional information
additional information
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kinetic and chiral analysis, overview
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additional information
additional information
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kinetic and chiral analysis, overview
-
additional information
additional information
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kinetic and chiral analysis, overview
-
additional information
additional information
kinetic and chiral analysis, overview
-
additional information
additional information
-
kinetic and chiral analysis, overview
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