3.5.5.1: nitrilase

This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.

Reaction

Synonyms

3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.5 In nitriles

                3.5.5.1 nitrilase

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Results	in table
22	Activating Compound
841	AA Sequence
74	Application
7	CAS Registry Number
54	Cloned(Commentary)
1	Crystallization (Commentary)
23	Disease/ Diagnostics
10	Expression
77	General Information
12	General Stability
241	Inhibitors
31	kcat/KM [mM/s]
3	Ki Value [mM]
148	KM Value [mM]
16	Localization
21	Metals/Ions
104	Molecular Weight [Da]
85	Natural Substrates/ Products (Substrates)
49	Organic Solvent Stability
572	Organism
10	Pathway
24	PDB ID
83	pH Optimum
28	pH Range
20	pH Stability
3	pI Value
5	Posttranslational Modification
147	Protein Variants
60	Purification (Commentary)
7	Reaction
5	Reaction Type
336	Reference
2	Renatured (Commentary)
44	Source Tissue
73	Specific Activity [micromol/min/mg]
3	Storage Stability
1017	Substrates and Products (Substrate)
108	Subunits
79	Synonyms
1	Systematic Name
73	Temperature Optimum [°C]
20	Temperature Range [°C]
59	Temperature Stability [°C]
66	Turnover Number [1/s]

Organic Solvent Stability

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Organic Solvent Stability on EC 3.5.5.1 - nitrilase

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ORGANIC SOLVENT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2-propanol

Fusarium solani

-

the enzyme shows more than 30% of the control activity with 5% 2-propanol

688441

Acetone

6 entries

acetonitrile

4 entries

dichloromethane

Fusarium solani

-

the enzyme shows more than 30% of the control activity with 5% dichloromethane

688441

dimethyl acetamide

Alcaligenes faecalis

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced at 5%

699360

dimethyl formamide

Alcaligenes faecalis

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-25%

699360

dimethyl sulfoxide

Alcaligenes faecalis

-

signifantly enhances the synthetic potential of the enzyme as well as its enantioselectivity. Extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5–25%

699360

dimethylformamide

2 entries

dimethylsulfoxide

Fusarium solani

-

the enzyme shows more than 30% of the control activity with 5-10% of dimethylsulfoxide

688441

dioxane

Alcaligenes faecalis

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-10%

699360

DMSO

Hoeflea phototrophica

-

5% (v/v), about 10% loss of activity

755874

Ethanol

6 entries

Ethyl acetate

2 entries

isopropanol

Alcaligenes faecalis

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-15%

699360

Methanol

6 entries

n-heptane

Fusarium solani

-

more than half of activity is preserved at up to 50% of n-heptane

688441

n-hexane

Fusarium solani

-

more than half of activity is preserved at up to 50% of n-hexane

688441

N-methyl pyrrolidone

Alcaligenes faecalis

-

addition results in decreased rate of hydrolysis

699360

n-propanol

Alcaligenes faecalis

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-15%

699360

N-vinyl pyrrolidone

Alcaligenes faecalis

-

addition results in decreased rate of hydrolysis

699360

Pyridine

2 entries

tetrahydrofuran

Alcaligenes faecalis

-

addition results in decreased rate of hydrolysis

699360

toluene

Fusarium solani

-

the enzyme shows more than 30% of the control activity with 5-10% of toluene

688441

xylene

Fusarium solani

-

more than half of activity is preserved at up to 15% of xylene, the enzyme shows more than 30% of the control activity with 5-10% of xylene

688441

additional information

4 entries

Acetone

Alcaligenes faecalis

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-15%

699360

Acetone

Alcaligenes faecalis MTCC 126

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-15%

-

Acetone

Fusarium solani

-

the enzyme shows more than 30% of the control activity with 5% acetone

688441

Acetone

Fusarium solani O1

-

the enzyme shows more than 30% of the control activity with 5% acetone

-

Acetone

Hoeflea phototrophica

-

5% (v/v), about 60% loss of activity

755874

Acetone

Hoeflea phototrophica DFL-43

-

5% (v/v), about 60% loss of activity

-

acetonitrile

Fusarium solani

-

the enzyme shows more than 30% of the control activity with 5% acetonitrile

688441

acetonitrile

Fusarium solani O1

-

the enzyme shows more than 30% of the control activity with 5% acetonitrile

-

acetonitrile

Hoeflea phototrophica

-

5% (v/v), no loss of activity

755874

acetonitrile

Hoeflea phototrophica DFL-43

-

5% (v/v), no loss of activity

-

dimethylformamide

Hoeflea phototrophica

-

5% (v/v), about 15% loss of activity

755874

dimethylformamide

Hoeflea phototrophica DFL-43

-

5% (v/v), about 15% loss of activity

-

Ethanol

Alcaligenes faecalis

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-20%

699360

Ethanol

Alcaligenes faecalis MTCC 126

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-20%

-

Ethanol

Fusarium solani

-

more than half of activity is preserved at up to 15% of ethanol

688441

Ethanol

Fusarium solani O1

-

more than half of activity is preserved at up to 15% of ethanol

-

Ethanol

Hoeflea phototrophica

-

5% (v/v), about 15% loss of activity

755874

Ethanol

Hoeflea phototrophica DFL-43

-

5% (v/v), about 15% loss of activity

-

Ethyl acetate

Fusarium solani

-

the enzyme shows more than 30% of the control activity with 5% ethyl acetate

688441

Ethyl acetate

Fusarium solani O1

-

the enzyme shows more than 30% of the control activity with 5% ethyl acetate

-

Methanol

Alcaligenes faecalis

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-20%

699360

Methanol

Alcaligenes faecalis MTCC 126

-

extent of nitrile hydrolysis and enantiomeric purity of product are significantly enhanced in the range of 5-20%

-

Methanol

Fusarium solani

-

the enzyme shows more than 30% of the control activity with 5-10% of methanol

688441

Methanol

Fusarium solani O1

-

the enzyme shows more than 30% of the control activity with 5-10% of methanol

-

Methanol

Hoeflea phototrophica

-

5% (v/v), no loss of activity

755874

Methanol

Hoeflea phototrophica DFL-43

-

5% (v/v), no loss of activity

-

Pyridine

Alcaligenes faecalis

-

addition results in decreased rate of hydrolysis

699360

Pyridine

Alcaligenes faecalis MTCC 126

-

addition results in decreased rate of hydrolysis

-

additional information

Alcaligenes faecalis

-

addition of organic solvent up to 10%-20% leads to an enhancement in reaction rate, any further increase beyond the critical concentration in the latter leads to the decrease in catalytic efficiency of the enzyme. The solvent dielectric constant shows a linear correlation with the critical concentration of the solvent used and the extent of nitrile hydrolysis. Unlike alcohols, the reaction rate in case of aprotic solvents can be linearly correlated to solvent log P. The affinity of the enzyme for its substrate is highly dependent upon the aprotic solvent used

699360

additional information

Alcaligenes faecalis MTCC 126

-

addition of organic solvent up to 10%-20% leads to an enhancement in reaction rate, any further increase beyond the critical concentration in the latter leads to the decrease in catalytic efficiency of the enzyme. The solvent dielectric constant shows a linear correlation with the critical concentration of the solvent used and the extent of nitrile hydrolysis. Unlike alcohols, the reaction rate in case of aprotic solvents can be linearly correlated to solvent log P. The affinity of the enzyme for its substrate is highly dependent upon the aprotic solvent used

-

additional information

Fusarium proliferatum

-

when 20% (v/v) of organic solvent is used, the enzyme activity sharply decreases and only can be detected with very low activity except that in methanol and 2-chloromethane (40% and 10% of activity retaining, respectively)

735075

additional information

Fusarium proliferatum CA3-1

-

when 20% (v/v) of organic solvent is used, the enzyme activity sharply decreases and only can be detected with very low activity except that in methanol and 2-chloromethane (40% and 10% of activity retaining, respectively)

-