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3.5.5.1: nitrilase

This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.

Word Map on EC 3.5.5.1

Reaction

A nitrile
+ 2 H2O =
a carboxylate
 +
NH3

Synonyms

3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.1 nitrilase

pH Stability

pH Stability on EC 3.5.5.1 - nitrilase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11.5
purified recombinant enzyme, room temperature, 2 h, no activity remaining
718670
4
-
24 h, complete loss of activity
758526
4 - 7.5
the wild type enzyme shows no activity after 30 min incubation at pH 4.5, about 10% activity after 30 min incubation at pH 5.0, about 20% activity after 30 min incubation at pH 5.5, about 60% activity after 30 min incubation at pH 6.0, and 100% activity after 30 min incubation at pH 6.5-7.5
711870
4.5 - 8
-
24 h, less than 20% loss of activity
758526
5 - 11
-
the enzyme shows 5%, 39% and 29% of the maximum activity at pH 5, 6 and 10, respectively, and it is nearly inactive at pH 11
688441
5.5
purified recombinant enzyme, room temperature, 2 h, 60% activity remaining
718670
5.5 - 10
-
a sharp activity decrease is observed at slightly acidic pH values, only a trace activity being observed at pH 5.5, 70% and 17% of the activity being retained at pH 9 and 10, respectively
684594
6
purified recombinant enzyme, room temperature, 2 h, 80% activity remaining
718670
6 - 7
-
relative enzyme activity is retained at pH 6.0 and 7.0 after incubating for 12 h at 4°C
756271
6.5 - 11
purified recombinant enzyme, room temperature, 2 h, 90-100% activity remaining
718670
6.5 - 8.7
recombinant enzyme in cell-free extract, room temperature, 2 h, 70% activity remaining at pH 6.5 and pH 8.7, profile overview
718670
8.5
-
24 h, about 30% loss of activity
758526
additional information