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Literature summary for 3.5.5.1 extracted from

  • Bayer, S.; Birkemeyer, C.; Ballschmiter, M.
    A nitrilase from a metagenomic library acts regioselectively on aliphatic dinitriles (2011), Appl. Microbiol. Biotechnol., 89, 91-98.
    View publication on PubMed
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
gene nit1, DNA and amino acid sequence determination and analysis, expression of His-tagged Nit1 in Escherichia coli strain BL21(DE3) uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
47
-
 glutaronitrile pH 7.0, 30°C, recombinant His-tagged Nit1 uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, about, SDS-PAGE uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium E1A0Z9 gene nit1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged Nit1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration uncultured bacterium

Source Tissue

Source Tissue Comment Organism Textmining
additional information no growth on 4-tolunitrile or 4-hydroxybenzonitrile as sole nitrogen sources uncultured bacterium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylglutaronitrile + 2 H2O regioselectivity of Nit1 uncultured bacterium 4-cyanopentanoic acid + NH3
-
 ?
3-cyanopyridine + 2 H2O
-
 uncultured bacterium pyridine 3-carboxylic acid + NH3
-
 ?
3-phenylpropionitrile + H2O
-
 uncultured bacterium 3-phenylpropionic acid + NH3
-
 ?
cinnamonitrile + H2O
-
 uncultured bacterium ?
-
 ?
glutaronitrile + H2O
-
 uncultured bacterium glutaric acid + NH3
-
 ?
additional information Nit1 substrate specificity, overview. Significant activities are observed with the aromatic substrates 3-phenylpropionitrile and cinnamonitrile. To a minor degree, the heterocyclic 3-cyanopyridine is also accepted as substrate. In contrast to aliphatic dinitriles, the aliphatic mononitriles acetonitrile, propionitrile, and butyronitrile are almost not converted by Nit1, no activity on malononitrile uncultured bacterium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, about, SDS-PAGE uncultured bacterium

Synonyms

Synonyms Comment Organism
NIT1
-
 uncultured bacterium
nitrilase
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 recombinant His-tagged Nit1 uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified recombinant His-tagged Nit1, 1 h, stable uncultured bacterium
50
-
 purified recombinant His-tagged Nit1, 30 min, loss of 50% activity uncultured bacterium
60
-
 purified recombinant His-tagged Nit1, 10 in, complete inactivation uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant His-tagged Nit1 uncultured bacterium

pH Range

pH Minimum pH Maximum Comment Organism
5 9 over 50% of maximal activity within this range uncultured bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.48
-
 glutaronitrile pH 7.0, 30°C, recombinant His-tagged Nit1 uncultured bacterium