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Literature summary for 3.5.5.1 extracted from

  • Li, H.; Yang, T.; Gong, J.S.; Xiong, L.; Lu, Z.M.; Li, H.; Shi, J.S.; Xu, Z.H.
    Improving the catalytic potential and substrate tolerance of Gibberella intermedia nitrilase by whole-cell immobilization (2015), Bioprocess Biosyst. Eng., 38, 189-197.
    View publication on PubMed
Search for more literature for 3.5.5.1

General Stability

General Stability Organism
maximal nitrilase activity of alginate-immobilized cells is obtained under conditions of 2% (w/v) alginate, 0.6% (w/v) CaCl2, 0.4 g cell/g alginate, and 1.8 mm bead size Fusarium proliferatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-cyanopyridine + 2 H2O Fusarium proliferatum
-
 nicotinic acid + NH3
-
 ?
3-cyanopyridine + 2 H2O Fusarium proliferatum CA3-1
-
 nicotinic acid + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium proliferatum
-
-
-
Fusarium proliferatum CA3-1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + 2 H2O
-
 Fusarium proliferatum nicotinic acid + NH3
-
 ?
3-cyanopyridine + 2 H2O
-
 Fusarium proliferatum CA3-1 nicotinic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
NIT1
-
 Fusarium proliferatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 free and immobilized enzyme Fusarium proliferatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the half-lives of alginate-immobilized cells at 30, 40 and 50°C are 315, 117.5 and 10.9 h, respectively Fusarium proliferatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 free enzyme Fusarium proliferatum
8
-
 immobilized enzyme Fusarium proliferatum