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3.5.5.1: nitrilase

This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.

Word Map on EC 3.5.5.1

Reaction

A nitrile
+ 2 H2O =
a carboxylate
 +
NH3

Synonyms

3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.1 nitrilase

Specific Activity

Specific Activity on EC 3.5.5.1 - nitrilase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0006
pH 7.4, 70°C, substrate: benzonitrile
0.0037
-
pH 7.0, 90°C, substrate: 2-cyanopyridine
0.075
-
cell extract, pH 9.0, 30°C
0.12
-
benzonitrilase B
0.14
mutant enzyme A198V/I290F/H135Y/Y213H/T350S/Y177C/A285T, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 7.5 and 30°C
0.24
recombinant enzyme expressed without GroEL/ES, pH 8.0, 30°C
0.28
recombinant enzyme expressed without GroEL/ES, pH 8.0, 30°C
0.4
-
enzyme in cells, substrate 2-methyl-2-phenylpropionitrile in Na/K-phosphate buffer, pH 7.0, 30°C
0.52
mutant enzyme A198V/I290F/H135Y/Y213H/T350S/Y177C/A285T, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 4.5 and 30°C
0.6
-
mutant enzyme N164A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
0.62
wild type enzyme, using (R)-2-chloromandelonitrile as substrate, in 50 mM potassium phosphate buffer, at pH 7.5 and 30°C
0.64
recombinant enzyme coexpressed with GroEL/ES, pH 8.0, 30°C
0.7
recombinant enzyme coexpressed with GroEL/ES, pH 8.0, 30°C
1.26
-
pH 7.5
1.31
-
benzonitrilase A
1.9
enzyme in cells, substrate 2-methyl-2-phenylpropionitrile in Na/K-phosphate buffer, pH 7.0, 30°C
10
-
mutant enzyme S188A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
10.4
-
mutant enzyme I104A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
13.5
-
mutant enzyme Y142A, using 2-cyanopyridine as a substrate
14.1
-
enzyme in cells, substrate benzonitrile in Na/K-phosphate buffer, pH 7.0, 30°C
14.5
-
mutant enzyme R130A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
14.6
pH 8.0, 37°C, substrate: benzonitrile
15.3
-
for benzonitrile as substrate, for benzamide as substrate very low, but significant specific acitivity
15.7
-
-
17.7
-
mutant enzyme R130A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
18.2
-
mutant enzyme K9A, using m-tolunitrile as a substrate
19
substrate mandelonitrile, purified recombinant enzyme, pH 8.0, 40°C
19.8
-
mutant enzyme L79A, using benzonitrile as a substrate
25
-
mutant enzyme L79A, using m-tolunitrile as a substrate
25.6
purified enzyme, using mandelonitrile as substrate, at 30°C and pH 8.0
29
-
mutant enzyme L79A, using 2-cyanopyridine as a substrate
3.6
-
mutant enzyme Y175A, using benzonitrile as a substrate
3286.7
-
subatrate: benzonitrile, pH 8.0, 80°C
4.9
-
crude extract, at pH 8.0 and 45°C
44
enzyme activity in immobilized cells, pH and temperature not specified in the publication
5
-
mutant enzyme Y175A, using m-tolunitrile as a substrate
5.1
-
mutant enzyme V49A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
5.2
-
mutant enzyme M114A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
7.4
-
mutant enzyme K9A, using benzonitrile as a substrate
7.5
-
mutant enzyme T115A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
8
-
mutant enzyme S188A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
8.2
-
mutant enzyme T115A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
9
-
mutant enzyme Q116A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
9.2
-
wild type enzyme, using 2-cyanopyridine as a substrate
9.6
-
mutant enzyme G103A, using 3-methylbenzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
9.7
-
mutant enzyme N164A, using benzonitrile as substrate, in 50 mM potassium phosphate (pH 7.5), at 25°C
91.6
-
after 18.7fold purification, at pH 8.0 and 45°C
additional information
-
after 20.3fold purification, the specific activity is 144 units/mg, while the crude enzyme exhibits a specific activity of 7.1 units/mg at pH 8.0 and 45°C