3.5.5.1: nitrilase

This is an abbreviated version!
For detailed information about nitrilase, go to the full flat file.

Reaction

Synonyms

3-cyanopyridinase, acetonitrilase, Arylacetonitrilase, auxin-producing nitrilase, benzonitrilase, benzonitrilase A, benzonitrilase B, bll6402, BrNIT-T, CrNIT1, CrNIT2, cyc705, CynD, humanNIT1, More, nirilase II, NIT, NIT-T2, Nit06, NIT1, Nit1 nitrilase, Nit102, NIT2, NIT3, NIT4A/B1, NIT4A/B2, NitA, NitAk1, nitmc-fb, NitraS-ATII, nitrilase, nitrilase 1, nitrilase AtNIT1, nitrilase bll6402, nitrilase I, nitrile hydratase/amidase, Nlase, PaCNit, SsAH, SSO2122

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.5 In nitriles

                3.5.5.1 nitrilase

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Results	in table
22	Activating Compound
841	AA Sequence
74	Application
7	CAS Registry Number
54	Cloned(Commentary)
1	Crystallization (Commentary)
23	Disease/ Diagnostics
10	Expression
77	General Information
12	General Stability
241	Inhibitors
31	kcat/KM [mM/s]
3	Ki Value [mM]
148	KM Value [mM]
16	Localization
21	Metals/Ions
104	Molecular Weight [Da]
85	Natural Substrates/ Products (Substrates)
49	Organic Solvent Stability
572	Organism
10	Pathway
24	PDB ID
83	pH Optimum
28	pH Range
20	pH Stability
3	pI Value
5	Posttranslational Modification
147	Protein Variants
60	Purification (Commentary)
7	Reaction
5	Reaction Type
336	Reference
2	Renatured (Commentary)
44	Source Tissue
73	Specific Activity [micromol/min/mg]
3	Storage Stability
1017	Substrates and Products (Substrate)
108	Subunits
79	Synonyms
1	Systematic Name
73	Temperature Optimum [°C]
20	Temperature Range [°C]
59	Temperature Stability [°C]
66	Turnover Number [1/s]

Activating Compound

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Activating Compound on EC 3.5.5.1 - nitrilase

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ACTIVATING COMPOUND

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

2-Cyanopyridine

Fusarium solani

-

nitrilase inducer

713440

acetone

Pannonibacter carbonis

-

5% (v/v), 1.2fold activation

756271

Benzonitrile

Rhodococcus sp.

-

-

712260

dithiothreitol

Pannonibacter carbonis

-

1 mM, 1.2fold activation

756271

DTT

Pyrococcus sp. MC-FB

-

1 mM

756683

EDTA

5 entries

glycerin

Pannonibacter carbonis

-

1% (v/v), 1.3fold activation

756271

hydroxylamine

Rhodococcus rhodochrous

-

20% activation at 1 mM

209755

indole-3-acetonitrile

Zea mays

-

induces protein expression

688087

L-cysteine

2 entries

L-cystine

Aeribacillus pallidus

-

16% activation at 1 mM

209746

L-glutathione

Pannonibacter carbonis

-

1 mM, 1.3fold activation

756271

NaHS

Aeribacillus pallidus

-

19% activation at 1 mM

209746

SDS

Pannonibacter carbonis

-

1% (v/v), 1.4fold activation

756271

Span-80

Pannonibacter carbonis

-

1% (v/v), 1.2fold activation

756271

-

Urea

Aspergillus niger

-

19% increase of activity at 1% (w/v)

684594

additional information

Zea mays

-

a 2fold induction by light is observed

688087

-

EDTA

Acidovorax facilis

Q1W2L4

activates wild-type nitrilase and mutant enzyme F168V/T201N/S192F/M191T/F192S

756946

EDTA

Alcaligenes sp.

C5IIS9

slightly activating at 1 mM, not at 5 mM

719102

EDTA

Fusarium proliferatum

A0A0E3D8K7

1 mM, 1.3fold stimulation

756840

EDTA

Fusarium proliferatum

-

114% activity at 1 mM

735075

EDTA

Pannonibacter carbonis

-

1 mM, 1.35fold activation

756271

L-cysteine

Pannonibacter carbonis

-

1 mM, 1.3fold activation

756271

L-cysteine

Pyrococcus sp. MC-FB

-

1 mM

756683