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Literature summary for 3.5.5.1 extracted from

  • Cai, W.; Su, E.; Zhu, S.; Ren, Y.; Wei, D.
    Characterization of a nitrilase from Arthrobacter aurescens CYC705 for synthesis of iminodiacetic acid (2014), J. Gen. Appl. Microbiol., 60, 207-214.
    View publication on PubMed
Search for more literature for 3.5.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paenarthrobacter aurescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
43.17
-
 iminodiacetonitrile at pH 7.2 and 30°C Paenarthrobacter aurescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36700
-
 14 * 36700, MALDI TOF mass spectrometry Paenarthrobacter aurescens
37000
-
 14 * 37000, SDS-PAGE Paenarthrobacter aurescens
520000
-
 gel filtration Paenarthrobacter aurescens

Organism

Organism UniProt Comment Textmining
Paenarthrobacter aurescens S5M6X9
-
-
Paenarthrobacter aurescens CYC705 S5M6X9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Paenarthrobacter aurescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-phenylenediacetonitrile + H2O 0.063% activity compared to iminodiacetonitrile Paenarthrobacter aurescens ? + NH3
-
 ?
1,4-dicyanobutane + H2O 27.1% activity compared to iminodiacetonitrile Paenarthrobacter aurescens ? + NH3
-
 ?
2-cyanopyridine + 2 H2O 3.27% activity compared to iminodiacetonitrile Paenarthrobacter aurescens pyridine 2-carboxylic acid + NH3
-
 ?
3-hydroxyglutaronitrile + H2O 1.15% activity compared to iminodiacetonitrile Paenarthrobacter aurescens ? + NH3
-
 ?
3-hydroxypropionitrile + H2O 2.57% activity compared to iminodiacetonitrile Paenarthrobacter aurescens 3-hydroxypropionic acid + NH3
-
 ?
3-phenylpropionitrile + H2O 7.24% activity compared to iminodiacetonitrile Paenarthrobacter aurescens 3-phenylpropionic acid + NH3
-
 ?
3-phenylpropionitrile + H2O 7.24% activity compared to iminodiacetonitrile Paenarthrobacter aurescens CYC705 3-phenylpropionic acid + NH3
-
 ?
4-chlorobutyronitrile + H2O 11.1% activity compared to iminodiacetonitrile Paenarthrobacter aurescens 4-chlorobutyric acid + NH3
-
 ?
4-cyanopyridine + 2 H2O 2.48% activity compared to iminodiacetonitrile Paenarthrobacter aurescens pyridine 4-carboxylic acid + NH3
-
 ?
butanedinitrile + H2O 104.8% activity compared to iminodiacetonitrile Paenarthrobacter aurescens ? + NH3
-
 ?
cinnamonitrile + H2O 7.87% activity compared to iminodiacetonitrile Paenarthrobacter aurescens cinnamic acid + NH3
-
 ?
iminodiacetonitrile + 2 H2O 100% activity Paenarthrobacter aurescens iminodiacetic acid + 2 NH3
-
 ?
iminodiacetonitrile + 2 H2O 100% activity Paenarthrobacter aurescens CYC705 iminodiacetic acid + 2 NH3
-
 ?
malononitrile + H2O 78.9% activity compared to iminodiacetonitrile Paenarthrobacter aurescens malonic acid + NH3
-
 ?
malononitrile + H2O 78.9% activity compared to iminodiacetonitrile Paenarthrobacter aurescens CYC705 malonic acid + NH3
-
 ?
mandelonitrile + 2 H2O 2.38% activity compared to iminodiacetonitrile Paenarthrobacter aurescens mandelic acid + NH3
-
 ?
mandelonitrile + 2 H2O 2.38% activity compared to iminodiacetonitrile Paenarthrobacter aurescens CYC705 mandelic acid + NH3
-
 ?
additional information no activity with benzonitrile, phenylacetonitrile, 3-cyanopyridine, glycolonitrile, and dodecanenitrile Paenarthrobacter aurescens ?
-
 ?
additional information no activity with benzonitrile, phenylacetonitrile, 3-cyanopyridine, glycolonitrile, and dodecanenitrile Paenarthrobacter aurescens CYC705 ?
-
 ?
valeronitrile + H2O 4.9% activity compared to iminodiacetonitrile Paenarthrobacter aurescens valeric acid + NH3
-
 ?

Subunits

Subunits Comment Organism
homotetradecamer 14 * 36700, MALDI TOF mass spectrometry Paenarthrobacter aurescens
homotetradecamer 14 * 37000, SDS-PAGE Paenarthrobacter aurescens

Synonyms

Synonyms Comment Organism
cyc705
-
 Paenarthrobacter aurescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Paenarthrobacter aurescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 the yield of product decreases sharply after the enzyme is heat-treated at 40°C for 30 min and 1 h. The enzyme completely loses its activity after heat-treatment at 60°C for 30 min. However, this enzyme is relatively stable from 20 to 30°C Paenarthrobacter aurescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.57
-
 iminodiacetonitrile at pH 7.2 and 30°C Paenarthrobacter aurescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Paenarthrobacter aurescens

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.8 highly active between pH 5.5 and 7.8 Paenarthrobacter aurescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0363
-
 iminodiacetonitrile at pH 7.2 and 30°C Paenarthrobacter aurescens