Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 114% activity at 1 mM | Fusarium proliferatum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta-gami (DE3) cells | Fusarium proliferatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Fusarium proliferatum | |
Ba2+ | about 76% residual activity at 1 mM | Fusarium proliferatum | |
Ca2+ | about 77% residual activity at 1 mM | Fusarium proliferatum | |
Co2+ | 1 mM decreases the enzyme activity to about 90% | Fusarium proliferatum | |
Cu2+ | about 75% residual activity at 1 mM | Fusarium proliferatum | |
ethanol | a relative activity of 49% is detected in 5% (v/v) of ethanol | Fusarium proliferatum | |
Fe2+ | about 79% residual activity at 1 mM | Fusarium proliferatum | |
Li+ | about 70% residual activity at 1 mM | Fusarium proliferatum | |
methanol | the enzyme is relatively active in 5% (v/v) methanol with 60% of the enzyme activity remaining | Fusarium proliferatum | |
Ni2+ | 1 mM decreases the enzyme activity to about 90% | Fusarium proliferatum | |
Pb2+ | about 78% residual activity at 1 mM | Fusarium proliferatum | |
Propanol | a relative activity of 6% is detected in 5% (v/v) of propanol | Fusarium proliferatum | |
Zn2+ | 1 mM decreases the enzyme activity to about 90% | Fusarium proliferatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.11 | - |
3-Cyanopyridine | at pH 7.2 and 30°C | Fusarium proliferatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 110% activity at 1 mM | Fusarium proliferatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35940 | - |
x * 35940, calculated from amino acid sequence | Fusarium proliferatum |
37000 | - |
x * 37000, SDS-PAGE | Fusarium proliferatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + 2 H2O | Fusarium proliferatum | 100% activity, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine | nicotinic acid + NH3 | - |
? | |
4-cyanopyridine + 2 H2O | Fusarium proliferatum | 69.25% activity compared to 3-cyanopyridine, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine | pyridine 4-carboxylic acid + NH3 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | when 20% (v/v) of organic solvent is used, the enzyme activity sharply decreases and only can be detected with very low activity except that in methanol and 2-chloromethane (40% and 10% of activity retaining, respectively) | Fusarium proliferatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium proliferatum | - |
- |
- |
Fusarium proliferatum CA3-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Fusarium proliferatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloro-3-cyanopyridine + H2O | 0.21% activity compared to 3-cyanopyridine | Fusarium proliferatum | ? | - |
? | |
2-chloro-4-cyanopyridine + H2O | 7.48% activity compared to 3-cyanopyridine | Fusarium proliferatum | ? | - |
? | |
3-cyanopyridine + 2 H2O | 100% activity, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine | Fusarium proliferatum | nicotinic acid + NH3 | - |
? | |
4-cyanopyridine + 2 H2O | 69.25% activity compared to 3-cyanopyridine, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine | Fusarium proliferatum | pyridine 4-carboxylic acid + NH3 | - |
? | |
4-cyanopyridine + 2 H2O | 0.07% activity compared to 3-cyanopyridine | Fusarium proliferatum | pyridine 4-carboxylic acid + NH3 | - |
? | |
acetonitrile + H2O | 2.31% activity compared to 3-cyanopyridine | Fusarium proliferatum | acetate + NH3 | - |
? | |
acetonitrile + H2O | 2.31% activity compared to 3-cyanopyridine | Fusarium proliferatum CA3-1 | acetate + NH3 | - |
? | |
acrylonitrile + H2O | 43.96% activity compared to 3-cyanopyridine | Fusarium proliferatum | acrylic acid + NH3 | - |
? | |
acrylonitrile + H2O | 43.96% activity compared to 3-cyanopyridine | Fusarium proliferatum CA3-1 | acrylic acid + NH3 | - |
? | |
adiponitrile + H2O | 106.29% activity compared to 3-cyanopyridine | Fusarium proliferatum | adipic acid + NH3 | - |
? | |
adiponitrile + H2O | 106.29% activity compared to 3-cyanopyridine | Fusarium proliferatum CA3-1 | adipic acid + NH3 | - |
? | |
benzonitrile + 2 H2O | 42.14% activity compared to 3-cyanopyridine | Fusarium proliferatum | benzoic acid + NH3 | - |
? | |
glycinonitrile + H2O | 23.27% activity compared to 3-cyanopyridine | Fusarium proliferatum | ? | - |
? | |
iminodiacetonitrile + 2 H2O | 58.07% activity compared to 3-cyanopyridine | Fusarium proliferatum | iminodiacetic acid + 2 NH3 | - |
? | |
iminodiacetonitrile + 2 H2O | 58.07% activity compared to 3-cyanopyridine | Fusarium proliferatum CA3-1 | iminodiacetic acid + 2 NH3 | - |
? | |
isovaleronitrile + H2O | 47.17% activity compared to 3-cyanopyridine | Fusarium proliferatum | isovaleric acid + NH3 | - |
? | |
additional information | no activity with mandelonitrile and geranyl nitrile | Fusarium proliferatum | ? | - |
? | |
additional information | no activity with mandelonitrile and geranyl nitrile | Fusarium proliferatum CA3-1 | ? | - |
? | |
phenylacetonitrile + 2 H2O | 21.59% activity compared to 3-cyanopyridine | Fusarium proliferatum | phenylacetate + NH3 | - |
? | |
succinonitrile + H2O | 64.22% activity compared to 3-cyanopyridine | Fusarium proliferatum | succinic acid + NH3 | - |
? | |
valeronitrile + H2O | 45.07% activity compared to 3-cyanopyridine | Fusarium proliferatum | valeric acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Fusarium proliferatum |
? | x * 35940, calculated from amino acid sequence | Fusarium proliferatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Fusarium proliferatum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | the nitrilase activity gradually increases from 20°C to 45°C and is lost rapidly above 50°C | Fusarium proliferatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Fusarium proliferatum |