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Literature summary for 3.5.5.1 extracted from
- Characterization of a nitrilase and a nitrile hydratase from Pseudomonas sp. UW4 that converts indole-3-acetonitrile to produce indole-3-acetic acid (2014), Appl. Environ. Microbiol., 80, 4640-4649.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Pseudomonas sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32848 | - |
x * 32848, calculated from amino acid sequence | Pseudomonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | K9NKH3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni Sepharose 6 column chromatography | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| indole-3-acetonitrile + H2O | - |
Pseudomonas sp. | indole-3-acetic acid + NH3 | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32848, calculated from amino acid sequence | Pseudomonas sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NIT | - |
Pseudomonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudomonas sp. |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas sp. | calculated from amino acid sequence | - |
5.7 |
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