3.5.4.12: dCMP deaminase
This is an abbreviated version!
For detailed information about dCMP deaminase, go to the full flat file.
Word Map on EC 3.5.4.12
Reaction
Synonyms
2-deoxycytidylate deaminase, 5-mdCMP deaminase, 5-methyldeoxycytidine monophosphate deaminase, DCD, DCD1, dCDA, dCMP deaminase, dCMP-aminohydrolase, dCMP-dCTP deaminase, dCMPase, dCMPD, deaminase, deoxycytidylate, deoxy-CMP-deaminase, deoxycytidine deaminase, deoxycytidine monophosphate deaminase, deoxycytidine nucleotide deaminase, deoxycytidine-5'-monophosphate aminohydrolase, deoxycytidine-5'-monophosphate deaminase, deoxycytidine-5'-phosphate deaminase, deoxycytidylate aminohydrolase, deoxycytidylate deaminase, ST2 protein, STRIPE2, T2-dCMP deaminase, T2-deoxycytidylate deaminase, T2-phage deoxycytidylate deaminase, T4-dCMP deaminase, T4-deaminase, T4-phage deoxycytidylate deaminase
ECTree
3.5.4.12 dCMP deaminaseAdvanced search results
results (
results (Inhibitors
Inhibitors on EC 3.5.4.12 - dCMP deaminase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
(4Z,7Z,10Z,13Z,16Z,19Z)-henicosa-4,7,10,13,16,19-hexaenoic acid
-
decreases the enzyme activity in vivo in transformed NM-16 fibroblasts, but increases it in normal Rat-2 fibroblasts
2',2'-difluorodeoxycytidine
-
high cellular nucleotide levels: inhibition: Concentration-dependent inhibition; inhibition by nucleotides of 2',2'-difluorodeoxycytidine, dual mechanism of inhibition
2'-beta-D-deoxyribose-pyrimidin-2-one 5'-phosphate
-
competitive inhibition, Ki: 0.000012 mM
D-beta-2'-fluoro-5-methyl-arabinofuranosyluracil monophosphate
-
no inhibition by L-beta-2'-fluoro-5-methyl-arabinofuranosyluracil monophosphate
deoxythymidine 5'-triphosphate
Tequatrovirus T4
-
allosteric feedback regulation of the enzyme by products of the metabolic pathway, allosteric regulation involved residues 112 and 115
glycerol
-
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition, removed by dTTP
H4dUMP
inhibits both dCTP and dCMP deaminase activities
Hg(CH3COO)2
-
Hg(acetate)2; mercuroacetate, potent inhibitor, 0.0002 mM: 26% inhibition, 0.001 mM: 40% inhibition
PDRP
inhibits both dCTP and dCMP deaminase activities
3,4,5,6-tetrahydro-2'-dUMP
-
high-affinity inhibitor, rapid, reversible inhibition, kinetics of inhibition. At low substrate or competitor concentrations: activation. Tetrahydro-dUMP activates enzyme at low substrate concentrations
5-Hg-dCMP
-
irreversible competitive inhibitor, in the absence of mercaptoethanol
5-Hg-dCMP
-
potent inhibitor, in the absence of 2-mercaptoethanol, 0.00024 mM: 37% inhibition, 0.0024 mM: 70% inhibition
dAMP
-
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
dAMP
-
at low substrate, dCMP, concentration: activation at higher concentration of either: competitive inhibition; competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
dAMP
-
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
dCTP
-
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
dCTP
-
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
dTTP
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells
dTTP
-
allosteric inhibitor; maximum inhibition at 0.015 mM, inhibition requires presence of Mg2+ or Mn2+
dTTP
-
0.01 mM: 100% inhibition. In the presence of glutaraldehyde enzyme is less sensitive to inhibitory effect of dTTP; allosteric inhibitor; inhibited form: dTTP-enzyme; kinetics of inhibition
dTTP
-
allosteric inhibitor; allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
dTTP
-
allosteric inhibitor; cooperative inhibition, reversed by dCTP, mechanism of inhibition
dTTP
-
allosteric inhibitor; inhibition by dTTP increases markedly as the pH is raised, pH-dependent inhibition
dTTP
-
allosteric inhibitor; dTTP favores disaggregated or inhibited state
dTTP
Herpes simplex virus
-
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to inhibition by dTTP than enzyme from non-infected cells
dTTP
-
0.0015 mM: 50% inhibition. dTTP inhibition reversed by dCTP; allosteric inhibitor
dTTP
-
allosteric inhibitor; specifically markedly inhibited, Ki: 0.0017 mM
dTTP
inhibits dCMP deamination, feedback inhibition
dTTP
Tequatrovirus T4
-
allosteric inhibitor; wild-type, 0.1 mM dTTP: 20% inhibition, mutants R115E and R115Q: 0.1 mM dTTP: no inhibition. 0.3 mM dTTP: wild-type 90% inhibition, mutants R115E and R115Q: 30% inhibition
dTTP
Tequatrovirus T4
-
allosteric inhibitor; mutant F112A, no or very slight inhibition, high concentration, 0.24 mM: slight inhibition
Glutaraldehyde
-
25% glutaraldehyde: rapid inhibition; dCTP protects enzyme to a certain extent. Glutaraldehyde fixes dCMPase in the activated conformation induced by dCTP
Glutaraldehyde
-
25% glutaraldehyde: rapid inhibition; substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
TTP
-
activating enzyme up to 1.7fold at concentration of 0.00025 mM, but inhibiting at higher concentration, 0.015 mM: 85% inhibition; inhibition by TTP has absolute requirement for the presence of a divalent cation, Mn2+ and Ca2+ are more effective than Mg2+ in the inhibition of enzyme activity by TTP; TTP inhibition partially reversed by dCTP
additional information
-
little or no inhibition by 3,4,5,6-tetrahydrouridine; mechanism of inhibition
-
additional information
-
inhibitory potency of pyrimidine phosphate analogues, overview
-
additional information
-
monophosphates of deoxycytidine analogs nucleosides in the L-conformation (e.g. apricitabine, lamivudine, and emtricitabine) and dideoxycytidine do not act as inhibitors of dCMPD
-
additional information
-
no inhibition by dATP, dGTP, 5-bromo-UTP
-
