3.5.4.12: dCMP deaminase

This is an abbreviated version!
For detailed information about dCMP deaminase, go to the full flat file.

Reaction

Synonyms

2-deoxycytidylate deaminase, 5-mdCMP deaminase, 5-methyldeoxycytidine monophosphate deaminase, DCD, DCD1, dCDA, dCMP deaminase, dCMP-aminohydrolase, dCMP-dCTP deaminase, dCMPase, dCMPD, deaminase, deoxycytidylate, deoxy-CMP-deaminase, deoxycytidine deaminase, deoxycytidine monophosphate deaminase, deoxycytidine nucleotide deaminase, deoxycytidine-5'-monophosphate aminohydrolase, deoxycytidine-5'-monophosphate deaminase, deoxycytidine-5'-phosphate deaminase, deoxycytidylate aminohydrolase, deoxycytidylate deaminase, ST2 protein, STRIPE2, T2-dCMP deaminase, T2-deoxycytidylate deaminase, T2-phage deoxycytidylate deaminase, T4-dCMP deaminase, T4-deaminase, T4-phage deoxycytidylate deaminase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.4 In cyclic amidines

                3.5.4.12 dCMP deaminase

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Results	in table
118	Activating Compound
977	AA Sequence
6	Application
1	CAS Registry Number
12	Cloned(Commentary)
1	Cofactor
6	Crystallization (Commentary)
264	Disease/ Diagnostics
3	General Information
44	General Stability
129	Inhibitors
6	Ki Value [mM]
59	KM Value [mM]
1	Localization
63	Metals/Ions
30	Molecular Weight [Da]
87	Natural Substrates/ Products (Substrates)
83	Organism
5	Pathway
12	PDB ID
31	pH Optimum
4	pH Range
20	Protein Variants
33	Purification (Commentary)
90	Reaction
1	Reaction Type
71	Reference
3	Renatured (Commentary)
46	Source Tissue
19	Specific Activity [micromol/min/mg]
16	Storage Stability
238	Substrates and Products (Substrate)
43	Subunits
49	Synonyms
1	Systematic Name
23	Temperature Optimum [°C]
1	Temperature Range [°C]
12	Temperature Stability [°C]
12	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 3.5.4.12 - dCMP deaminase

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

109500

Homo sapiens

-

gel filtration

288999

110000 - 122000

Tequatrovirus T4

-

recombinant wild-type enzyme, gel filtration, dependent on protein concentration, in presence and absence of fdCTP

289015

111900

Tequatrovirus T4

-

F112A, HPLC gel filtration

289015

113100

Equus asinus

-

amino acid analysis

289012

115000

Cricetinae

-

BHK-21/C13 cells, sucrose density gradient centrifugation

289008

117000

Homo sapiens

-

in the presence of dCTP and Mg2+, 4°C, gel filtration

289018

120000

2 entries

124000

Escherichia phage T2

-

PAGE and sedimentation equilibrium

289004, 289009, 289010

124500

Tequatrovirus T4

-

R115Q, HPLC gel filtration

289015

127000

Tequatrovirus T4

-

wild-type, HPLC gel filtration

289015

130000

Cricetinae

-

BHK-21/C13 cells, gel filtration

289008

17000

Paramecium bursaria Chlorella virus 1

O41078

6 * 17000, SDS-PAGE

688732

170000

Bacillus subtilis

-

gel filtration and sucrose density gradient centrifugation

289005

18842

Equus asinus

-

6 * 18842, amino acid analysis

289012

20000

3 entries

20200

Escherichia phage T2

-

6 * 20200, sedimentation in 6 M guanidine hydrochloride containing 0.1 M 2-mercaptoethanol and SDS-PAGE

289010

21200

2 entries

38200 - 113000

Tequatrovirus T4

-

recombinant mutant F112A, gel filtration, dependent on protein concentration, in presence and absence of fdCTP

289015

39800 - 118000

Tequatrovirus T4

-

recombinant mutant R115Q, gel filtration, dependent on protein concentration, in presence and absence of fdCTP

289015

41600 - 45400

Tequatrovirus T4

-

recombinant mutant R115E, gel filtration, dependent on protein concentration, in presence and absence of fdCTP

289015

44300

Tequatrovirus T4

-

R115E, HPLC gel filtration

289015

53000

Homo sapiens

-

2 * 53000, SDS-PAGE

288999

120000

Equus asinus

-

-

289002, 289003

120000

Equus asinus

-

PAGE

288998

20000

Homo sapiens

-

6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence

289018, 289020

20000

Homo sapiens

-

6 * 20000, SDS-PAGE

289018

20000

Equus asinus

-

6 * 20000, SDS-PAGE

288998

21200

Tequatrovirus T4

-

6 * 21200, wild-type, particularly in the presence of dCTP: hexamer, mutants R115Q and F112A varies from hexamers to dimers or something in between depending on protein concentration, HPLC gel filtration in the presence of SDS

289015

21200

Tequatrovirus T4

-

2 * 21200, mutant R115E: dimer, mutants R115Q and F112A varies from hexamers to dimers or something in between depending on protein concentration, HPLC gel filtration in the presence of SDS

289015