Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5-hydroxymethyl-dCTP | natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP | Tequatrovirus T4 | |
dCTP | positive allosteric effector | Homo sapiens | |
dCTP | positive allosteric effector | Tequatrovirus T4 | |
dCTP | wild-type, dCTP required for activity, no activity in absence of dCTP and Mg2+ | Tequatrovirus T4 | |
dCTP | mutant F112A, dCTP not required | Tequatrovirus T4 |
Cloned (Comment) | Organism |
---|---|
cloned and amplified in a high-expression system, expression in Escherichia coli strain BL21-DE3/pLysS | Tequatrovirus T4 |
cloned and overexpressed in Escherichia coli strain BL21-DE3/pLysS | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F112A | as active as wild-type even in the absence of dCTP and only weakly inhibited by dTTP, loss of allosteric regulation by dCTP and dTTP, no activation by dCTP, no inhibition by dTTP | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | allosteric inhibitor | Homo sapiens | |
dTTP | allosteric inhibitor; mutant F112A, no or very slight inhibition, high concentration, 0.24 mM: slight inhibition | Tequatrovirus T4 | |
dUMP | - |
Homo sapiens | |
dUMP | - |
Tequatrovirus T4 | |
dUTP | - |
Homo sapiens | |
dUTP | allosteric inhibitor | Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | involved in catalytic mechanism involving His104, and Cys132 and Cys135 | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCMP + H2O | Homo sapiens | - |
dUMP + NH3 | - |
? | |
dCMP + H2O | Tequatrovirus T4 | critical branchpoint in pyrimidine deoxynucleotide metabolism | dUMP + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Tequatrovirus T4 | - |
wild-type and mutant F112A | - |
Tequatrovirus T4 | - |
T4-phage infected Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
cloned wild-type and mutant F112A | Tequatrovirus T4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Homo sapiens | |
dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Tequatrovirus T4 | |
dCMP + H2O = dUMP + NH3 | feedback regulation | Homo sapiens | |
dCMP + H2O = dUMP + NH3 | feedback regulation | Tequatrovirus T4 | |
dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Homo sapiens | |
dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCMP + H2O | - |
Homo sapiens | dUMP + NH3 | - |
? | |
dCMP + H2O | - |
Tequatrovirus T4 | dUMP + NH3 | - |
? | |
dCMP + H2O | critical branchpoint in pyrimidine deoxynucleotide metabolism | Tequatrovirus T4 | dUMP + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 identical subunits | Homo sapiens |
hexamer | 6 identical subunits | Tequatrovirus T4 |
Synonyms | Comment | Organism |
---|---|---|
T4-dCMP deaminase | T4-phage infected | Tequatrovirus T4 |
T4-deaminase | T4-phage infected | Tequatrovirus T4 |
T4-phage deoxycytidylate deaminase | T4-phage infected | Tequatrovirus T4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Tequatrovirus T4 |