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Literature summary for 3.5.4.12 extracted from

  • Moore, J.T.; Ciesla, J.M.; Changchien, L.M.; Maley, G.F.; Maley, F.
    Identification of a site necessary for allosteric regulation in T4-phage deoxycytidylate deaminase (1994), Biochemistry, 33, 2104-2112.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
5-hydroxymethyl-dCTP natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP Tequatrovirus T4
dCTP positive allosteric effector Homo sapiens
dCTP positive allosteric effector Tequatrovirus T4
dCTP wild-type, dCTP required for activity, no activity in absence of dCTP and Mg2+ Tequatrovirus T4
dCTP mutant F112A, dCTP not required Tequatrovirus T4

Cloned(Commentary)

Cloned (Comment) Organism
cloned and amplified in a high-expression system, expression in Escherichia coli strain BL21-DE3/pLysS Tequatrovirus T4
cloned and overexpressed in Escherichia coli strain BL21-DE3/pLysS Homo sapiens

Protein Variants

Protein Variants Comment Organism
F112A as active as wild-type even in the absence of dCTP and only weakly inhibited by dTTP, loss of allosteric regulation by dCTP and dTTP, no activation by dCTP, no inhibition by dTTP Tequatrovirus T4

Inhibitors

Inhibitors Comment Organism Structure
dTTP allosteric inhibitor Homo sapiens
dTTP allosteric inhibitor; mutant F112A, no or very slight inhibition, high concentration, 0.24 mM: slight inhibition Tequatrovirus T4
dUMP
-
Homo sapiens
dUMP
-
Tequatrovirus T4
dUTP
-
Homo sapiens
dUTP allosteric inhibitor Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ involved in catalytic mechanism involving His104, and Cys132 and Cys135 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dCMP + H2O Homo sapiens
-
dUMP + NH3
-
?
dCMP + H2O Tequatrovirus T4 critical branchpoint in pyrimidine deoxynucleotide metabolism dUMP + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
human
-
Tequatrovirus T4
-
wild-type and mutant F112A
-
Tequatrovirus T4
-
T4-phage infected Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
cloned wild-type and mutant F112A Tequatrovirus T4

Reaction

Reaction Comment Organism Reaction ID
dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Homo sapiens
dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Tequatrovirus T4
dCMP + H2O = dUMP + NH3 feedback regulation Homo sapiens
dCMP + H2O = dUMP + NH3 feedback regulation Tequatrovirus T4
dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Homo sapiens
dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCMP + H2O
-
Homo sapiens dUMP + NH3
-
?
dCMP + H2O
-
Tequatrovirus T4 dUMP + NH3
-
?
dCMP + H2O critical branchpoint in pyrimidine deoxynucleotide metabolism Tequatrovirus T4 dUMP + NH3
-
?

Subunits

Subunits Comment Organism
hexamer 6 identical subunits Homo sapiens
hexamer 6 identical subunits Tequatrovirus T4

Synonyms

Synonyms Comment Organism
T4-dCMP deaminase T4-phage infected Tequatrovirus T4
T4-deaminase T4-phage infected Tequatrovirus T4
T4-phage deoxycytidylate deaminase T4-phage infected Tequatrovirus T4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens
30
-
assay at Tequatrovirus T4