Literature summary for 3.5.4.12 extracted from

Almog, R.; Maley, F.; Maley, G.F.; Maccoll, R.; Van Roey, P.
Three-dimensional structure of the R115E mutant of T4-bacteriophage 2-deoxycytidylate deaminase (2004), Biochemistry, 43, 13715-13723.

Activating Compound

Activating Compound	Comment	Organism	Structure
deoxycytidine 5'-triphosphate	allosteric feedback regulation of the enzyme by products of the metabolic pathway, allosteric regulation involved residues 112 and 115	Tequatrovirus T4

Application

Application	Comment	Organism
medicine	the enzyme is a major target for cancer chemotherapy	Tequatrovirus T4

Cloned(Commentary)

Cloned (Comment)	Organism
expression of mutant R115E in Escherichia coli	Tequatrovirus T4

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified mutant R115E free or in complex with active site binding inhibitor pyrimidin-2-one deoxyribotide, the mutant R115E is dimeric in solution, while hexameric in crystals, hanging drop vapour diffusion method, 0.28 mM mutant R115E protein in 20 mM phosphate, pH 7.2, 0.38 mM inhibitor, and 30 mM DTT, is mixed with an equal volume of reservoir solution containing 1.3 M ammonium sulfate, 0.1 M sodium citrate, pH 6.0, and 10 mM DTT, 22°C, 3 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution	Tequatrovirus T4

Crystallization (Comment)

Organism

purified mutant R115E free or in complex with active site binding inhibitor pyrimidin-2-one deoxyribotide, the mutant R115E is dimeric in solution, while hexameric in crystals, hanging drop vapour diffusion method, 0.28 mM mutant R115E protein in 20 mM phosphate, pH 7.2, 0.38 mM inhibitor, and 30 mM DTT, is mixed with an equal volume of reservoir solution containing 1.3 M ammonium sulfate, 0.1 M sodium citrate, pH 6.0, and 10 mM DTT, 22°C, 3 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution

Tequatrovirus T4

Protein Variants

Protein Variants	Comment	Organism
R115E	site-directed mutagenesis, quarternary structure analysis	Tequatrovirus T4

Inhibitors

Inhibitors	Comment	Organism	Structure
deoxythymidine 5'-triphosphate	allosteric feedback regulation of the enzyme by products of the metabolic pathway, allosteric regulation involved residues 112 and 115	Tequatrovirus T4
pyrimidin-2-one deoxyribotide	active site binding structure, overview	Tequatrovirus T4

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the enzyme shows a zinc ion-based reaction mechanism, binding structure and coordinating residues, two zinc ions per subunit, the first is coordinated by His104, Cys132, and Cys135, the second by Cys19, Cys49, His94, and Glu102, overview	Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dCMP + H2O	Tequatrovirus T4	the enzyme is the major supplier of substrate for the thymidylate synthase important in DNA synthesis	dUMP + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant mutant R115E from Escherichia coli	Tequatrovirus T4

Reaction

Reaction	Comment	Organism	Reaction ID
dCMP + H2O = dUMP + NH3	zinc ion-based reaction mechanism, substrate binding structure	Tequatrovirus T4	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dCMP + H2O	-	Tequatrovirus T4	dUMP + NH3	-	?
dCMP + H2O	the enzyme is the major supplier of substrate for the thymidylate synthase important in DNA synthesis	Tequatrovirus T4	dUMP + NH3	-	?

Subunits

Subunits	Comment	Organism
dimer	the mutant R115E is dimeric in solution, while hexameric in crystals, quarternary structure analysis	Tequatrovirus T4

Synonyms

Synonyms	Comment	Organism
2-deoxycytidylate deaminase	-	Tequatrovirus T4
DCD	-	Tequatrovirus T4