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Literature summary for 3.5.4.12 extracted from

  • Sergott, R.C.; Debeer, L.J.; Bessman, M.J.
    On the regulation of a bacterial deoxycytidylate deaminase (1971), J. Biol. Chem., 246, 7755-7758.
    View publication on PubMed
Search for more literature for 3.5.4.12

Activating Compound

Activating Compound Comment Organism Structure
5-hydroxymethyl-dCTP 30% activation Lactobacillus acidophilus
dCTP positive allosteric effector Staphylococcus aureus
dCTP positive allosteric effector Lactobacillus acidophilus
dCTP specifically markedly activated, Km: 0.00017 mM Lactobacillus acidophilus
dGTP very slight activation Lactobacillus acidophilus

General Stability

General Stability Organism
enzyme is unstable Lactobacillus acidophilus

Inhibitors

Inhibitors Comment Organism Structure
2',3'-di-dTTP 7% inhibition Lactobacillus acidophilus
5-bromo-dUTP 92% inhibition Lactobacillus acidophilus
dTTP allosteric inhibitor; specifically markedly inhibited, Ki: 0.0017 mM Lactobacillus acidophilus
dTTP allosteric inhibitor Staphylococcus aureus
dUTP 79% inhibition Lactobacillus acidophilus
additional information no inhibition by dATP, dGTP, 5-bromo-UTP Lactobacillus acidophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dCMP + H2O Staphylococcus aureus
-
 dUMP + NH3
-
 ?
dCMP + H2O Lactobacillus acidophilus
-
 dUMP + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Lactobacillus acidophilus
-
-
-
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Staphylococcus aureus
partial Lactobacillus acidophilus

Reaction

Reaction Comment Organism Reaction ID
dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Staphylococcus aureus
a
dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Lactobacillus acidophilus
a
dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Staphylococcus aureus
a
dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Lactobacillus acidophilus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
-
 Lactobacillus acidophilus

Storage Stability

Storage Stability Organism
on ice, 0.05 M Tris, pH 8.0, 20 mM dithiothreitol, stable for weeks Lactobacillus acidophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCMP + H2O
-
 Staphylococcus aureus dUMP + NH3
-
 ?
dCMP + H2O
-
 Lactobacillus acidophilus dUMP + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Lactobacillus acidophilus