3.5.4.12: dCMP deaminase
This is an abbreviated version!
For detailed information about dCMP deaminase, go to the full flat file.
Word Map on EC 3.5.4.12
Reaction
Synonyms
2-deoxycytidylate deaminase, 5-mdCMP deaminase, 5-methyldeoxycytidine monophosphate deaminase, DCD, DCD1, dCDA, dCMP deaminase, dCMP-aminohydrolase, dCMP-dCTP deaminase, dCMPase, dCMPD, deaminase, deoxycytidylate, deoxy-CMP-deaminase, deoxycytidine deaminase, deoxycytidine monophosphate deaminase, deoxycytidine nucleotide deaminase, deoxycytidine-5'-monophosphate aminohydrolase, deoxycytidine-5'-monophosphate deaminase, deoxycytidine-5'-phosphate deaminase, deoxycytidylate aminohydrolase, deoxycytidylate deaminase, ST2 protein, STRIPE2, T2-dCMP deaminase, T2-deoxycytidylate deaminase, T2-phage deoxycytidylate deaminase, T4-dCMP deaminase, T4-deaminase, T4-phage deoxycytidylate deaminase
ECTree
3.5.4.12 dCMP deaminaseAdvanced search results
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Engineering on EC 3.5.4.12 - dCMP deaminase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
N43G
the mutation results in a dramatic loss of activity with less than 20% activity compared to the wild type
T61A
the mutant shows about 65% activity compared to the wild type enzyme
T61D
the mutant shows about 7% activity compared to the wild type enzyme
T61S
the mutant shows about 145% activity compared to the wild type enzyme
T61V
the mutant shows about 50% activity compared to the wild type enzyme
W42F
the mutant shows about 118% activity compared to the wild type enzyme
Y116F
the mutant shows about 140% activity compared to the wild type enzyme
S178F
-
site-directed mutagenesis, the mutation is responsible for MNNG resistance in the drm1-1 strain,
F112A
R115E
R115Q
additional information
-
construction of a dcd1DELTA enzyme-deficient strain, the deletion strain and the dcd1-S178F mutant strain are crossed with a rad52DELTA mgt1DELTA strain and haploid, double and triple mutant strains are generated, rad52DELTA mgt1DELTA dcd1-S178F and rad52DELTA mgt1DELTA dcd1DELTA strains show an increased level of MNNG resistance, while the only dcd1-deficient strain does not, overview, DCD1 deficiency in yeast results in elevation of dCTP pools and a slight decrease in dTTP pools and reduced the frequency of spontaneous and MNNGinduced G/C to A/T mutations, phenotypes, overview
F112A
Tequatrovirus T4
-
molecular weight analysis using HPLC gel filtration in the presence of SDS and dCTP: wild-type is a hexamer, F112A varies from hexamer to dimer
F112A
Tequatrovirus T4
-
as active as wild-type even in the absence of dCTP and only weakly inhibited by dTTP, loss of allosteric regulation by dCTP and dTTP, no activation by dCTP, no inhibition by dTTP
F112A
Tequatrovirus T4
-
site-directed mutagenesis, the mutant enzyme shows reduced turnover and activity compared to the wild-type enzyme
R115E
Tequatrovirus T4
-
mutant enzyme active in the absence of dCTP and Mg2+, little if any activation by dCTP, possessing turnover number or kcat that is about 15% that of wild-type enzyme, specific activity about 40-50% of wild-type enzyme. Molecular weight analysis using HPLC gel filtration in the presence of SDS and dCTP: wild-type is a hexamer, R115E a dimer
R115E
Tequatrovirus T4
-
site-directed mutagenesis, the mutant enzyme shows reduced turnover and activity compared to the wild-type enzyme, but no longer requires deoxycytidine 5-triphosphate for activation in contrast to the wild-type enzyme
R115Q
Tequatrovirus T4
-
mutant enzyme active in the absence of dCTP and Mg2+, possessing turnover number or kcat that is about 15% that of wild-type enzyme, specific activity about 40-50% of wild-type enzyme. Molecular weight analysis using HPLC size gel filtration in the presence of SDS and dCTP: wild-type is a hexamer, R115Q varies from hexamer to dimer
R115Q
Tequatrovirus T4
-
site-directed mutagenesis, the mutant enzyme shows reduced turnover and activity compared to the wild-type enzyme, but no longer requires deoxycytidine 5-triphosphate for activation in contrast to the wild-type enzyme
