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3.5.4.12: dCMP deaminase

This is an abbreviated version!
For detailed information about dCMP deaminase, go to the full flat file.

Word Map on EC 3.5.4.12

Reaction

dCMP
+
H2O
=
dUMP
 +
NH3

Synonyms

2-deoxycytidylate deaminase, 5-mdCMP deaminase, 5-methyldeoxycytidine monophosphate deaminase, DCD, DCD1, dCDA, dCMP deaminase, dCMP-aminohydrolase, dCMP-dCTP deaminase, dCMPase, dCMPD, deaminase, deoxycytidylate, deoxy-CMP-deaminase, deoxycytidine deaminase, deoxycytidine monophosphate deaminase, deoxycytidine nucleotide deaminase, deoxycytidine-5'-monophosphate aminohydrolase, deoxycytidine-5'-monophosphate deaminase, deoxycytidine-5'-phosphate deaminase, deoxycytidylate aminohydrolase, deoxycytidylate deaminase, ST2 protein, STRIPE2, T2-dCMP deaminase, T2-deoxycytidylate deaminase, T2-phage deoxycytidylate deaminase, T4-dCMP deaminase, T4-deaminase, T4-phage deoxycytidylate deaminase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.12 dCMP deaminase

General Stability

General Stability on EC 3.5.4.12 - dCMP deaminase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes
dCTP and Mg2+ stabilize
-
dCTP and MgCl2 stabilize
-
dCTP or glycerol stabilize
dCTP stabilizes
dCTP stabilizing hexameric state, dTTP destabilizing
dCTP, 0.125 mM, or ethyleneglycol, 20% v/v, stabilize
-
dCTP, or to a lesser extent dTTP, stabilize against heat inactivation, protein denaturants and proteolytic enzymes
-
dithiothreitol, 2 mM, stabilizes
-
EDTA: denaturation, EDTA removes the two resident Zn2+ atoms /subunit
Tequatrovirus T4
-
enzyme extremely labile, stabilized by dCTP /Mg2+
-
enzyme is unstable
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and ethyleneglycol, 20% v/v
-
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilize against heat inactivation
ethyleneglycol prevents protein denaturation during freezing in the presence of 2-mercaptoethanol
-
ethyleneglycol, 10-20% stabilizes
-
ethyleneglycol, 20%, stabilizes against thermal and UV inactivation and denaturation factors with different mechanism of action
-
glycerol stabilizes
glycerol, 15%, stabilizes
-
guanidine hydrochloride, 1 M, complete loss in activity accompanied by disaggregation of enzyme, 6 M: denaturation, complete reactivation with 50 mM 2-mercaptoethanol
-
guanidine-HCl, 6 M, denaturation, complete restoration of activity on removal of denaturant by dilution or dialysis
Tequatrovirus T4
-
highly stable enzyme
-
some free nucleotides, most effective dCTP, stabilize
-
thiols stabilize
thiols, such as 2-mercaptoethanol or dithiothreitol, stabilize, in the absence of thiols the enzyme is highly unstable
-
Zn2+ or dCTP stabilize, protect against inactivation
-