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Literature summary for 3.5.4.12 extracted from
- Deoxycytidylate deaminase from Bacillus subtilis. Purification, characterization, and physiological function (1978), J. Biol. Chem., 253, 3536-3542.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | enzyme requires dCTP, Zn2+, and 2-mercaptoethanol | Bacillus subtilis |  |
| CTP | no activation | Bacillus subtilis | |
| dCDP | 100% activation | Bacillus subtilis | |
| dCTP | dCTP not required and not activared by dCTP | Bacillusphage phiE | |
| dCTP | dCTP not required and not activared by dCTP | bacteriophage SP8 | |
| dCTP | required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol | Bacillus subtilis | |
| dTTP | no activation | Bacillus subtilis | |
| dUTP | 89% activation | Bacillus subtilis | |
| additional information | no activation by ATP, UTP, CTP and dTTP | Bacillus subtilis |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Bacillus subtilis |
| thiols stabilize | Bacillus subtilis |
| thiols, such as 2-mercaptoethanol or dithiothreitol, stabilize, in the absence of thiols the enzyme is highly unstable | Bacillus subtilis |
| Zn2+ or dCTP stabilize, protect against inactivation | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dTTP | no inhibition by dTTP | bacteriophage SP8 | |
| EDTA | completely | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Bacillus subtilis | |
| 0.14 | - |
dCMP | - |
Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no activation | Bacillus subtilis | |
| Co2+ | metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+ | Bacillus subtilis | |
| metal ion | required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+ | Bacillus subtilis | |
| Mg2+ | no activation | Bacillus subtilis | |
| Mg2+ | enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+ | Bacillus subtilis | |
| Mn2+ | metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+ | Bacillus subtilis | |
| additional information | metal ions not required | Bacillusphage phiE | |
| additional information | metal ions not required | bacteriophage SP8 | |
| additional information | metal ion required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+ | Bacillus subtilis | |
| Ni2+ | metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+ | Bacillus subtilis | |
| Zn2+ | required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+ | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 170000 | - |
gel filtration and sucrose density gradient centrifugation | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCMP + H2O | Bacillus subtilis | importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides | dUMP + NH3 | - |
? | |
| dCMP + H2O | Bacillus subtilis ED40 | importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides | dUMP + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis ED40 | - |
- |
- |
| Bacillusphage phiE | - |
host Bacillus subtilis | - |
| bacteriophage SP8 | - |
host Bacillus subtilis | - |
| no activity in Escherichia coli | - |
wild type Escherichia coli contains no dCMP deaminase | - |
| no activity in Salmonella typhimurium | - |
do not contain dCMP deaminase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bacillus subtilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dCMP + H2O = dUMP + NH3 | regulation, allosteric end-product regulation | Bacillus subtilis | |
| dCMP + H2O = dUMP + NH3 | highly regulated allosteric enzyme | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.36 | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, pH 7.8, 2 mM 2-mercaptoethanol | Bacillus subtilis |
| 0°C, 2-mercaptoethanol, stable for more than 6 h | Bacillus subtilis |
| 0°C, half-life: 30 min | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP + H2O | slight activity, about 5% compared with dCMP | Bacillus subtilis | UMP + NH3 | - |
? | |
| CMP + H2O | slight activity, about 5% compared with dCMP | Bacillus subtilis ED40 | UMP + NH3 | - |
? | |
| dCMP + H2O | - |
Bacillus subtilis | dUMP + NH3 | - |
? | |
| dCMP + H2O | - |
Bacillusphage phiE | dUMP + NH3 | - |
? | |
| dCMP + H2O | - |
bacteriophage SP8 | dUMP + NH3 | - |
? | |
| dCMP + H2O | importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides | Bacillus subtilis | dUMP + NH3 | - |
? | |
| dCMP + H2O | - |
Bacillus subtilis ED40 | dUMP + NH3 | - |
? | |
| dCMP + H2O | importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides | Bacillus subtilis ED40 | dUMP + NH3 | - |
? | |
| additional information | substrate specificity | Bacillus subtilis | ? | - |
? | |
| additional information | no activity with dCDP, dCTP, CDP, CTP | Bacillus subtilis | ? | - |
? | |
| additional information | substrate specificity | Bacillus subtilis ED40 | ? | - |
? | |
| additional information | no activity with dCDP, dCTP, CDP, CTP | Bacillus subtilis ED40 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
half-life: 30 min, Zn2+, thiols, 2-mercaptoethanol, and dCTP protect against inactivation | Bacillus subtilis |
| 50 | - |
thiol, 2-mercaptoethanol, dCTP and Zn2+ protect the enzyme against heat inactivation at 50°C | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
pH optimum around | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | 50% maximal activity at pH 7.0 and 8.5 | Bacillus subtilis |
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