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Literature summary for 3.5.4.12 extracted from

  • Raia, C.A.; Nucci, R.; Vaccaro, C.; Sepe, S.; Rella, R.
    Reversal of the effect of the allosteric ligands of dCMP-aminohydrolase and stabilization of the enzyme in the T form (1982), J. Mol. Biol., 157, 557-570.
    View publication on PubMed
Search for more literature for 3.5.4.12

Activating Compound

Activating Compound Comment Organism Structure
ammonium sulfate substrate dCMP-Hg-S-CH2-CH2-OH, 0.2 M ammonium sulfate: activation, reversed by cCTP Equus asinus
dCTP allosteric activator Equus asinus
dCTP allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH Equus asinus
dTTP allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration Equus asinus
Glutaraldehyde substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation Equus asinus
glycerol substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition Equus asinus

General Stability

General Stability Organism
dCTP or glycerol stabilize Equus asinus

Inhibitors

Inhibitors Comment Organism Structure
5-Hg-dCMP irreversible competitive inhibitor, in the absence of mercaptoethanol Equus asinus
dAMP competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate Equus asinus
dCTP allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH Equus asinus
dGMP competitive inhibitor Equus asinus
dTTP allosteric inhibitor; allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH Equus asinus
dUMP competitive inhibitor Equus asinus
Glutaraldehyde 25% glutaraldehyde: rapid inhibition; substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation Equus asinus
glycerol substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition, removed by dTTP Equus asinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic properties and parameters Equus asinus
additional information
-
 additional information kinetic experiments Equus asinus
0.26
-
 dCMP-Hg-S-CH2-CH2-OH
-
 Equus asinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
-
 Equus asinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dCMP + H2O Equus asinus catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis dUMP + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Equus asinus
-
 donkey
-
Equus asinus
-
 three conformational isomers of dCMPase, the conformation of the enzyme with no ligands, the activated form, R form: dCTP-Mg complex and the inhibited form, T form: dTTP-Mg complex
-

Purification (Commentary)

Purification (Comment) Organism
-
 Equus asinus

Reaction

Reaction Comment Organism Reaction ID
dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Equus asinus
a

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Equus asinus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Equus asinus
23
-
 substrate: dCMP-Hg-S-CH2-CH2-OH Equus asinus
820
-
 substrate: dCMP Equus asinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromo-dCMP + H2O
-
 Equus asinus 5-bromo-dUMP + NH3
-
 ?
5-fluoro-dCMP + H2O
-
 Equus asinus 5-fluoro-dUMP + NH3
-
 ?
5-Hg-dCMP + H2O deamination activated by dTTP and inhibited by dCTP Equus asinus 5-Hg-dUMP + NH3
-
 ?
5-Hg-dCMP + H2O 5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol Equus asinus 5-Hg-dUMP + NH3
-
 ?
5-hydroxymethyl-dCMP + H2O
-
 Equus asinus 5-hydroxymethyl-dUMP + NH3
-
 ?
5-iodo-dCMP + H2O
-
 Equus asinus 5-iodo-dUMP + NH3
-
 ?
5-methyl-dCMP + H2O
-
 Equus asinus 5-methyl-dUMP + NH3
-
 ?
ara-CMP + H2O arabinosyl-CMP Equus asinus ara-UMP + NH3
-
 ?
ara-CMP + H2O at a very low rate Equus asinus ara-UMP + NH3
-
 ?
ara-CMP + H2O effective substrate in presence of dCTP Equus asinus ara-UMP + NH3
-
 ?
CMP + H2O poor substrate Equus asinus UMP + NH3
-
 ?
CMP + H2O at a low rare, strongly activated by dCTP Equus asinus UMP + NH3
-
 ?
dCMP + H2O not a substrate of the T form, dTTP-induced form of the enzyme Equus asinus dUMP + NH3
-
 ?
dCMP + H2O catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis Equus asinus dUMP + NH3
-
 ?
dCMP-Hg-S-CH2-CH2-OH + H2O better substrate of T form: in the presence of dTTP Equus asinus dUMP-Hg-S-CH2-CH2-OH + NH3
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 identical subunits Equus asinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 assay at Equus asinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at, substrate: dCMP-Hg-S-CH2-CH2-OH Equus asinus