3.5.4.12: dCMP deaminase
This is an abbreviated version!
For detailed information about dCMP deaminase, go to the full flat file.
Word Map on EC 3.5.4.12
Reaction
Synonyms
2-deoxycytidylate deaminase, 5-mdCMP deaminase, 5-methyldeoxycytidine monophosphate deaminase, DCD, DCD1, dCDA, dCMP deaminase, dCMP-aminohydrolase, dCMP-dCTP deaminase, dCMPase, dCMPD, deaminase, deoxycytidylate, deoxy-CMP-deaminase, deoxycytidine deaminase, deoxycytidine monophosphate deaminase, deoxycytidine nucleotide deaminase, deoxycytidine-5'-monophosphate aminohydrolase, deoxycytidine-5'-monophosphate deaminase, deoxycytidine-5'-phosphate deaminase, deoxycytidylate aminohydrolase, deoxycytidylate deaminase, ST2 protein, STRIPE2, T2-dCMP deaminase, T2-deoxycytidylate deaminase, T2-phage deoxycytidylate deaminase, T4-dCMP deaminase, T4-deaminase, T4-phage deoxycytidylate deaminase
ECTree
3.5.4.12 dCMP deaminaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.5.4.12 - dCMP deaminase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
beta-D-2',2'-difluorodeoxycytidine + H2O
beta-D-2',2'-difluorodeoxyuridine + NH3
-
-
-
-
?
dCMP-Hg-S-CH2-CH2-OH + H2O
dUMP-Hg-S-CH2-CH2-OH + NH3
-
better substrate of T form: in the presence of dTTP
-
-
?
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
-
deamination activated by dTTP and inhibited by dCTP
-
-
?
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
-
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
-
-
?
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
-
very poor substrate in the absence of mercaptoethanol
-
-
?
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
-
deamination activated by dTTP and inhibited by dCTP
-
-
?
5-Hg-dCMP + H2O
5-Hg-dUMP + NH3
-
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
-
-
?
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
-
very poor substrate
-
-
?
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
-
HeLa cells, good substrate
-
-
?
5-hydroxymethyl-dCMP + H2O
5-hydroxymethyl-dUMP + NH3
Tequatrovirus T4
-
very poor substrate
-
-
?
5-iodo-dCMP + H2O
5-iodo-dUMP + NH3
-
very poor substrate
-
-
?
5-methyl-dCMP + H2O
5-methyl-dUMP + NH3
-
5-methyl-dCMP: very poor substrate
-
-
?
ara-CMP + H2O
ara-UMP + NH3
-
deaminated only in the presence of dCTP, strongly activated by dCTP
-
-
?
CMP + H2O
UMP + NH3
-
slight activity, about 5% compared with dCMP
-
-
?
CMP + H2O
UMP + NH3
-
deaminated only in the presence of dCTP, strongly activated by dCTP
-
-
?
CMP + H2O
UMP + NH3
-
HeLa cells, at 1 mM, in presence and absence of dCTP and Mg2+: not a substrate
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
?
dCMP + H2O
dUMP + NH3
Betapolyomavirus macacae
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
Betapolyomavirus macacae
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells
-
?
dCMP + H2O
dUMP + NH3
-
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
not a substrate of the T form, dTTP-induced form of the enzyme
-
?
dCMP + H2O
dUMP + NH3
-
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
true substrate probably dCMP-Mg complex
-
?
dCMP + H2O
dUMP + NH3
-
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme within pyrimidine deoxynucleotide pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme plays an important if not rate controlling step in cell division
-
-
?
dCMP + H2O
dUMP + NH3
-
pathway for synthesis of dUMP, nucleotide required for synthesis of dTMP
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme plays an important if not rate controlling step in cell division
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
the dCMP concentration at half-maximum velocity is lower by enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster than by enzyme from non-infected cells
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
enzyme involved in main pathway leading to synthesis of dTTP, role in provision of precursors for DNA synthesis
-
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
Herpes simplex virus
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
most dramatic allosteric response at substrate levels 0.1 mM dCMP or less
-
?
dCMP + H2O
dUMP + NH3
-
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
key enzyme in pyrimidine deoxyribonucleotide metabolism, catalyzing the rate-limiting step, plays an important role in providing a balanced supply of dCTP and dTTP for DNA synthesis
-
?
dCMP + H2O
dUMP + NH3
-
important role in regulation of intracellular TTP levels
-
-
ir
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
enzyme is major source of mammalian cell dUMP pool and regulates the synthesis of TMP
-
-
ir
dCMP + H2O
dUMP + NH3
-
the enzyme is important in the de novo synthesis of thymidine nucleotides, and may also be involved in the metabolism of anticancer agents
-
-
?
dCMP + H2O
dUMP + NH3
-
the enzyme plays a key role in the thymidylate synthase, pathway overview
-
-
?
dCMP + H2O
dUMP + NH3
-
one of the regulatory enzymes of DNA biosynthesis
-
?
dCMP + H2O
dUMP + NH3
-
enzyme of pyrimidine deoxyribonucleotide metabolism, important for the synthesis of thymidine nucleotides from thymine. Enzyme could provide the major means of dUMP formation
-
?
dCMP + H2O
dUMP + NH3
the enzyme is crucial to the production of dTTP needed for DNA replication and damage repair. It mediates dNTP pool balance required for normal chloroplast development and vegetative growth in plants
-
-
?
dCMP + H2O
dUMP + NH3
-
dUMP is a key intermediate in the synthesis of dTTP and subsequently of DNA synthesis
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
-
deoxycytidine nucleotides are shunted into dTMP biosynthetic pathway via reaction catalyzed by dCMP deaminase
-
?
dCMP + H2O
dUMP + NH3
-
the regulation signal is transmitted by Arg4 from the allosteric site to the active site via modifications in the interactions at the interface where the substrate binding pocket is involved and the relocations of Arg26, His65, Tyr120, and Arg121 to envelope the active site in order to stabilize substrate binding in the complex, allosteric mechanism for enzyme regulation, regulator binding structure, overview
-
-
?
dCMP + H2O
dUMP + NH3
-
a conserved motif, G43YNG46, is involved in the binding of dCTP, N-terminal Arg4, a key residue located between two monomers, binds strongly to the gamma phosphate group of dCTP, active site structure and substrate binding, overview
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
major function to provide supplementary route for dTMP synthesis, located at a branch point in pyrimidine metabolic pathway
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
critical branchpoint in pyrimidine deoxynucleotide metabolism
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
-
-
?
dCMP + H2O
dUMP + NH3
Tequatrovirus T4
-
the enzyme is the major supplier of substrate for the thymidylate synthase important in DNA synthesis
-
-
?
deoxycytidine + H2O
deoxyuridine + NH3
-
the enzyme is a key enzyme in the inactivation of deoxycytidine and several chemotherapeutically important nucleoside analogues
-
-
?
additional information
?
-
-
no activity with dCDP, dCTP, CDP, CTP
-
-
?
additional information
?
-
no deamination activity with dCTP or CMP
-
-
?
additional information
?
-
-
catalyzes deamination of halogenated derivatives of dCMP, but appears to be restricted in its ability to deaminate substrates with a large group in the 5-position
-
-
?
additional information
?
-
-
HeLa cells, no activity with CDP at 1 mM, in presence and absence of dCTP and Mg2+
-
-
?
additional information
?
-
-
enzyme is involved in elimination of some dCyd analogues, such as 2',2'-difluorodeoxycytidine, fluoro-dCyd, and ara-C, from tissues or cultured cells
-
-
?
additional information
?
-
-
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, deamination pathway, overview
-
-
?
additional information
?
-
-
the human cytomegalovirus requires the cellular host dCMP deaminase for replication in quiescent cells, overview
-
-
?
additional information
?
-
-
substrate specificity with deoxycytidine analogue monophosphates, overview
-
-
?
additional information
?
-
-
the enzyme also performs 5-methylcytidyl-DNA deaminase activity but to a lesser extent, substrate specificity, overview
-
-
?
additional information
?
-
-
cytidine monophosphate is not a good substrate for dCMPD. Monophosphates of deoxycytidine analogs nucleosides in the L-conformation (e.g. apricitabine, lamivudine, and emtricitabine) and dideoxycytidine do not act as substrates of dCMPD
-
-
?
additional information
?
-
the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations
-
-
?
additional information
?
-
-
the bifunctional dCMP-dCTP deaminase shows dCMP deaminase and dCTP deaminase activities, dCTP serves as a positive heterotropic effector for the dCMP deaminase activity and a positive homotropic effector for the dCTP deaminase activity, and the enzymatic efficiency of the dCMP deaminase activity is about four times higher than that of the dCTP deaminase activity, the same active site is involved in both dCMP and dCTP deaminations
-
-
?
additional information
?
-
-
halogenated deoxycytidylates: very poor substrates
-
-
?
additional information
?
-
Tequatrovirus T4
-
function of enzyme is that of enhancing stability of T4-phage multienzyme complex involved in dNTP synthesis, the complex is less stable in cd mutants
-
-
?
