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3.5.1.88: peptide deformylase

This is an abbreviated version!
For detailed information about peptide deformylase, go to the full flat file.

Word Map on EC 3.5.1.88

Reaction

formyl-L-methionyl peptide
+
H2O
=
formate
 +
methionyl peptide

Synonyms

AtDEF1.1, AtDEF1.2, AtDEF2, AtPDF1A, AtPDF1B, AtPDF1Bt, AtPDF2, BbPDF, BcPDF, BcPDF2, DEF, Def1, DEF2, deformylase, peptide N-formylmethionine, EC 3.5.1.27, EcPDF, ECPDF1B, EfPDF, HpPDF, HsPDF, hydrolase, aminoacyl-transfer ribonucleate, LiPDF, mPDF, Ni-peptide deformylase, PDF, PDF-1, PDF-2, PDF1A, PDF1B, PDF2, PdfA, PdfB, PdfC, peptide deformylase, peptide deformylase 1, peptide deformylase 1A, peptide deformylase 1B, peptide deformylase 2, Pf PDF, PfPDF, Polypeptide deformylase, SaPDF, sPDF, TbPDF1, TbPDF2, type I PDF, type II PDF, type II peptide deformylase, Vp 16 PDF1B, Vp16 PDF, Vp16T, XOO1075, XoPDF

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.88 peptide deformylase

Cloned

Cloned on EC 3.5.1.88 - peptide deformylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a truncated HsPDF lacking the first 63 amino acids, which correspond to the mitochondrial targeting sequence, is cloned into the vector pET-15b for expression in Escherichia coli BL21DE3 pLys cells
AtDEF1.1 is expressed in Nicotiana tabacum. Overexpression of AtDEF1.1 does not result in resistance to actinonin
-
AtDEF1.2 is expressed in Nicotiana tabacum. Overexpression of AtDEF1.2 results in resistance to actinonin
-
AtDEF2 is expressed in Nicotiana tabacum. Overexpression of AtDEF1.2 results in resistance to actinonin
-
cloning of the nuclear encoded gene of the apicoplast-localized PfPDF, functional recombinant expression in Escherichia coli
Def and YkrB, expression in Escherichia coli
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, recombinant expression
-
enzyme from Escherichia coli strain NCIM 2931
Escherichia coli chromosomal def gene is cloned into the pLysS expression plasmid for co-transformation with pET-based expression vectors
-
expressed as His-tag fusion protein in Escherichia coli
expressed in an Escherichia coli deficiency strain
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli strain ArcticExpress
-
expressed in HEK-293 cells
-
expressed in Nicotiana tabacum chloroplasts
-
expression in Escherichia coli
expression in Escherichia coli with a C-terminal six-histidine tag (BbPDF1-HT)
-
for expession in Escherichia coli C41(DE3) cells with a C-terminal eight-residue, LEHHHHHH, tag
-
for over-expression in Escherichia coli
-
for over-expression in Escherichia coli BL21(DE3) cells
gene def or gbs1883, recombinant expression of enzyme mutant S2A in Escherichia coli strain Rosetta 2(DE3)pLysS
gene def, recombinant enzyme expression
gene def, recombinant expression of the enzyme fused to an octaglutamate tag using expression vector pBAD/Myc-His-DEST in Escherichia coli TOP10 cells and strain JM109
gene def, recombinant expression of the enzyme, containing an N-terminal His6-tag followed by a Tobacco etch virus protease cleavage site and three alanine residues before the NdeI site to facilitate purification of the expressed protein, in Escherichia coli strain BL21(DE3)
-
gene def, sequence comparisons, recombinant expression
gene def, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene DEF2, recombinant expression of isozyme 1B
GST-tagged original PDF protein and thioredoxin-tagged original PDF are expressed in both Escherichia coli BL21 (DE3) and Rosetta (DE3) strains, respectively
-
high-level expression in Escherichia coli
into a pET-vector for expression in Escherichia coli Bl21DE3 cells
-
into pET-vector for expression in Escherichia coli strain BL21(DE3)
-
into pET28a vector for overexpression of the recombinant protein in Escherichia BL21(DE3) cells
-
into pET28c vector for over-expression in Escherichia coli strain BL21(DE3)
-
into pET29b and pET22b vector for expression in Escherichia coli BL21(DE3) cells
-
into the pGEM vector, a GFP cassette is added in frame
into the vector pET28a for expression in Escherichia coli BL21DE3 cells
mutant lacking the first 63 amino acids is expressed in Escherichia coli
-
orf60T, phylogenetic analysis
overexpressed in Escherichia coli BL21(DE3) cells
-
overexpression in Escherichia coli
PdfA is cloned and overepressed in Escherichia coli
-
PdfB is cloned and overepressed in Escherichia coli
-
PdfC is cloned and overepressed in Escherichia coli
-
peptide deformylase 1 and 2, expression in Escherichia coli
-
recombinant expression in Escherichia coli strains PAL421Tr (PDF knockout) and Rosetta2(DE3)pLysS
recombinant overexpression of C-terminally His6-tagged enzyme in Escherichia coli strain C41 (DE3)
-
sequence comparison, recombinant expression of C-terminally His6-tagged human-like PDF protein, composed of 197 residues
the coding region of amino-acid residues 43-212 is cloned into the vector pET11a for expression in Escherichia coli BL21DE3 cells
-
truncated form that lacks the N-terminal 58-amino-acid targeting sequence is overexpressed in Escherichia coli as a Co2+-substituted protein, is catalytically active in deformylating N-formylated peptides. Expression of HsPDF fused to the enhanced green fluorescence protein in human embryonic kidney cells
-