Information on EC 3.5.1.88 - peptide deformylase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.5.1.88
-
RECOMMENDED NAME
GeneOntology No.
peptide deformylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
formyl-L-methionyl peptide + H2O = formate + methionyl peptide
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
deformylation
-
-
deformylation
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
deformylation
-
-
deformylation
-
-
deformylation
B6RGY0, -, Q5VNN5
;
formylation
-
-
hydrolysis of amide bond
-
-
-
-
hydrolysis of amide bond
-
-
hydrolysis of amide bond
-
-
hydrolysis of amide bond
-
-
hydrolysis of amide bond
-
-
hydrolysis of amide bond
-
-
hydrolysis of amide bond
-
-
hydrolysis of amide bond
-
-
hydrolysis of amide bond
-
-
hydrolysis of amide bond
-, Q672W7
-
hydrolysis of amide bond
-
-
hydrolysis of amide bond
-
-
SYSTEMATIC NAME
IUBMB Comments
formyl-L-methionyl peptide amidohydrolase
Requires Fe(II). Also requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions. Differs in substrate specifity from EC 3.5.1.27 (N-formylmethionylaminoacyl-tRNA deformylase) and EC 3.5.1.31 (formylmethionine deformylase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AtDEF1.1
-
-
AtDEF1.2
-
-
AtDEF2
-
-
AtPDF1A
Q9FV53
-
AtPDF1B
Q9FUZ2
-
BcPDF2
-
-
deformylase, peptide N-formylmethionine
-
-
-
-
hydrolase, aminoacyl-transfer ribonucleate
-
-
-
-
Ni-peptide deformylase
P68826
-
PDF
-
-
-
-
PDF
-
-
PDF
Chlamydia trachomatis GR10
-
-
-
PDF
Staphylococcus aureus ATCC25923, Staphylococcus aureus ATCC29213, Staphylococcus aureus ATCC43300
-
-
-
PDF
Staphylococcus epidermidis ATCC12228
-
-
-
PDF-1
Q5HQ78
isoform
PDF-2
Q5HPX6
isoform without peptide deformylase activity
PDF1A
-
-
PDF1B
Q9FUZ2
-
PdfA
Legionella pneumophila Philadelphia 1
-
-
-
PdfB
Legionella pneumophila Philadelphia 1
-
-
-
PdfC
Legionella pneumophila Philadelphia 1
-
-
-
peptide deformylase
-
-
peptide deformylase
-
-
peptide deformylase
-
-
peptide deformylase
-
-
peptide deformylase
-
type I and type II
peptide deformylase
-
-
peptide deformylase
-
-
peptide deformylase
-
-
peptide deformylase
-
-
peptide deformylase
-
-
peptide deformylase
B6RGY0, Q5VNN5
-
peptide deformylase
-
-
peptide deformylase
Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
peptide deformylase
-
-
peptide deformylase
-
-
peptide deformylase 1
-
-
peptide deformylase 1A
B6RGY0
-
peptide deformylase 1B
Q5VNN5
-
peptide deformylase 2
-
-
Polypeptide deformylase
-
-
-
-
type II peptide deformylase
-
-
CAS REGISTRY NUMBER
COMMENTARY
369636-51-1
-
37289-08-0
-
9032-86-4
-
9054-98-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
peptide deformylase 1 and 2
-
-
Manually annotated by BRENDA team
two classes of PDF: PDF1A and PDF1B
-
-
Manually annotated by BRENDA team
harbours two functional peptide deformylase: Def and YkrB. YkrB probably is the predominant deformylase
-
-
Manually annotated by BRENDA team
Chlamydia trachomatis GR10
-
-
-
Manually annotated by BRENDA team
C-terminally truncated recombinant enzyme
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
3D homology model of the matured soybean PDF2 based on the X-ray structure of the homologous enzyme from Plasmodium falciparum
-
-
Manually annotated by BRENDA team
strain SS1
Uniprot
Manually annotated by BRENDA team
strain Philadelphia 1
-
-
Manually annotated by BRENDA team
Legionella pneumophila Philadelphia 1
strain Philadelphia 1
-
-
Manually annotated by BRENDA team
no activity in Caenorhabditis elegans
-
-
-
Manually annotated by BRENDA team
no activity in Saccharomyces cerevisiae
-
-
-
Manually annotated by BRENDA team
; enzyme form 1 and 2
-
-
Manually annotated by BRENDA team
-
Q9I7A8
Uniprot
Manually annotated by BRENDA team
strain ATCC27853
Q9I7A8
Uniprot
Manually annotated by BRENDA team
two functional deformylases
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa ATCC27853
strain ATCC27853
Q9I7A8
Uniprot
Manually annotated by BRENDA team
two classes of PDF: PDF1A and PDF1B
-
-
Manually annotated by BRENDA team
strain ATCC2913
Uniprot
Manually annotated by BRENDA team
strain RN4220
Uniprot
Manually annotated by BRENDA team
two functional deformylases
-
-
Manually annotated by BRENDA team
Staphylococcus aureus ATCC25923
-
-
-
Manually annotated by BRENDA team
Staphylococcus aureus ATCC2913
strain ATCC2913
Uniprot
Manually annotated by BRENDA team
Staphylococcus aureus ATCC29213
-
-
-
Manually annotated by BRENDA team
Staphylococcus aureus ATCC43300
-
-
-
Manually annotated by BRENDA team
Staphylococcus aureus RN4220
strain RN4220
Uniprot
Manually annotated by BRENDA team
Staphylococcus enteridis
-
-
-
Manually annotated by BRENDA team
isoform PDF-1; strain ATCC35984/RP62A
UniProt
Manually annotated by BRENDA team
isoform PDF-2; strain ATCC35984/RP62A
Q5HPX6
UniProt
Manually annotated by BRENDA team
Staphylococcus epidermidis ATCC12228
-
-
-
Manually annotated by BRENDA team
Staphylococcus non-hemolyticus
-
-
-
Manually annotated by BRENDA team
strain ATCC6305
Uniprot
Manually annotated by BRENDA team
two functional deformylases
-
-
Manually annotated by BRENDA team
Streptococcus pneumoniae ATCC6305
strain ATCC6305
Uniprot
Manually annotated by BRENDA team
two functional deformylases
-
-
Manually annotated by BRENDA team
strain MSB8
Uniprot
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
peptide deformylase (PDF) is a protein of the N-terminal methionine excision pathway that removes formylmethionine from mitochondrial-encoded proteins. PDF is crucial in maintaining mitochondrial function
physiological function
-
PDF is an essential and highly conserved enzyme that functions in protein maturation by removing the N-formyl group from the methionine of nascently synthesized polypeptides
physiological function
-
PDF plays a critical role in mediating the maturation process of the nascent polypeptides partly due to the necessity of removing the N-formyl group to render nascent polypeptides available for cleavage of the N-terminal methionine residue by methionine amino peptidase
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(RS)-phenylalanine nitrile + formate
(S)-N-formyl-phenylalanine nitrile
show the reaction diagram
-
-
99% (S)-enantiomer
-
r
2-formyloxycaproylleucyl-p-nitroanilide + H2O
?
show the reaction diagram
-
esterase activity, 10fold lower activity compared to N-formyl-Met-Leu-p-nitroanilide
-
-
?
formyl-L-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
-
-
formyl-L-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
-
?
formyl-L-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + L-methionyl peptide
show the reaction diagram
-, Q672W7
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + L-methionyl peptide
show the reaction diagram
-
peptide deformylase catalyzes the removal of N-terminal formyl groups from nascent ribosome-synthesized polypeptides
-
-
?
formyl-L-methionyl peptide + H2O
formate + L-methionyl peptide
show the reaction diagram
-
theoretical study of the catalytic mechanism and metal-ion dependence of peptide deformylase
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
Q9HBH1
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-MAHA + H2O
formate + MAHA
show the reaction diagram
-
peptide substrate derived from mitochondrial cytochrome c oxidase subunit II
-
-
?
formyl-MEHQ + H2O
formate + MEHQ
show the reaction diagram
-
-
-
-
?
formyl-Met-Ala + H2O
formate + Met-Ala
show the reaction diagram
-
-
-
-
?
formyl-Met-Ala + H2O
formate + Met-Ala
show the reaction diagram
-
substrate activity assay
-
-
?
formyl-Met-Ala + H2O
formate + Met-Ala
show the reaction diagram
Legionella pneumophila Philadelphia 1
-
-
-
-
?
formyl-Met-Ala-His-Ala + H2O
formate + methionyl-Ala-His-Ala
show the reaction diagram
Q9HBH1
fMAHA
-
-
?
formyl-Met-Ala-His-Ala-Ala-Gln + H2O
formate + Met-Ala-His-Ala-Ala-Gln
show the reaction diagram
-
-
-
-
?
formyl-Met-Ala-Lys-Tyr + H2O
formate + Met-Ala-Lys-Tyr
show the reaction diagram
Legionella pneumophila, Legionella pneumophila Philadelphia 1
-
-
-
-
?
formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-, Q672W7
-
-
-
?
formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
Legionella pneumophila Philadelphia 1
-
-
-
-
?
formyl-Met-Ala-Ser + H2O
formate + methionyl-Ala-His-Ala
show the reaction diagram
Q9HBH1
fMAS
-
-
?
formyl-Met-Gly-Gly-CH3 + H2O
formate + Met-Gly-Gly-CH3
show the reaction diagram
-
substrate used in QM-MM model
-
-
?
formyl-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
-
?
formyl-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
-
?
formyl-Met-Thr-Gln-Ser-His + H2O
formate + Met-Thr-Gln-Ser-His
show the reaction diagram
-
-
-
-
?
formyl-Met-Thr-Met-His + H2O
formate + methionyl-Thr-Met-His
show the reaction diagram
Q9HBH1
fMTMH
-
-
?
formyl-Met-Thr-Met-His-Thr-Thr + H2O
formate + Met-Thr-Met-His-Thr-Thr
show the reaction diagram
-
-
-
-
?
formyl-Met-Thr-Pro-Met-Arg-Lys + H2O
?
show the reaction diagram
-
-
-
-
?
formyl-MFAD + H2O
formate + MFAD
show the reaction diagram
-
peptide substrate derived from mitochondrial cytochrome c oxidase subunit I
-
-
?
formyl-MFHQ + H2O
formate + MMFQ
show the reaction diagram
-
-
-
-
?
formyl-MGHQ + H2O
formate + MGHQ
show the reaction diagram
-
-
-
-
?
formyl-MLHQ + H2O
formate + MLHQ
show the reaction diagram
-
-
-
-
?
formyl-MLKL + H2O
formate + MLKL
show the reaction diagram
-
peptide substrate derived from mitochondrial NADH dehydrogenase subunit 4
-
-
?
formyl-MMYA + H2O
formate + MMYA
show the reaction diagram
-
peptide substrate derived from mitochondrial NADH dehydrogenase subunit 6
-
-
?
formyl-MNFA + H2O
formate + MNFA
show the reaction diagram
-
peptide substrate derived from mitochondrial NADH dehydrogenase subunit 3
-
-
?
formyl-MNPL + H2O
formate + MNPL
show the reaction diagram
-
peptide substrate derived from mitochondrial NADH dehydrogenase subunit 2
-
-
?
formyl-MPQL + H2O
formate + MPQL
show the reaction diagram
-
peptide substrate derived from mitochondrial ATP synthase F0 subunit 8
-
-
?
formyl-MRHQ + H2O
formate + MRHQ
show the reaction diagram
-
-
-
-
?
formyl-MTHQ + H2O
formate + MTHQ
show the reaction diagram
-
-, peptide substrate derived from mitochondrial cytochrome c oxidase subunit III
-
-
?
formyl-MTMH + H2O
formate + MTMH
show the reaction diagram
-
peptide substrate derived from mitochondrial NADH dehydrogenase subunit 5
-
-
?
formyl-MTPM + H2O
formate + MTPM
show the reaction diagram
-
peptide substrate derived from mitochondrial cytochrome b
-
-
?
N-(alpha-difluoroacetyl)-Met-Leu-p-nitroanilide + H2O
difluoroacetic acid + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
?
N-(alpha-fluoroacetyl)-Met-Leu-p-nitroanilide + H2O
fluoroacetic acid + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
?
N-formyl-1-(1-naphthyl)ethylamine + H2O
(1R)-1-naphthalen-1-ylethanamine + formate
show the reaction diagram
-
-
90% (R)-enantiomer
-
?
N-formyl-3-amino-3-phenylpropionic acid + H2O
(R)-3-amino-3-phenylpropionic acid + formate
show the reaction diagram
-
-
100% (R)-enantiomer
-
?
N-formyl-Ala-Gly-Ser-Glu + H2O
formate + Ala-Gly-Ser-Glu
show the reaction diagram
-
poor activity, preference of N-terminal methionine
-
?
N-formyl-Ala-Gly-Ser-Glu + H2O
formate + Ala-Gly-Ser-Glu
show the reaction diagram
-
poor activity, preference of N-terminal methionine
-
?
N-formyl-alaninol + H2O
(S)-alaninol + formate
show the reaction diagram
-
-
85.6% (S)-enantiomer
-
?
N-formyl-alpha-methyl-phenylglycine amide + H2O
(S)-alpha-methyl-phenylglycine amide + formate
show the reaction diagram
-
-
100% (S)-enantiomer
-
?
N-formyl-beta-thiaphenyl-Lys-p-nitroanilide + H2O
formate + thiophenol + NH3 + Lys-p-nitroanilide
show the reaction diagram
-
-
-
?
N-formyl-beta-thiaphenyl-Lys-p-nitroanilide + H2O
formate + thiophenol + NH3 + Lys-p-nitroanilide
show the reaction diagram
-
-
-
?
N-formyl-beta-thiaphenylalanyl-peptide + H2O
formate + thiophenol + NH3 + alanyl-peptide
show the reaction diagram
-
-
-
?
N-formyl-beta-thiaphenylalanyl-peptide + H2O
formate + thiophenol + NH3 + alanyl-peptide
show the reaction diagram
-
significantly poorer substrate than N-formyl-thiaphenylalanylleucyl-p-nitroanilide
-
?
N-formyl-His-Ala-Ser-Arg + H2O
formate + His-Ala-Ser-Arg
show the reaction diagram
-
greatly attenuated activity, preference for N-terminal methionine
-
?
N-formyl-L-Met-D-Ala-Ser-Arg + H2O
formate + L-Met-D-Ala-Ser-Arg
show the reaction diagram
-
strong requirement for L-methionine, but D-amino acids tolerated in other positions
-
?
N-formyl-L-Met-L-Ala-L-Ser + H2O
formate + L-Met-L-Ala-L-Ser
show the reaction diagram
Q5HPX6, Q5HQ78, -
-
-
-
?
N-formyl-L-Met-L-Ala-L-Ser + H2O
formate + L-Met-L-Ala-L-Ser
show the reaction diagram
-, Q672W7
activity assay
-
-
?
N-formyl-L-Met-L-Leu-4-nitroanilide + H2O
formate + L-Met-L-Leu-4-nitroanilide
show the reaction diagram
-
-
-
-
?
N-formyl-L-Met-Val + H2O
formate + L-Met-Val
show the reaction diagram
-
-
-
-
?
N-formyl-L-Met-Val + H2O
formate + L-Met-Val
show the reaction diagram
-
-
-
?
N-formyl-L-Met-Val + H2O
formate + L-Met-Val
show the reaction diagram
-
-
-
?
N-formyl-L-Met-Val + H2O
formate + L-Met-Val
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine + H2O
formate + L-methionine
show the reaction diagram
-
worst substrate
-
-
?
N-formyl-L-methionine polypeptide + H2O
formate + methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine polypeptide + H2O
formate + methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine polypeptide + H2O
formate + methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine polypeptide + H2O
formate + methionine-polypeptide
show the reaction diagram
P68826
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
Q9I7A8
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
Q5HPX6, Q5HQ78, -
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
requires at least a dipeptide as substrate, increasing activity with increasing peptide length until it reaches the tetrapeptide
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
deformylation required for methionine aminopeptidase activity in eubacteria, highly specific
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
deformylase is likely to play an essential role in the chloroplast
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
the enzyme is involved in maturation of proteins by cleaving the N-formyl group from N-blocked methionine polypeptides
-
-
-
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
essential role in protein processing in plastids
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
the enzyme is requiredfor the removal of the formyl group at the N-terminus of nascent polypeptide chains
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
posttranslational deformylation of N-formyl-Met-polypeptide
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
P68826
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
O31410
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-, Q9I7A8
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
Q9F2F0
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
P96113
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-Nle-L-Ala-L-Ser + H2O
formate + L-Nle-L-Ala-L-Ser
show the reaction diagram
-
norleucine is the only excepted substitution for methionine
-
?
N-formyl-Leu-Tle-NHCH3 + H2O
(S)-Leu-(S)-Tle-NHCH3
show the reaction diagram
-
Tle i.e. tert-leucine
Tle i.e. tert-leucine, 94% (S,S)-diastereomer
-
?
N-formyl-m-methoxyphenylalanine nitrile + H2O
(S)-m-methoxyphenylalanine nitrile + formate
show the reaction diagram
-
-
99% (S)-enantiomer
-
?
N-formyl-Met + H2O
formate + Met
show the reaction diagram
-
-
-
?
N-formyl-Met + H2O
formate + Met
show the reaction diagram
-
poor activity
-
?
N-formyl-Met + H2O
formate + Met
show the reaction diagram
-
poor activity
-
?
N-formyl-Met + H2O
formate + Met
show the reaction diagram
-
minimal substrate
-
?
N-formyl-Met + H2O
formate + Met
show the reaction diagram
-
minimal substrate
-
?
N-formyl-Met-Ala + H2O
formate + Met-Ala
show the reaction diagram
-
-
-
?
N-formyl-Met-Ala + H2O
formate + Met-Ala
show the reaction diagram
-
-
-
?
N-formyl-Met-Ala + H2O
formate + Met-Ala
show the reaction diagram
-
-
-
?
N-formyl-Met-Ala + H2O
formate + Met-Ala
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala + H2O
formate + Met-Ala
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala + H2O
Met-Ala + formate
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala(OCH3) + H2O
formate + Met-Ala(OCH3)
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala-Phe-Tyr-beta-Ala-Arg + H2O
formate + Met-Ala-Phe-Tyr-beta-Ala-Arg
show the reaction diagram
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-, Q93LE9
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
best substrate
-
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
-
higher activity than compared to di- or tetrapeptides
-
?
N-formyl-Met-Ala-Ser + H2O
formate + Met-Ala-Ser
show the reaction diagram
Chlamydia trachomatis GR10
-
-
-
-
?
N-formyl-Met-Leu-Glu + H2O
formate + Met-Leu-Glu
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Leu-NH2 + H2O
formate + Met-Leu-NH2
show the reaction diagram
-
-
-
?
N-formyl-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
?
N-formyl-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
?
N-formyl-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
?
N-formyl-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
Q9FV53
-
-
-
?
N-formyl-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
best substrate
-
?
N-formyl-Met-Leu-p-nitroanilide + H2O
formate + Met-Leu-p-nitroanilide
show the reaction diagram
-
best substrate
-
?
N-formyl-Met-Leu-Phe + H2O
formate + Met-Leu-Phe
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Leu-Phe + H2O
formate + Met-Leu-Phe
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ser-Asn-Glu + H2O
formate + Met-Ser-Asn-Glu
show the reaction diagram
-
-
-
-
-
N-formyl-Met-Ser-Asn-Glu + H2O
formate + Met-Ser-Asn-Glu
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Ser-Asn-Glu + H2O
formate + Met-Ser-Asn-Glu
show the reaction diagram
-
decreasing activity for tetrapeptides
-
?
N-formyl-Met-Val-Ser + H2O
formate + Met-Val-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Met-Xaa-Xbb-Tyr + H2O
formate + Met-Xaa-Xbb-Tyr
show the reaction diagram
-
consesus sequence for best substrates, Xaa: any amino acid except for Asp and Glu, Xbb: Lys, Arg, Tyr or Phe
-
?
N-formyl-Nle-Ala(NH2) + H2O
formate + Nle-Ala(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Ala(NH2) + H2O
formate + Nle-Ala(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Ala-Ser + H2O
formate + Nle-Ala-Ser
show the reaction diagram
-
-
-
-
-
N-formyl-Nle-Ala-Ser + H2O
formate + Nle-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Arg(NH2) + H2O
formate + Nle-Arg(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Arg(NH2) + H2O
formate + Nle-Arg(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Asn(NH2) + H2O
formate + Nle-Asn(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Asp(NH2) + H2O
formate + Nle-Asp(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Asp(NH2) + H2O
formate + Nle-Asp(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Gln(NH2) + H2O
formate + Nle-Gln(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Glu(NH2) + H2O
formate + Nle-Glu(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Gly(NH2) + H2O
formate + Nle-Gly(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Gly(NH2) + H2O
formate + Nle-Gly(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-His(NH2) + H2O
formate + Nle-His(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Leu(NH2) + H2O
formate + Nle-Leu(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Leu(NH2) + H2O
formate + Nle-Leu(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Lys(NH2) + H2O
formate + Nle-Lys(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Met(NH2) + H2O
formate + Nle-Met(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-NH(CH3) + H2O
formate + Nle-NH(CH3)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-NH2 + H2O
formate + Nle-Nle-NH2
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Nle(NH2) + H2O
formate + Nle-Nle(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Phe(NH2) + H2O
formate + Nle-Phe(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Pro(NH2) + H2O
formate + Nle-Pro(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Ser(NH2) + H2O
formate + Nle-Ser(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Ser(NH2) + H2O
formate + Nle-Ser(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Thr(NH2) + H2O
formate + Nle-Thr(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Trp(NH2) + H2O
formate + Nle-Trp(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Tyr(NH2) + H2O
formate + Nle-Tyr(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nle-Val(NH2) + H2O
formate + Nle-Val(NH2)
show the reaction diagram
-
-
-
-
?
N-formyl-Nva-Ala-Ser + H2O
formate + Nva-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Phe-Ala-Ser + H2O
formate + Phe-Ala-Ser
show the reaction diagram
-
-
-
-
?
N-formyl-Phe-Tyr-Phe + H2O
formate + Phe-Tyr-Phe
show the reaction diagram
-
only N-terminal Phe can replace Met
-
?
N-formyl-Phe-Tyr-Phe-His-beta-Ala-Arg + H2O
formate + Phe-Tyr-Phe-His-beta-Ala-Arg
show the reaction diagram
-
-
-
?
N-formyl-Phe-Tyr-Tyr + H2O
formate + Phe-Tyr-Tyr
show the reaction diagram
-
only N-terminal Phe can replace Met
-
?
N-formyl-phenylalanine nitrile + H2O
(S)-phenylalanine nitrile + formate
show the reaction diagram
-
-
98.8% (S)-enantiomer
-
?
N-formyl-phenylglycine + H2O
(S)-phenylglycine + formate
show the reaction diagram
-
-
99.6% (S)-enantiomer
-
?
N-formyl-phenylglycine amide + H2O
(S)-phenylglycine amide + formate
show the reaction diagram
-
-
99.7% (S)-enantiomer
-
?
N-formyl-phenylglycinol + H2O
(S)-phenylglycinol + formate
show the reaction diagram
-
-
90.5-93.3% (S)-enantiomer, depending on method
-
?
N-formyl-tert-leucine amide + H2O
(S)-tert-leucine amide + formate
show the reaction diagram
-
-
100% (S)-enantiomer
-
?
N-formyl-thiaphenyl-Ala-Leu-p-nitroanilide + H2O
formate + thiophenol + NH3 + Ala-Leu-p-nitroanilide
show the reaction diagram
-
excellent substrate
-
?
N-formyl-valine nitrile + H2O
(S)-valine nitrile + formate
show the reaction diagram
-
-
98.8% (S)-enantiomer
-
?
N-formylmethionine-alanine + H2O
formate + methionyl-alanine
show the reaction diagram
-
-
-
-
?
formyl-MVHQ + H2O
formate + MVHQ
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
overview
-
-
-
additional information
?
-
-
proposed reaction cycle of peptide deformylase
-
-
-
additional information
?
-
-
no activity with formyl-Met-Thr-Pro-Met-Arg-Lys
-
-
-
additional information
?
-
-
no detectable activity with formyl-Met-Leu-Phe and formyl-Met-Leu-Pro
-
-
-
additional information
?
-
-
TbPDF1 is essential for normal growth. TbPDF1 is required for oxidative but not for mitochondrial substrate-level phosphorylation, TbPDF2 does neither affect growth on standard medium nor oxidative phosphorylation. A reduced level of TbPDF2 slows down growth in a medium that selects for highly efficient oxidative phosphorylation
-
-
-
additional information
?
-
-
catalyzes the deformylation of nascent peptides in bacteria
-
-
-
additional information
?
-
-
catalyzes the removal of the N-formyl group from the N-terminus of newly synthesized polypeptides
-
-
-
additional information
?
-
P96275
catalyzes the removal of the N-formyl group from the N-terminus of newly synthesized proteins
-
-
-
additional information
?
-
-
peptide deformylase is involved in the deformylation of the N-formyl group of newly synthesized polypeptides during protein synthesis
-
-
-
additional information
?
-
Staphylococcus aureus, Streptococcus pneumoniae, Staphylococcus epidermidis, Staphylococcus enteridis, Staphylococcus non-hemolyticus
-
peptide deformylase is responsible for the removal of the N-formyl group from the terminal methionine residue in bacterial protein synthesis
-
-
-
additional information
?
-
B6RGY0, -, Q5VNN5
the formyl group is removed from nascent proteins by peptide deformylase
-
-
-
additional information
?
-
-
the formyl moiety of newly synthesized peptides is removed by peptide deformylase
-
-
-
additional information
?
-
-
the removal of the formyl group from formylated methionine residues is facilitated by the action of peptide deformylase
-
-
-
additional information
?
-
-
HsPDF can process, in vitro, N-Met-formylated peptides
-
-
-
additional information
?
-
Q5HPX6, Q5HQ78, -
isoform PDF-2 exhibits no peptide deformylase activity
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
formyl-L-methionyl peptide + H2O
formate + L-methionyl peptide
show the reaction diagram
-, Q672W7
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + L-methionyl peptide
show the reaction diagram
-
peptide deformylase catalyzes the removal of N-terminal formyl groups from nascent ribosome-synthesized polypeptides
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
Q9HBH1
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine polypeptide + H2O
formate + methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine polypeptide + H2O
formate + methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine polypeptide + H2O
formate + methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine polypeptide + H2O
formate + methionine-polypeptide
show the reaction diagram
P68826
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
Q9I7A8
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
Q5HPX6, Q5HQ78, -
-
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
requires at least a dipeptide as substrate, increasing activity with increasing peptide length until it reaches the tetrapeptide
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
deformylation required for methionine aminopeptidase activity in eubacteria, highly specific
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
deformylase is likely to play an essential role in the chloroplast
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
the enzyme is involved in maturation of proteins by cleaving the N-formyl group from N-blocked methionine polypeptides
-
-
-
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
essential role in protein processing in plastids
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
the enzyme is requiredfor the removal of the formyl group at the N-terminus of nascent polypeptide chains
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
posttranslational deformylation of N-formyl-Met-polypeptide
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
P68826
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
O31410
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-, Q9I7A8
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
Q9F2F0
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
-
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
N-formyl-L-methionine-polypeptide + H2O
formate + L-methionine-polypeptide
show the reaction diagram
P96113
involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides
-
-
?
formyl-L-methionyl peptide + H2O
formate + methionyl peptide
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
TbPDF1 is essential for normal growth. TbPDF1 is required for oxidative but not for mitochondrial substrate-level phosphorylation, TbPDF2 does neither affect growth on standard medium nor oxidative phosphorylation. A reduced level of TbPDF2 slows down growth in a medium that selects for highly efficient oxidative phosphorylation
-
-
-
additional information
?
-
-
catalyzes the deformylation of nascent peptides in bacteria
-
-
-
additional information
?
-
-
catalyzes the removal of the N-formyl group from the N-terminus of newly synthesized polypeptides
-
-
-
additional information
?
-
P96275
catalyzes the removal of the N-formyl group from the N-terminus of newly synthesized proteins
-
-
-
additional information
?
-
-
peptide deformylase is involved in the deformylation of the N-formyl group of newly synthesized polypeptides during protein synthesis
-
-
-
additional information
?
-
Staphylococcus aureus, Streptococcus pneumoniae, Staphylococcus epidermidis, Staphylococcus enteridis, Staphylococcus non-hemolyticus
-
peptide deformylase is responsible for the removal of the N-formyl group from the terminal methionine residue in bacterial protein synthesis
-
-
-
additional information
?
-
B6RGY0, -, Q5VNN5
the formyl group is removed from nascent proteins by peptide deformylase
-
-
-
additional information
?
-
-
the formyl moiety of newly synthesized peptides is removed by peptide deformylase
-
-
-
additional information
?
-
-
the removal of the formyl group from formylated methionine residues is facilitated by the action of peptide deformylase
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
Co2+-bound enzyme, highly stable
Co2+
-
cobalt-substituted enzyme
Co2+
-
the Co2+-containing enzyme form is active
Co2+
-, Q672W7
the activity of Co2+-containing enzyme is higher than the activity of Zn2+-containing enzyme
Co2+
-
can replace Fe2+ without loss of activity, enhances stability
Co2+
-
can replace Fe2+ without loss of activity, enhances stability
Co2+
-
can replace Fe2+ without loss of activity, enhances stability
Co2+
-
can replace Fe2+ without loss of activity, enhances stability
Co2+
Q9F2F0
can replace Fe2+ without loss of activity, enhances stability
Co2+
P96113
can replace Fe2+ without loss of activity, enhances stability
Co2+
-, Q672W7
-
Co2+
-
-
Co2+
-
enzymatic activity of Escherichia coli PDF is retained after replacement of the active-site Fe2+ ion with Co2+
Co2+
Q5HPX6, Q5HQ78, -
Co2+-substituted isoform PDF-1 exhibits much higher enzymatic activity than that of Ni2+ and Zn2+ substituted PDF-1
Co2+
-
Co2+ is an optimal metal for increasing the activity of purified thioredoxin-fused PDF
Cu2+
-
the enzyme is strongly activated by Cu2+ with 50% increase of activity at 0.05 mM
Fe
-
PDF1B is an iron enzyme
Fe
-
enzyme form 2 contains up to 1.0 iron atom per polypeptide - 0.77 Fe2+ and 0.2 Fe3+ per polypeptide. The iron is likely coordinated by His132, His136 and Cys90 in the protein
Fe
-
enzyme contains iron
Fe2+
-
sensitive to reactive oxygen
Fe2+
-
natural metal, bound to active site; sensitive to reactive oxygen
Fe2+
-
enzyme contains Fe2+
Fe2+
P68826
required
Fe2+
-
required, irreversible oxidation to Fe3+ results in almost complete loss of activity
Fe2+
P68826
required, irreversible oxidation to Fe3+ results in almost complete loss of activity
Fe2+
Q9F2F0
required, irreversible oxidation to Fe3+ results in almost complete loss of activity
Fe2+
P96113
required, irreversible oxidation to Fe3+ results in almost complete loss of activity
Fe2+
-
Ni2+ or Co2+ leads to the retention of almost full catalytic activity
Fe2+
-
-
Fe2+
-, Q672W7
-
Fe2+
-
theoretically investigation of catalytic mechanism and metal specificity by studying both Fe2+-PDF and Zn2+-PDF. In both forms of PDF, the conserved Glu133 residue is protonated in the reactant complex, and acts as a general acid during the reaction. The initial reaction step is the nucleophilic attack of the metal-bound hydroxide on the carbonyl carbon of the substrate. Calculations indicate that the metal ion in Fe2+-PDF is always pentacoordinated during the reaction process, while that in Zn2+-PDF is only tetrahedrally coordinated and not bound to the substrate in the reactant complex. This difference in their metal coordination is suggested to account for the lower activity of Zn2+-PDF in comparison with Fe2+-PDF
Fe2+
-
pysiological metal ion
Fe2+
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
Fe2+
-
the native metal in Escherichia coli PDF is iron
Fe2+
-
the native enzyme contains iron
Fe2+
-
only Fe2+ and Co2+ ions are capable of supporting the enzyme activity of PDF
Fe2+
-
the imidazole ring of histidine can combine with a metal ion such as iron. The activity of the iron form of the enzyme is higher than the nickel form
Fe3+
-
more stable activity than with Fe2+
Iron
-
iron-containing enzyme
Ni2+
-
450fold increasing activity, stabilizes active state
Ni2+
-
gives insensitivity to oxidation
Ni2+
-
enzyme nearly retains its activity on substitution of the naturally occuring Fe2+ by Ni2+
Ni2+
-
can replace Fe2+ without loss of activity, enhances stability
Ni2+
-
can replace Fe2+ without loss of activity, enhances stability
Ni2+
-
can replace Fe2+ without loss of activity, enhances stability
Ni2+
-
can replace Zn2+
Ni2+
-
can replace Fe2+ without loss of activity, enhances stability
Ni2+
Q9F2F0
can replace Fe2+ without loss of activity, enhances stability
Ni2+
P96113
can replace Fe2+ without loss of activity, enhances stability
Ni2+
-, Q672W7
-
Ni2+
-
PdfA with Zn2+ substituted is much less active than the Ni2+ forms of the enzyme; PdfB with Zn2+ substituted is much less active than the Ni2+ forms of the enzyme; PdfC with Zn2+ substituted is as active as the Ni+ form for the fMA substrate and exhibits substrate specificity different from that of Ni2+ PdfC
Ni2+
-
enzyme resident cofactor
Ni2+
-
enzymatic activity of Escherichia coli PDF is retained after replacement of the active-site Fe2+ ion with Ni2+, the activity of the protein is not affected by Ni2+ concentrations up to 0.1 mM
Ni2+
Q5HPX6, Q5HQ78, -
isoform PDF-1 shows much weaker binding ability towards Ni2+ than towards Co2+ and Zn2+
Ni2+
-
the imidazole ring of histidine can combine with a metal ion such as nickel. The activity of the iron form of the enzyme is higher than the nickel form
Zinc
-
ratio of results of zinc to protein is 1:1
Zn
-
PDF1A is a zinc enzyme, contains 0.8 mol of Zn per mol of enzyme
Zn2+
-
10fold more active than Co2+-prepared enzyme
Zn2+
-
Zn2+-bound enzyme inactive in crystallization analysis
Zn2+
-
cruical for activity
Zn2+
-
Zn2+-bound form not sensitive to oxidation
Zn2+
-
enzyme form 1 contains approximately 1.0 Zn2+ per polypeptide chain, the zinc-containing protein 1 is likely formed during iron depletion caused by overexpression
Zn2+
-
zinc metalloprotease
Zn2+
-
His132 and His136 bind Zn2+ through their Nepsilon1 imidazole nitrogens; location of the active site around the zinc ion; zinc metalloprotease
Zn2+
-, Q672W7
the activity of Co2+-containing enzyme is higher than the activity of Zn2+-containing enzyme
Zn2+
-
required
Zn2+
-, Q672W7
-
Zn2+
-
it contains 1.1 Zn2+ ion/polypeptide
Zn2+
-
PdfA with Zn2+ substituted is much less active than the Ni2+ forms of the enzyme; PdfB with Zn2+ substituted is much less active than the Ni2+ forms of the enzyme; PdfC with Zn2+ substituted is as active as the Ni+ form for the fMA substrate and exhibits substrate specificity different from that of Ni2+ PdfC
Zn2+
-
theoretically investigation of catalytic mechanism and metal specificity by studying both Fe2+-PDF and Zn2+-PDF. In both forms of PDF, the conserved Glu133 residue is protonated in the reactant complex, and acts as a general acid during the reaction. The initial reaction step is the nucleophilic attack of the metal-bound hydroxide on the carbonyl carbon of the substrate. Calculations indicate that the metal ion in Fe2+-PDF is always pentacoordinated during the reaction process, while that in Zn2+-PDF is only tetrahedrally coordinated and not bound to the substrate in the reactant complex. This difference in their metal coordination is suggested to account for the lower activity of Zn2+-PDF in comparison with Fe2+-PDF
Zn2+
-
the Zn2+-PDF complex shows 500fold decreased activity
Zn2+
Q5HPX6, Q5HQ78, -
compared with Co2+-substituted PDF-1, the enzymatic activity of Zn2+-substituted PDF-1 shows a 4fold decrease, PDF-1 binds more Zn2+ (1.36 mol Zn per mol protein) than Co2+ (0.87 mol Co per mol protein), though the activity of Zn-PDF is much lower
Zn2+
P68826
contains zinc in the binding site
Mn2+
-
250fold increase of activity
additional information
-
Ni2+ and Zn2+-containing enzyme forms are inactive
additional information
-
theoretical study of the catalytic mechanism and metal-ion dependence of peptide deformylase
additional information
Q5HPX6, Q5HQ78, -
Co2+-, Zn2+-, or Ni2+-substituted isoform PDF-2 exhibits no enzymatic activity
additional information
-
Zn2+ and Ni2+ are not capable of supporting the enzyme activity of PDF
additional information
-
Co2+, Mg2+, Mn2+ do not significantly affect the enzyme activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(1-hydroxyaminomethyl-2-phenylethyl)carbamic acid tert-butyl ester
Q9FUZ2, Q9FV53
; AtPDF2
(1-hydroxycarbamoylmethyl-2-phenylethyl)carbamic acid tert-butyl ester
Q9FUZ2, Q9FV53
AtPDF2
(2E)-3-(2,5-dihydroxyphenyl)-1-(2-hydroxy-4,6-dimethoxyphenyl)prop-2-en-1-one
P68826
-
-
(2R)-N-[(1S)-1-(dimethylcarbamoyl)-2,2-dimethylpropyl]-2-[[formyl(hydroxy)amino]methyl]hexanamide
-
-
(2R)-N-[(1S)-1-(dimethylcarbamoyl)-2,2-dimethylpropyl]-2-[[formyl(hydroxy)amino]methyl]hexanamide
-
Zn-BbPDF1-HT
(2R)-N-[(1S)-5-amino-1-(hexylcarbamoyl)pentyl]-2-[[formyl(hydroxy)amino]methyl]heptanamide
-
-
(2R)-N-[(3S)-2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-4,4-dimethylpent-1-en-3-yl]-2-[[formyl(hydroxy)amino]methyl]hexanamide
-
-
(2R)-N4-hydroxy-N1-[(2R)-1-[(2R)-2-(hydroxymethyl)pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]-2-pentylbutanediamide
P68826
-
-
(2S)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methoxyphenyl)-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-nitrophenyl)-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(5-methyl-1,3-thiazol-2-yl)-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(pyridin-2-yl)-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-phenyl-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[(1R)-1-phenylethyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[(1S)-1-phenylethyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[5-(trifluoromethyl)-1,3,4-thiadiazol-2-yl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-2-[[(9H-fluoren-9-ylmethoxy)carbonyl]amino]pent-4-ynoic acid
P68826
-
-
(2S)-N-(2-bromophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-N-(2-chlorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-N-(3-bromophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-N-(4-bromophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-N-(4-chlorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-N-(4-fluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-N-(5-fluoro-1-oxidopyridin-2-yl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-N-(5-fluoropyridin-2-yl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S)-N-cyclopropyl-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-2,5-dihydro-1H-pyrrole-2-carboxamide
-
-
(2S,3R,4S,5R,6R)-2-[(5-bromo-1H-indol-3-yl)oxy]-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
P68826
-
-
(2S,3R,4S,5S,6R)-2-[(5-bromo-6-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
P68826
-
-
(3R)-3-[[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]carbonyl]-N-hydroxyheptanamide
P96275
-
(3R)-3-[[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]carbonyl]heptanoic acid
P96275
-
(3R)-3-[[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]carbonyl]-N-hydroxyheptanamide
P96275
-
(3R)-3-[[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]carbonyl]heptanoic acid
P96275
-
(E)-N-phenethyl-3-(3,4-diacetoxyphenyl)acrylamide
-, Q672W7
IC50: 0.00125 mM
(R)-2-[(formyl-hydroxy-amino)-methyl]-hexanoic acid ((S)-5-amino-1-phenylcarbamoyl-pentyl)-amide
-
-
(R)-2-[(formyl-hydroxy-amino)-methyl]-hexanoic acid [(S)-5-amino-1-((R)-2-isopropoxymethyl-pyrrolidine-1-carbonyl)-pentyl]-amide
-
-
(R)-2-[(formyl-hydroxy-amino)-methyl]-hexanoic acid [(S)-5-amino-1-((S)-2-isopropoxymethyl-pyrrolidine-1-carbonyl)-pentyl]-amide
-
-
(RS)- and (SR)-3-[(RS)-benzenesulfinyl]heptanoic acid hydroxyamide
-
mixture of both components, IC50: 0.0001 mM, possible antimicrobial agent
(RS)-3-(phenylsulfonyl)heptanoic acid hydroxyamide
-
IC50: 0.035 micro molar, possible antimicrobial agent
(S)-2-O-(H-phosphonoxy)-L-caproyl-L-Leu-p-nitroanilide
-
similar inhibition of Fe2+- and Co2+-bound enzyme
-
(S)-2-O-(phosphonoxy)-L-caproyl-L-leucyl-p-nitroanilide
-
-
1,10-phenanthroline
-
similar inhibition of Fe2+- and Co2+-bound enzyme
1,10-phenanthroline
-
-
1,10-phenanthroline
-
competitive
1,10-phenanthroline
-
complete inhibition at 5 mM
1,10-phenanthroline
-
competitive with respect to N-formyl-Met-Val; time-dependent inhibition of cobalt-substituted enzyme with loss of the active site metal
1,10-phenanthroline
-
competitive with respect to N-formyl-Met-Val
1,10-phenanthroline
-
-
1,2-Ethanedithiol
-
irreversible
1,3-propanedithiol
-
irreversible
1,5-pentanedithiol
-
-
1-(2-[[formyl(hydroxy)amino]methyl]hexanoyl)-4-methylidene-N,N-dimorpholin-4-yl-L-prolinamide
-
-
-
1-(3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl)-4-methylidene-N,N-dimorpholin-4-yl-L-prolinamide
-
-
-
1-(5-bromo-1H-indol-3-yl)-3-hydroxypropan-2-one
Q9FUZ2, Q9FV53
AtPDF2
1-(5-bromo-2-methyl-1H-indol-3-yl)-N-hydroxymethanamine
-
-
1-(5-bromo-2-methyl-1H-indol-3-yl)-N-hydroxymethanamine
Q819K2
-
1-(5-bromo-2-methyl-1H-indol-3-yl)-N-hydroxymethanamine
-
-
1-(methylamino)-3-[2-(prop-2-en-1-yl)phenoxy]propan-2-ol
P68826
-
-
1-[(2-methoxyphenyl)carbamoyl]prolyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(2-methylphenyl)-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(3-methylphenyl)-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methoxyphenyl)-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methylphenyl)-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-morpholin-4-ylphenyl)-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-piperidin-1-ylphenyl)-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-pyrrolidin-1-ylphenyl)-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(1H-pyrrol-1-yl)phenyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(4-methylpiperazin-1-yl)phenyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(4-oxidomorpholin-4-yl)phenyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(2,2-dimethylpropanoyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(2,2-dimethylpropanoyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(2-methylpropanoyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(2-methylpropanoyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(3-methylbutanoyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(3-methylbutanoyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(cyclopropylcarbonyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(cyclopropylcarbonyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(ethoxycarbonyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(ethylcarbamoyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methoxyacetyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methoxyacetyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methoxycarbonyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methylcarbamoyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(prop-2-en-1-ylcarbamoyl)-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(2-hydroxyethyl)carbamoyl]-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(3-hydroxypropyl)carbamoyl]-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(3-phenylpropyl)carbamoyl]-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(cyclopentyloxy)carbonyl]-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(propan-2-yloxy)carbonyl]-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(tetrahydro-2H-pyran-4-yloxy)carbonyl]-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(1H-imidazol-4-yl)ethyl]carbamoyl]-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyrazin-2-yl)ethyl]carbamoyl]-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-2-yl)ethyl]carbamoyl]-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-3-yl)ethyl]carbamoyl]-L-prolinamide
-
-
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-4-yl)ethyl]carbamoyl]-L-prolinamide
-
-
-
1-[5-bromo-2-methyl-1-(phenylsulfonyl)-1H-indol-3-yl]-N-hydroxymethanamine
-
-
1-[5-bromo-2-methyl-1-(phenylsulfonyl)-1H-indol-3-yl]-N-hydroxymethanamine
Q819K2
-
1-[5-bromo-2-methyl-1-(phenylsulfonyl)-1H-indol-3-yl]-N-hydroxymethanamine
-
-
2',2'-bisepigallocatechin monogallate
-
-
2,3-dimercapto-1-propanesulfonic acid
-
-
2,3-Dimercapto-1-propanol
-
-
2-(1-benzyl-5-chloro-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.002 mM
2-(1-butyl-5-chloro-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.00059 mM
2-(2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.00076 mM
2-(4-benzyl-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)-N-oxoacetamide
P68826
-
-
2-(4-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
; AtPDF2
2-(4-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(4-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
Q819K2
-
2-(4-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(4-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(4-[2-[(2,5-difluorophenyl)amino]-2-oxoethyl]-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)-N-oxoacetamide
P68826
-
-
2-(5-bromo-1-cyclopropyl-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.000069 mM
2-(5-bromo-1H-indol-3-yl)-N-hydroxyacetamide
-
MIC is 0.0031 mg/ml
2-(5-bromo-1H-indol-3-yl)-N-hydroxyacetamide
-
MIC is 0.006 mg/ml
2-(5-bromo-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(5-bromo-1H-indol-3-yl)-N-hydroxyacetamide
Q819K2
-
2-(5-bromo-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(5-bromo-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(5-bromo-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.000098 mM
2-(5-bromo-2-methyl-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(5-bromo-2-methyl-1H-indol-3-yl)-N-hydroxyacetamide
Q819K2
-
2-(5-bromo-2-methyl-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(5-bromo-2-methyl-1H-indolyl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
2-(5-bromo-2-methyl-1H-indolyl)-N-hydroxyacetamide
-
-
2-(5-bromo-2-oxo-1,4-dihydroquinazolin-3(2H)-yl)-N-hydroxyacetamide
-
IC50: 0.000049 mM
2-(5-chloro-1-cyclopropyl-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.00011 mM
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
-
MIC is 0.013 mg/ml
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
-
MIC is 0.025 mg/ml
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
Q819K2
-
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(5-chloro-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
inhibitor shows good selectivity for peptide deformylase over several endoproteases including matrix metalloproteases, however it shows only weak antibacterial activity. IC50: 0.00012 mM
2-(5-chloro-2-oxo-1,4-dihydroquinazolin-3(2H)-yl)-N-hydroxyacetamide
-
inhibitor shows good selectivity for peptide deformylase over several endoproteases including matrix metalloproteases, however it shows only weak antibacterial activity. IC50: 0.00031 mM
2-(5-chloro-2-oxo-1,4-dihydroquinazolin-3(2H)-yl)acetohydrazide
-
IC50: 0.027 mM
2-(5-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
2-(5-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
-
MIC is 0.027 mg/ml
2-(5-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
-
MIC is 0.04 mg/ml
2-(5-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(5-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
Q819K2
-
2-(5-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(5-fluoro-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.00087 mM
2-(5-fluoro-2-oxo-1,4-dihydroquinazolin-3(2H)-yl)-N-hydroxyacetamide
-
IC50: 0.00087 mM
2-(5-methoxy-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
; AtPDF2
2-(6-bromo-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
2-(6-bromo-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(6-bromo-1H-indol-3-yl)-N-hydroxyacetamide
Q819K2
-
2-(6-bromo-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(6-bromo-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2-(biphenyl-4-yl)-N-oxoacetamide
P68826
-
-
2-(indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
; AtPDF2
2-(indol-3-yl)-N-hydroxyacetamide
-
MIC is 0.16 mg/ml
2-(indol-3-yl)-N-hydroxyacetamide
-
MIC is 0.115 mg/ml
2-amino-5-mercapto-1,3,4-thiadiazole
-
slow-binding inhibitor of PDF when dissolved only in dimethylformamide, but not in any other solvent, and aged (via the formation of a disulfide bond) to a dimeric form
-
2-amino-N-[(1S,2S)-1-hydroxy-1-phenylpropan-2-yl]-N-methylacetamide
P68826
-
-
2-butyl-5,7-dimethyl-3-[2''-(1H-tetrazol-5-ylmethyl)-[1,1',4',1'']terphenyl-4-yl]-3H-imidazo[4,5-b]pyridine
-
belongs to a class of angiotensin II receptor antagonists, potent inhibitor, IC50: 0.0076 mM
2-mercaptoethanol
-
30% inhibition at 1 mM
2-oxo-pyrrolidine with chelators
-
-
-
2-thioxo-4-thiazolidinone N-hexanoic acid
-
IC50 against Escherichia coli peptide deformylase: 0.00166 mM
2-thioxo-4-thiazolidinones
-
-
-
2-[(12R,17aS)-1,13-dioxohexadecahydro-1H-pyrrolo[1,2-a][1,4]diazacyclopentadecin-12-yl]-N-hydroxyacetamide
-
-
2-[(3-aminopropanoyl)amino]-3-phenylpropanoic acid
P68826
-
-
2-[(3S,6R)-3-tert-butyl-2,5-dioxo-1,4-diazacyclopentadecan-6-yl]-N-hydroxyacetamide
-
-
2-[2,2-dioxido-5-(trifluoromethyl)-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl]-N-hydroxyacetamide
-
IC50: 0.00013 mM
2-[2-[(2-hydroxyethyl)amino]-1H-benzimidazol-1-yl]-1-phenylethanone
P68826
-
-
2-[4-(3,3-dimethyl-2-oxobutyl)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl]-N-oxoacetamide
P68826
-
-
2-[4-(3-methylbutyl)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl]-N-oxoacetamide
P68826
-
-
2-[4-(cyclobutylmethyl)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl]-N-oxoacetamide
P68826
-
-
2-[5-chloro-2,2-dioxido-1-(3-phenylpropyl)-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl]-N-hydroxyacetamide
-
IC50: 0.00029 mM
3-(3-acetylsulfanyl-2-oxopropyl)indole-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
AtPDF2
3-carboxymethylindol-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
; AtPDF2
3-methoxycatechol
-
-
3-methyl-N-(1,3-thiazol-2-ylcarbamoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
3-methyl-N-(2-methylpropanoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
3-methyl-N-(morpholin-4-ylcarbonyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
3-methyl-N-(phenylcarbamoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
3-methyl-N-(piperidin-1-ylcarbonyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
3-methyl-N-(pyridin-2-ylcarbamoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
3-methyl-N-(pyridin-3-ylcarbamoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
3-methyl-N-(pyridin-4-ylcarbamoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
3-methyl-N-(thiophen-2-ylcarbonyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
3-methyl-N-[methyl(phenyl)carbamoyl]-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
3-[5'-benzyl-2'-(1H-tetrazol-5-yl)-biphenyl-4-yl]-2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine
-
potent inhibitor, IC50: 0.0342 mM, the acidic moiety forms direct ionic interactions with active site metal cation
5'-phosphothiorate modified antisense oligodeoxyribonucleotides
-
-
-
5-(1,1-dimethyl-propyl)-4'-(2-ethyl-5,7-dimethyl-imidazo[4,5-b]pyridin-3-yl)-biphenyl-2-sulfonylcarbamate
-
competitive inhibition, IC50: 0.015 mM, the acidic moiety forms direct ionic interactions with active site metal cation
-
5-bromo-3-hydroxycarbamoylmethylindole-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
AtPDF2
5-bromo-3-hydroxycarbamoylmethylindole-1-carboxylic acid benzyl ester
-
MIC is 0.016 mg/ml
5-bromo-3-hydroxycarbamoylmethylindole-1-carboxylic acid benzyl ester
-
MIC is 0.12 mg/ml
5-chloro-2-propyl-3-[2''-(1H-tetrazol-5-ylmethyl)-[1,1',4',1'']terphenyl-4-yl]-3H-imidazo[4,5-b]pyridine
-
belongs to a broad class of angiotensin II receptor antagonists, potent competitive inhibitor, IC50: 0.0039 mM, the acidic moiety forms direct ionic interactions with active site metal cation
5-[[(2-chlorophenyl)sulfanyl]methyl]-N-hydroxy-1,2-oxazole-3-carboxamide
P68826
-
-
5-[[(3,4-dichlorophenyl)sulfanyl]methyl]-N-hydroxy-1,2-oxazole-3-carboxamide
P68826
-
-
5-[[(3-chlorophenyl)sulfonyl]methyl]-N-hydroxy-1,2-oxazole-3-carboxamide
P68826
-
-
5-[[(4-chlorophenyl)sulfanyl]methyl]-N-hydroxy-1,2-oxazole-3-carboxamide
P68826
-
-
5-[[(4-methoxyphenyl)sulfonyl]methyl]-1,2-oxazole-3-carbohydrazide
P68826
-
-
7,8-dihydroxyflavone
-
-
8-hydroxyquinoline
-
50% inhibition at 2 mM
actinonin
-
pseudo-peptide hydroxamate, potent inhibitor Kd: 0.3 nM
actinonin
-
peptide deformylase 1 and 2
actinonin
-
50% inhibition of enzyme Def at 95 nM, 50% inhibition of enzyme form YkrB by 130 nM
actinonin
-
competitive, IC50: 43 nM
actinonin
-
IC50 against Escherichia coli peptide deformylase: 0.0000003 mM
actinonin
-
IC50: 0.000043 mM
actinonin
-
-
actinonin
Q9FUZ2, Q9FV53
AtPDF2
actinonin
-
MIC is 0.0013 mg/ml
actinonin
-
MIC is 0.034 mg/ml
actinonin
-
i.e. 3-[(1-[[2-(hydroxymethyl)-1-pyrrolidinyl]carbonyl]-2-methylpropyl)carbamoyl]octanohydroxamic acid, a naturally occurring potent PDF inhibitor
actinonin
Q9I7A8
a naturally occurring PDF inhibitor
actinonin
-
efficient inhibitor
actinonin
-
complete inhibition at 0.005 mM
actinonin
-
actinonin does exert a significant inhibitory effect on enzyme activity in the presence of 0.25 mM substrate (complete inhibition at 10 nM)
actinonin
-
slow, tight-binding inhibitor
actinonin
-
over 80% inhibition of the recombinant enzymatic activity is observed at 10 nM actinonin
aldehyde peptide
-
IC50 against Escherichia coli peptide deformylase: 0.01 mM
-
alkyl-succinate-proline hydroxamate
-
IC50 against Escherichia coli peptide deformylase: 0.000004-0.000010 mM
analogues of actinonin
-
35 different compounds were synthesized and compared to the inhibitory activity of actinonin
-
BB-83698
Staphylococcus enteridis, Staphylococcus non-hemolyticus
-
-
benzimidazole N-formyl hydroxylamine
-
-
benzothiazinone derivatives
-
IC50: 0.000005 mM
-
benzothiazolylidene hydroxamic acid derivatives
-
IC50 against Escherichia coli peptide deformylase: 0.00104 mM
-
benzyl 5-bromo-3-[2-(hydroxyamino)-2-oxoethyl]-1H-indole-1-carboxylate
-
-
benzyl 5-bromo-3-[2-(hydroxyamino)-2-oxoethyl]-1H-indole-1-carboxylate
Q819K2
-
benzyl 5-bromo-3-[2-(hydroxyamino)-2-oxoethyl]-1H-indole-1-carboxylate
-
-
beta-sulfonylhydroxamic acid
-
IC50 against Escherichia coli peptide deformylase: 0.000016 mM
carbamate N-formyl hydroxylamine
-
-
chloranil
-
-
Co(III)1
-
peptide cleaving catalyst
diprop-2-en-1-yl (2S)-2-aminobutanedioate
P68826
-
-
EDTA
-
convertion to the apoenzyme during 1 h at 10 mM
EDTA
-
slight inhibition at 10 mM
epigallocatechin 3,5-digallate
-
-
epitheaflavin monogallate
-
-
ethyl 2-amino-3-(benzylsulfanyl)propanoate
P68826
-
-
ethyl 2-amino-6-bromo-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate
P68826
-
Fe3+
-
inactivation due to oxidation of Fe2+ to Fe3+
-
formylmethionine
-
-
glutathione
-
10% inhibition at 1 mM
hematoxylin
-
-
-
heteroarylaryl(P2'/P3')N-formyl hydroxylamine
-
-
hydantoin
-
IC50 against Escherichia coli peptide deformylase: 0.0002 mM
hydrazide N-formyl hydroxylamine
-
-
imino[(5-methoxy-5-oxo-4-[2-(sulfanylmethyl)hexanoyl]aminopentyl)amino]methaneamine
-
-
imino[(5-methoxy-5-oxo-4-[2-(sulfanylmethyl)hexanoyl]aminopentyl)amino]methaneamine
-
-
isoxazole-3-hydroxamic acid derivatives
-
IC50 against Escherichia coli peptide deformylase: 0.0034 mM
-
isoxazole-3-hydroxamic acids
-
23 different compounds were synthesized and compared to the inhibitory activity of actinonin
-
LBM-415
Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus
-
-
methyl 4-(2-benzylbenzoyl)-2,5-dimethyl-1H-pyrrole-3-carboxylate
P68826
-
-
methyl-1-(sulfanylmethyl)hexanoate
-
-
methyl-1-(sulfanylmethyl)hexanoate
-
-
N-((5-bromo-2-methyl-1(phenylsulfonyl)-1H-indol-3-yl)methyl) hydroxylamine
Q9FUZ2, Q9FV53
AtPDF2
N-(1-benzenesulfonyl-5-bromo-2-methyl-1H-indol-3-ylmethyl)-N-hydroxyformamide
Q9FUZ2, Q9FV53
AtPDF2
N-(1-benzenesulfonyl-5-bromo-2-methyl-1H-indol-3-ylmethyl)-N-hydroxyformamide
-
MIC is 0.035 mg/ml
N-(1-benzenesulfonyl-5-bromo-2-methyl-1H-indol-3-ylmethyl)-N-hydroxyformamide
-
MIC is 0.065 mg/ml
N-(1-benzenesulfonyl-5-bromo-2-methyl-1H-indol-3-ylmethyl)-N-hydroxyformamide
-
-
N-(2-methoxybenzoyl)-3-methyl-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-(2-[[(2S)-2-[[1,3-bis(4-fluorophenyl)triazan-2-yl]carbonyl]-4-methylidenepyrrolidin-1-yl]carbonyl]hexyl)-N-hydroxyformamide
-
-
-
N-(2-[[(2S)-2-[[1,3-bis(4-hydroxyphenyl)triazan-2-yl]carbonyl]-4-methylidenepyrrolidin-1-yl]carbonyl]hexyl)-N-hydroxyformamide
-
-
-
N-(2-[[(2S)-2-[[1,3-bis(5-fluoro-1-oxidopyridin-2-yl)triazan-2-yl]carbonyl]-4-methylidenepyrrolidin-1-yl]carbonyl]hexyl)-N-hydroxyformamide
-
-
-
N-(2-[[(2S)-2-[[1,3-bis(5-fluoropyridin-2-yl)triazan-2-yl]carbonyl]-4-methylidenepyrrolidin-1-yl]carbonyl]hexyl)-N-hydroxyformamide
-
-
-
N-(2-[[(2S)-2-[[1,3-bis(5-methyl-1,3-thiazol-2-yl)triazan-2-yl]carbonyl]-4-methylidenepyrrolidin-1-yl]carbonyl]hexyl)-N-hydroxyformamide
-
-
-
N-(3,4-difluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-(3-chloro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-(3-chloro-4-piperidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-(3-chloro-4-pyrrolidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-(3-fluoro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-(3-fluoro-4-piperidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-(3-fluoro-4-pyrrolidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-(3-tert-butyl-2,5-dioxo-1,4-diaza-cycloheptadec-6-yl)-methyl-N-hydroxy-formamide
-
-
N-(3-tert-butyl-2,5-dioxo-1,4-diaza-cyclohexadec-6-yl)-methyl-N-hydroxy-formamide
-
-
N-(3-tert-butyl-2,5-dioxo-1,4-diaza-cycloicos-6-yl)-methyl-N-hydroxy-formamide
-
-
N-(3-tert-butyl-2,5-dioxo-1,4-diaza-cyclopentadec-6-yl)-methyl-N-hydroxy-formamide
-
-
N-(3-tert-butyl-2,5-dioxo-1,4-diaza-cyclotridec-6-yl)-methyl-N-hydroxy-formamide
-
-
N-(4-chlorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-(4-fluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-(5-fluoro-1-oxidopyridin-2-yl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
-
-
N-(benzylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-(benzyloxy)-2-(3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)acetamide
P68826
-
-
N-(cyclobutylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-(cyclobutylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-(cyclobutylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-(cyclohexylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-(cyclohexylcarbamoyl)-3-methyl-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-(cyclohexylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-(cyclohexylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-(cyclopentylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-(cyclopentylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propancoyl]-L-prolinamide
-
-
-
N-(cyclopentylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propancoyl]-L-prolinamide
-
-
-
N-(R)-[2-(hydroxyaminocarbonyl)methyl]-4-methylpentanoyl-L-naphthylalanyl-L-alanine amide
-
-
-
N-(tert-butoxycarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-(tert-butylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-acetyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-acetyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-alkyl urea hydroxamate
-
IC50 against Escherichia coli peptide deformylase: 0.000003 mM
N-benzoyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-benzoyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-benzoyl-3-methyl-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-benzyloxycarbonyl-Leu-norleucinal
-
most potent competitive inhibitor
-
N-carbamoyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-formyl-L-methionylalanine
-
inhibition above 4 mM
N-formyl-Met-Ala-Ser
-
the enzyme activity of truncated PDF-1DeltaN37 is inhibited by an excess of N-formyl-Met-Ala-Ser substrate
N-formyl-N-hydroxy-2-(3-benzoylphenoxy)ethylamine
-
i.e. SB 543668, IC50: 0.00115 mM
N-formyl-N-hydroxy-2-(3-benzoylphenoxy)ethylamine
-
i.e. SB 543668, IC50: 0.00032 mM
N-formyl-N-hydroxy-2-(3-benzoylphenoxy)ethylamine
-
i.e. SB 543668, IC50: 0.0022 mM
N-formyl-N-hydroxy-2-(3-benzoylphenoxy)ethylamine
-
i.e. SB 543668, IC50: 0.0039 mM
N-formyl-N-hydroxy-3-phenylpropylamine
-
i.e. SB 485345, IC50: 0.00016 mM
N-formyl-N-hydroxy-3-phenylpropylamine
-
i.e. SB 485345, IC50: 0.00015 mM
N-formyl-N-hydroxy-3-phenylpropylamine
-
i.e. SB 485345, IC50: 0.00025 mM
N-formyl-N-hydroxy-3-phenylpropylamine
-
i.e. SB 485345, IC50: 0.0004 mM
N-hydroxy urea
-
IC50 against Escherichia coli peptide deformylase: 0.0022 mM
N-hydroxy-2-(1H-indol-3-yl)acetamide
-
-
N-hydroxy-2-(1H-indol-3-yl)acetamide
Q819K2
-
N-hydroxy-2-(1H-indol-3-yl)acetamide
-
-
N-hydroxy-2-(2-oxo-1,4-dihydroquinazolin-3(2H)-yl)acetamide
-
IC50: 0.0029 mM
N-hydroxy-2-(5-methoxy-1H-indol-3-yl)acetamide
-
-
N-hydroxy-2-(5-methoxy-1H-indol-3-yl)acetamide
Q819K2
-
N-hydroxy-2-(5-methoxy-1H-indol-3-yl)acetamide
-
-
N-hydroxy-2-(substituted phenyl) acetamide
-
-
N-hydroxy-2-[2-oxo-5-(trifluoromethyl)-1,4-dihydroquinazolin-3(2H)-yl]acetamide
-
IC50: 0.00023 mM
N-hydroxy-5-([[2-(propan-2-yl)phenyl]sulfanyl]methyl)-1,2-oxazole-3-carboxamide
P68826
-
-
N-hydroxy-5-([[4-(trifluoromethyl)phenyl]sulfanyl]methyl)-1,2-oxazole-3-carboxamide
P68826
-
-
N-hydroxy-N-(3-hydrazino-3-oxopropyl)formamide
-
-
N-hydroxy-N-[(2R)-2-[[(2S)-2-(morpholin-4-ylcarbonyl)-2,5-dihydro-1H-pyrrol-1-yl]carbonyl]hexyl]formamide
-
-
N-hydroxy-N-[3-(6-methylpyridine-2-yl)propyl]formamide
-
i.e. SB 505684, IC50: 0.0028 mM
N-hydroxy-N-[3-(6-methylpyridine-2-yl)propyl]formamide
-
i.e. SB 505684, IC50: 0.00104 mM
N-hydroxy-N-[3-(6-methylpyridine-2-yl)propyl]formamide
-
i.e. SB 505684, IC50: 0.00109 mM
N-hydroxy-N-[3-(6-methylpyridine-2-yl)propyl]formamide
-
i.e. SB 505684, IC50: 0.0022 mM
N-methoxy-2-(3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)acetamide
P68826
-
-
N-methoxy-N-methyl-2-(3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)acetamide
P68826
-
-
N-oxo-2-[3-oxo-4-(2-phenylethyl)-3,4-dihydro-2H-1,4-benzothiazin-2-yl]acetamide
P68826
-
-
N-oxo-2-[3-oxo-4-(tetrahydro-2H-pyran-2-ylmethyl)-3,4-dihydro-2H-1,4-benzothiazin-2-yl]acetamide
P68826
-
-
N-oxo-2-[3-oxo-4-[2-oxo-2-(pyrrolidin-1-yl)ethyl]-3,4-dihydro-2H-1,4-benzothiazin-2-yl]acetamide
P68826
-
-
N-substituted methionine hydrazide
-
IC50 against Escherichia coli peptide deformylase: 0.2 mM
N-sulfonyl-L-Val-L-Leu-hydroxamate
-
-
N-trans-caffeoyltyramine
-, Q672W7
IC50: 0.0108 mM
N-[(2-aminoethyl)carbamoyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-[(2-methoxyethyl)carbamoyl]-3-methyl-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-[(2-methoxyphenyl)carbamoyl]-3-methyl-L-valyl-N-hydroxy-N2-(3-methylbutyl)glycinamide
Q9I7A8
-
N-[(2-methoxyphenyl)carbamoyl]-3-methyl-L-valyl-N2-(2-cyclopentylethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-[(2-methoxyphenyl)carbamoyl]-3-methyl-L-valyl-N2-(cyclobutylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-[(2-methoxyphenyl)carbamoyl]-3-methyl-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-[(2-methoxyphenyl)carbamoyl]-3-methyl-L-valyl-N2-(cyclopropylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-[(2-methoxyphenyl)carbamoyl]alanyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-[(2-methoxyphenyl)carbamoyl]leucyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-[(2-methoxyphenyl)carbamoyl]norleucyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-[(2-methoxyphenyl)carbamoyl]phenylalanyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-[(2-methoxyphenyl)carbamoyl]valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
-
N-[(2R)-2-(cyclopentylmethyl)-3-(2-[5-fluoro-6-[(9aS)-hexahydropyrazino[2,1-c][1,4]oxazin-8(1H)-yl]-2-methylpyrimidin-4-yl]hydrazinyl)-3-oxopropyl]-N-hydroxyformamide
-
-
-
N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide
-
-
-
N-[(2R)-2-[[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]carbonyl]heptyl]-N-hydroxyformamide
P96275
-
N-[(2R)-2-[[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]carbonyl]hexyl]-N-hydroxyformamide
P96275
-
N-[(2R)-2-[[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]carbonyl]pentyl]-N-hydroxyformamide
P96275
-
N-[(2R)-2-[[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]carbonyl]heptyl]-N-hydroxyformamide
P96275
-
N-[(2R)-2-[[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]carbonyl]hexyl]-N-hydroxyformamide
P96275
-
N-[(2R)-2-[[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]carbonyl]pentyl]-N-hydroxyformamide
P96275
-
N-[(2R)-3-[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]-2-(cyclopentylmethyl)-3-oxopropyl]-N-hydroxyformamide
P96275
-
N-[(2R)-3-[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]-2-benzyl-3-oxopropyl]-N-hydroxyformamide
P96275
-
N-[(2R)-3-[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]-2-(cyclopentylmethyl)-3-oxopropyl]-N-hydroxyformamide
P96275
-
N-[(2R)-3-[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]-2-benzyl-3-oxopropyl]-N-hydroxyformamide
P96275
-
N-[(3-amino-3-oxopropyl)carbamoyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-[(cyclobutyloxy)carbonyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-[(cyclohexyloxy)carbonyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
-
-
N-[2-(5-bromo-2-methyl-1H-indole-3-yl)methyl]hydroxylamine
Q9FUZ2, Q9FV53
; AtPDF2
N-[2-(cyclopentylmethyl)-3-[(2S)-2-[(1,3-dicyclopropyltriazan-2-yl)carbonyl]-4-methylidenepyrrolidin-1-yl]-3-oxopropyl]-N-hydroxyformamide
-
-
-
N-[2-([(2S)-2-[(1,3-diphenyltriazan-2-yl)carbonyl]-4-methylidenepyrrolidin-1-yl]carbonyl)hexyl]-N-hydroxyformamide
-
-
-
N-[3-(4-acetamidobutyl)-2,5-dioxo-1,4-diaza-cyclopentadec-6-yl]methyl-N-hydroxy-formamide
-
-
N-[3-(4-aminobutyl)-2,5-dioxo-1,4-diazacycloicos-6-yl]methyl-N-hydroxyformamide
-
-
N-[3-(4-aminobutyl)-2,5-dioxo-1,4-diazacyclopentadec-6-yl]methyl-N-hydroxyformamide
-
-
N-[3-chloro-4-(4-oxidomorpholin-4-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-[3-fluoro-4-(1-oxidopiperidin-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-[3-fluoro-4-(1H-pyrrol-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-[3-fluoro-4-(4-methyl-1,4-diazepan-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-[3-fluoro-4-(4-oxidomorpholin-4-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-[3-[(2S)-2-[[1,3-bis(4-fluorophenyl)triazan-2-yl]carbonyl]-4-methylidenepyrrolidin-1-yl]-2-(cyclopentylmethyl)-3-oxopropyl]-N-hydroxyformamide
-
-
-
N-[3-[(2S)-2-[[1,3-bis(5-fluoro-1-oxidopyridin-2-yl)triazan-2-yl]carbonyl]-4-methylidenepyrrolidin-1-yl]-2-(cyclopentylmethyl)-3-oxopropyl]-N-hydroxyformamide
-
-
-
N-[3-[(2S)-2-[[1,3-bis(5-fluoropyridin-2-yl)triazan-2-yl]carbonyl]-4-methylidenepyrrolidin-1-yl]-2-(cyclopentylmethyl)-3-oxopropyl]-N-hydroxyformamide
-
-
-
N-[3-[(2S)-2-[[1,3-bis(5-methyl-1,3-thiazol-2-yl)triazan-2-yl]carbonyl]-4-methylidenepyrrolidin-1-yl]-2-(cyclopentylmethyl)-3-oxopropyl]-N-hydroxyformamide
-
-
-
N-[4-(4-ethylpiperazin-1-yl)-3-fluorophenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-[4-(4-ethylpiperazin-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus, Streptococcus pneumoniae
-
-
N-[4-(trifluoromethyl)benzoyl]-L-Met
-
time-dependent inhibition of zinc and cobalt deformylase, inhibition of full-length Co-PDF is competitive
N-[4-(trifluoromethyl)benzoyl]-L-Met hydrazide
-
time-dependent inhibition of zinc and cobalt deformylase
N-[[(3S,6R)-3-tert-butyl-2,5-dioxo-1,4-diazacyclopentadecan-6-yl]methyl]-N-hydroxyformamide
-
Zn-BbPDF1-HT
N-[[5-bromo-2-methyl-1-(phenylsulfonyl)-1H-indol-3-yl]methyl]-N-hydroxyformamide
-
-
N-[[5-bromo-2-methyl-1-(phenylsulfonyl)-1H-indol-3-yl]methyl]-N-hydroxyformamide
Q819K2
-
N-[[5-bromo-2-methyl-1-(phenylsulfonyl)-1H-indol-3-yl]methyl]-N-hydroxyformamide
-
-
N2-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-phenyl-L-lysinamide
-
-
naphthalen-1-yl[[3-(trifluoromethyl)benzyl]amino]acetic acid
P68826
-
-
Ni2+
-
addition of 1 mM Ni2+ increases the PDF activity by 70%, with 20 mM Ni2+, the activity decreases by 23% compared with the low Ni2+ concentration
Ni2+
-
less than 40% residual activity at 0.1 mM
p-chloromercuribenzoate
-
potent inhibitor, IC50: 0.07 mM
Peptide aldehydes
-
peptide aldehydes containing a methional or leucinal
Phe-Arg-beta-naphthylamide
-
-
piperidin-4-yl [1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolyl]carbamate
-
-
-
Polyethylene glycol
-
-
polylethylene glycol
-
competitive inhibitor with respect to formylmethionine
-
pseudopeptide metabolites
-
IC50 against Escherichia coli peptide deformylase: 0.000030 mM
-
Purpurogallin
-
-
pyrogallin
-
-
pyrrolidine bicyclic(P2'/P3')compounds
-
-
-
quinalizarin
-
-
quinazoline hydroxamic acid
-
IC50 against Escherichia coli peptide deformylase: 0.00012 mM
reverse hydroxamate
-
British Biotech BB-83698
reverse hydroxamates
-
-
-
siRNA HsPDF 581-601
-
transfection of HeLa cells
-
siRNA HsPDF 659-679
-
transfection of HeLa cells
-
SKI-AC-1
-
IC50: 0.000072 mM, actionin analog
SKI-AC-10
-
IC50: 0.000078 mM, actionin analog
SKI-AC-11101
-
IC50: 0.000076 mM, actionin analog
-
SKI-AC-111101
-
IC50: 0.0000600 mM, actionin analog
SKI-AC-111110
-
IC50: 0.000427 mM, actionin analog
-
SKI-AC-111111
-
IC50: 0.000069 mM, actionin analog
SKI-AC-111112
-
IC50: 0.000234 mM, actionin analog
-
SKI-AC-111113
-
IC50: 0.000177 mM, actionin analog
-
SKI-AC-111114
-
IC50: 0.000300 mM, actionin analog
-
SKI-AC-11112
-
IC50: 0.000365 mM, actionin analog
-
SKI-AC-11113
-
IC50: 0.000500 mM, actionin analog
-
SKI-AC-11114
-
IC50: 0.000276 mM, actionin analog
-
SKI-AC-11115
-
IC50: 0.000473 mM, actionin analog
-
SKI-AC-11116
-
IC50: 0.000335 mM, actionin analog
-
SKI-AC-11117
-
IC50: 0.000086 mM, actionin analog
SKI-AC-11118
-
IC50: 0.000115 mM, actionin analog
SKI-AC-11119
-
IC50: 0.000134 mM, actionin analog
-
SKI-AC-11128
-
IC50: 0.000522 mM, actionin analog
-
SKI-AC-11138
-
IC50: 0.000500 mM, actionin analog
-
SKI-AC-11148
-
IC50: 0.0000600 mM, actionin analog
-
SKI-AC-11158
-
IC50: 0.000500 mM, actionin analog
-
SKI-AC-11168
-
IC50: 0.000192 mM, actionin analog
-
SKI-AC-11178
-
IC50: 0.000200 mM, actionin analog
-
SKI-AC-11188
-
IC50: 0.000120 mM, actionin analog
-
SKI-AC-11198
-
IC50: 0.000090 mM, actionin analog
SKI-AC-3
-
IC50: 0.000500 mM, actionin analog
-
SKI-AC-5
-
IC50: 0.000400 mM, actionin analog
-
SKI-AC-51121
-
IC50: 0.000400 mM, actionin analog
-
SKI-AC-6
-
IC50: 0.000097 mM, actionin analog
SKI-AC-8
-
IC50: 0.000093 mM, actionin analog
SKI-AC-9
-
IC50: 0.000151 mM, actionin analog
-
SKI-AC-I-18
-
IC50: 0.000404 mM, actionin analog
-
SKI-AC-I-19
-
IC50: 0.000239 mM, actionin analog
-
tert-butyl 1-[(2R)-2-[2-(hydroxyamino)-2-oxoethyl]hexanoyl]-L-prolinate
-
-
theaflavin
-
-
theaflavin monogallate
-
-
thio peptide
-
IC50 against Escherichia coli peptide deformylase: 0.000019 mM, thiol chelating group combined with the best substrate motif
-
thioacetic acid S-[3-(5-bromo-1H-indol-3-yl)-2-oxopropyl] ester
Q9FUZ2, Q9FV53
AtPDF2
thioacetic acid S-[3-(indol-3-yl)-2-oxopropyl] ester
Q9FUZ2, Q9FV53
; AtPDF2
thiol peptide
-
IC50 against Escherichia coli peptide deformylase: 0.0025 mM, designed based on the best substrate identified
-
Thiophenol
-
IC50: 0.5 mM
thyropropic acid derivatives
-
IC50 against Escherichia coli peptide deformylase: 0.001 mM
-
urea analogs(P2'/P3')of N-formyl hydroxylamine
-
-
Zn2+
-
converting enzyme to inactive state
Zn2+
-
tightly bound, very poor activity
additional information
-
the results of the study may provide the basis for the design of more potent and selective deformylase inhibitors as potential antibacterial agents
-
additional information
-
theoretical study of Escherichia coli peptide deformylase inhibition by several drugs
-
additional information
-
development of a high-throughput assay to screen for inhibitors
-
additional information
-
purified thioredoxin-fused PDF containing Fe2+ ion is inactivated during purification
-
additional information
-
not inhibited by actinonin
-
additional information
-
there is no significant inhibitory effect of actinonin on enzyme activity when the concentration of substrate is 4 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenantroline
-
1-5 mM strongly activates deformylase activity, activity increases 6fold at 1 mM
EDTA
-
1-20 mM strongly activates deformylase activity, activity increases up to 70% at 10 mM
KCl
-
2fold increase within 0-0.5 M, beyond 0.5 M smaller extend of increase
N-formyl-Met-Ala-Ser
-
the activity increases with substrate concentration up to 35 mM
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.027
-
formyl-L-Met-Leu-p-nitroanilide
-
-
8.14
-
formyl-MAHA
-
-
42.9
-
formyl-MEHQ
-
-
0.14
-
formyl-Met-Ala
-
Zn2+ PdfC
0.35
-
formyl-Met-Ala
-
Ni2+ PdC
1.5
-
formyl-Met-Ala
-
Ni2+ PdB
2.7
-
formyl-Met-Ala
-
Zn2+ PdfB
3.4
-
formyl-Met-Ala
-
mutant R79A
4.1
-
formyl-Met-Ala
-
wild-type
5
-
formyl-Met-Ala
-
mutant R79K
5.6
-
formyl-Met-Ala
-
mutant R78K
6.4
-
formyl-Met-Ala
-
mutant R78A
6.5
-
formyl-Met-Ala
-
mutant R77A
6.9
-
formyl-Met-Ala
-
mutant R77K
9.3
-
formyl-Met-Ala
-
mutant R77D
11.7
-
formyl-Met-Ala
-
mutant R78D
14
-
formyl-Met-Ala
-
Zn2+ PdfA
28
-
formyl-Met-Ala
-
Ni2+ PdA
1.7
-
formyl-Met-Ala-His-Ala
-
-
2.5
-
formyl-Met-Ala-His-Ala-Ala-Gln
-
-
0.34
-
formyl-Met-Ala-Lys-Tyr
-
Zn2+ PdB
1.3
-
formyl-Met-Ala-Lys-Tyr
-
Ni2+ PdB
1.4
-
formyl-Met-Ala-Lys-Tyr
-
Zn2+ PdfB
2.1
-
formyl-Met-Ala-Lys-Tyr
-
Ni2+ PdC
3.8
-
formyl-Met-Ala-Lys-Tyr
-
Zn2+ PdfC
6.1
-
formyl-Met-Ala-Lys-Tyr
-
Zn2+ PdfA
7.6
-
formyl-Met-Ala-Lys-Tyr
-
Ni2+ PdA
0.3
-
formyl-Met-Ala-Ser
-
Ni2+ PdB
1.7
-
formyl-Met-Ala-Ser
-, Q672W7
pH 7.5, 37C, Co2+-containing enzyme
2.4
-
formyl-Met-Ala-Ser
-
-
2.4
-
formyl-Met-Ala-Ser
-
Ni2+ PdC
2.6
-
formyl-Met-Ala-Ser
-
pH 7.6, 25C
4
-
formyl-Met-Ala-Ser
-
pH 7.6, 25C
4.8
-
formyl-Met-Ala-Ser
-
pH 7.6, 25C
9.1
-
formyl-Met-Ala-Ser
-
pH 7.6, 25C
11
-
formyl-Met-Ala-Ser
-
-
20
-
formyl-Met-Ala-Ser
-
Zn2+ PdA
21
-
formyl-Met-Ala-Ser
-
Ni2+ PdA
0.047
-
formyl-Met-Leu-p-nitroanilide
-
-
1.85
-
formyl-Met-Thr-Gln-Ser-His
-
-
0.45
-
formyl-Met-Thr-Met-His
-
-
4
-
formyl-Met-Thr-Met-His-Thr-Thr
-
-
1.351
-
formyl-MFAD
-
-
3.01
-
formyl-MFHQ
-
-
5.14
-
formyl-MGHQ
-
-
0.521
-
formyl-MLHQ
-
-
5.76
-
formyl-MLKL
-
-
4.085
-
formyl-MMYA
-
-
2.443
-
formyl-MNFA
-
-
12.44
-
formyl-MNPL
-
-
24.09
-
formyl-MPQL
-
-
2.97
-
formyl-MRHQ
-
-
1.56
-
formyl-MTHQ
-
-
2.287
-
formyl-MTHQ
-
-
2.135
-
formyl-MTMH
-
-
1.181
-
formyl-MTPM
-
-
52
-
N-formyl-Ala-Ala-Ala
-
nickel-containing enzyme
0.42
-
N-formyl-Arg(NH2)
-
enzyme form PDF1A
0.5
-
N-formyl-Arg(NH2)
-
enzyme form Ni-PDF1B
0.74
-
N-formyl-Asp(NH2)
-
enzyme form PDF1A
1
-
N-formyl-Asp(NH2)
-
enzyme form Ni-PDF1B
0.027
-
N-formyl-beta-thiaphenyl-Lys-p-nitroanilide
-
recombinant Fe2+-bound enzyme
-
0.03
-
N-formyl-beta-thiaphenyl-Lys-p-nitroanilide
-
recombinant Co2+-bound enzyme
-
0.018
-
N-formyl-beta-thiaphenyl-lysyl-p-nitroanilide
-
recombinant mutant E133D Co2+-bound enzyme
-
2.9
-
N-formyl-Gly(NH2)
-
enzyme form PDF1A
4.3
-
N-formyl-Gly(NH2)
-
enzyme form Ni-PDF1B
2.227
-
N-formyl-L-Met-L-Ala-L-Ser
Q5HPX6, Q5HQ78, -
Co2+-substituted PDF-1, in 50 mM HEPES, pH 7.5, at 37C
0.052
-
N-formyl-L-Met-L-Leu-4-nitroanilide
-
in 50 mM potassium phosphate buffer, pH 7.0, temperature not specified in the publication
-
8.8
-
N-formyl-L-Met-Val
-
-
0.79
-
N-formyl-Leu(NH2)
-
enzyme form PDF1A
4.1
-
N-formyl-Met
-
-
4.1
-
N-formyl-Met-Ala
-
-
6.2
-
N-formyl-Met-Ala
-
nickel-containing enzyme
15
-
N-formyl-Met-Ala
-
-
19
-
N-formyl-Met-Ala
-
Fe2+-bound enzyme
0.95
-
N-formyl-Met-Ala-Phe-Tyr-beta-Ala-Arg
-
-
0.25
-
N-formyl-Met-Ala-Ser
-
enzyme form PDF1A
0.27
-
N-formyl-Met-Ala-Ser
-
enzyme form PDF1A
2.3
-
N-formyl-Met-Ala-Ser
-
enzyme YkrB
2.4
-
N-formyl-Met-Ala-Ser
-
-
2.5
-
N-formyl-Met-Ala-Ser
-
mutant enzyme G151D, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
3
-
N-formyl-Met-Ala-Ser
-
enzyme Def
3.9
-
N-formyl-Met-Ala-Ser
-
nickel-containing enzyme
4.3
-
N-formyl-Met-Ala-Ser
-
wild type enzyme, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
4.4
-
N-formyl-Met-Ala-Ser
-
mutant enzyme G151A, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
5
-
N-formyl-Met-Ala-Ser
-
wild type enzyme, at pH 7.2 and 37C
5.6
-
N-formyl-Met-Ala-Ser
-
enzyme form Ni-PDF1B
7.8
-
N-formyl-Met-Ala-Ser
-
mutant enzyme G49C, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
8.2
-
N-formyl-Met-Ala-Ser
-
enzyme form PDF1B
9.2
-
N-formyl-Met-Ala-Ser
-
mutant enzyme F134C, at pH 7.2 and 37C
9.3
-
N-formyl-Met-Ala-Ser
-
mutant enzyme R137S, at pH 7.2 and 37C
22.37
-
N-formyl-Met-Ala-Ser
-
truncated PDF-1DeltaN37, in 50 mM KH2PO4, pH 7.5, at 37C
48
-
N-formyl-Met-Ala-Ser
-
mutant enzyme F134C/R137S, at pH 7.2 and 37C
8
-
N-formyl-Met-Leu-Glu
-
enzyme form PDF1A
12
-
N-formyl-Met-Leu-Glu
-
enzyme form Ni-PDF1B
2.64
-
N-formyl-Met-Leu-NH2
-
recombinant Fe2+-bound enzyme
0.23
-
N-Formyl-Met-Leu-Phe
-
enzyme form PDF1A
4
-
N-formyl-Met-Lys
-
nickel-containing enzyme
2
-
N-formyl-Met-Ser-Asn-Glu
-
enzyme form Ni-PDF1B
4.1
-
N-formyl-Met-Ser-Asn-Glu
-
enzyme form PDF1A
5.5
-
N-formyl-Met-Ser-Asn-Glu
-
nickel-containing enzyme
7.1
-
N-Formyl-Met-Val
-
truncated cobalt deformylase
8
-
N-Formyl-Met-Val
-
truncated zinc deformylase
12.4
-
N-Formyl-Met-Val
-
full-length cobalt deformylase
2.5
-
N-formyl-methionine
-
nickel-containing enzyme
1.3
-
N-formyl-Nle-Ala(NH2)
-
-
0.6
-
N-formyl-Nle-Ala-Ser
-
enzyme form PDF1A
3.5
-
N-formyl-Nle-Ala-Ser
-
nickel-containing enzyme
11
-
N-formyl-Nle-Ala-Ser
-
enzyme form Ni-PDF1B
0.66
-
N-formyl-Nle-Arg(NH2)
-
-
8.2
-
N-formyl-Nle-Asn(NH2)
-
-
4.3
-
N-formyl-Nle-Asp(NH2)
-
-
1.5
-
N-formyl-Nle-Gln(NH2)
-
-
4.3
-
N-formyl-Nle-Glu(NH2)
-
-
5.5
-
N-formyl-Nle-Gly(NH2)
-
-
1.8
-
N-formyl-Nle-Leu(NH2)
-
-
0.62
-
N-formyl-Nle-Lys(NH2)
-
-
1.7
-
N-formyl-Nle-Met(NH2)
-
-
2.9
-
N-formyl-Nle-Nle(NH2)
-
-
3
-
N-formyl-Nle-Phe(NH2)
-
-
3.7
-
N-formyl-Nle-Pro(NH2)
-
-
1
-
N-formyl-Nle-Ser(NH2)
-
-
1.8
-
N-formyl-Nle-Ser(NH2)
-
-
1.6
-
N-formyl-Nle-Thr(NH2)
-
-
1.9
-
N-formyl-Nle-Trp(NH2)
-
-
3.3
-
N-formyl-Nle-Tyr(NH2)
-
-
1.9
-
N-formyl-Nle-Val(NH2)
-
-
9.4
-
N-formyl-Nva-Ala-Ser
-
enzyme form PDF1A
3
-
N-formyl-Phe-Ala-Ser
-
enzyme form PDF1A
0.49
-
N-formyl-Phe-Tyr-Phe-His-beta-alanine-Arg
-
-
0.42
-
N-formyl-Ser(NH2)
-
enzyme form PDF1A
1
-
N-formyl-Ser(NH2)
-
enzyme form Ni-PDF1B
0.037
-
N-formyl-thiaphenyl-Ala-Leu-p-nitroanilide
-
-
0.53
-
N-formyl-thiaphenylalanyl-peptide
-
-
0.018
-
N-formylmethionyl-Leu-p-nitroanilide
-
recombinant Co2+-bound enzyme
0.02
-
N-formylmethionyl-Leu-p-nitroanilide
-
recombinant Fe2+-bound enzyme
0.02
-
N-formylmethionyl-Leu-p-nitroanilide
-
-
0.028
-
N-formylmethionyl-Leu-p-nitroanilide
-
recombinant mutant E133D Co2+-bound enzyme
7.36
-
N-formylmethionylleucyl-NH2
-
recombinant Co2+-bound enzyme
0.738
-
formyl-MVHQ
-
-
additional information
-
additional information
-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.17
-
formyl-L-Met-Leu-p-nitroanilide
-
-
0.0831
-
formyl-MAHA
-
-
0.00937
-
formyl-MEHQ
-
-
0.08
-
formyl-Met-Ala
-
mutant R79A
0.24
-
formyl-Met-Ala
-
mutant R79K
0.6
-
formyl-Met-Ala
-
mutant R77D
0.8
-
formyl-Met-Ala
-
mutant R78D
1.6
-
formyl-Met-Ala
-
mutant R77A; mutant R78K
1.7
-
formyl-Met-Ala
-
mutant R78A
1.8
-
formyl-Met-Ala
-
mutant R77K
5
-
formyl-Met-Ala
-
wild-type
0.57
-
formyl-Met-Ala-His-Ala-Ala-Gln
-
-
0.0081
-
formyl-Met-Ala-Ser
-
-
3.4
-
formyl-Met-Ala-Ser
-, Q672W7
pH 7.5, 37C, Co2+-containing enzyme
5
-
formyl-Met-Ala-Ser
-
-
103
-
formyl-Met-Ala-Ser
-
pH7.6, 25C
152
-
formyl-Met-Ala-Ser
-
pH7.6, 25C
322
-
formyl-Met-Ala-Ser
-
pH7.6, 25C
332
-
formyl-Met-Ala-Ser
-
pH7.6, 25C
0.00046
-
formyl-Met-Leu-p-nitroanilide
-
-
1.5
-
formyl-Met-Thr-Gln-Ser-His
-
-
2.2
-
formyl-Met-Thr-Met-His-Thr-Thr
-
-
0.057
-
formyl-MFAD
-
-
0.0134
-
formyl-MFHQ
-
-
0.0155
-
formyl-MGHQ
-
-
0.00275
-
formyl-MLHQ
-
-
0.0049
-
formyl-MLKL
-
-
0.0646
-
formyl-MMYA
-
-
0.0502
-
formyl-MNFA
-
-
0.0211
-
formyl-MNPL
-
-
0.0283
-
formyl-MPQL
-
-
0.0202
-
formyl-MRHQ
-
-
0.0277
-
formyl-MTHQ
-
-
0.044
-
formyl-MTHQ
-
-
0.0362
-
formyl-MTMH
-
-
0.0174
-
formyl-MTPM
-
-
0.45
-
N-formyl-1-(1-naphthyl)ethylamine
-
pH 7.2, 37C
7.1
-
N-formyl-3-amino-3-phenylpropionic acid
-
pH 7.2, 37C
5.7
-
N-formyl-Ala-Ala-Ala
-
nickel-containing enzyme
0.22
-
N-formyl-alaninol
-
pH 7.2, 37C
0.045
-
N-formyl-alpha-methyl-phenylglycine amide
-
pH 7.2, 37C
140
-
N-formyl-L-Met-L-Ala-L-Ser
Q5HPX6, Q5HQ78, -
Co2+-substituted PDF-1, in 50 mM HEPES, pH 7.5, at 37C
1370
-
N-formyl-m-methoxyphenylalanine nitrile
-
pH 7.2, 37C
232
-
N-formyl-Met-Ala
-
nickel-containing enzyme
2100
-
N-formyl-Met-Ala
-
recombinant Fe2+-bound enzyme
1030
-
N-formyl-Met-Ala-Phe-Tyr-beta-Ala-Arg
-
-
0.07
-
N-formyl-Met-Ala-Ser
-
enzyme Def
0.093
-
N-formyl-Met-Ala-Ser
-
truncated PDF-1DeltaN37, in 50 mM KH2PO4, pH 7.5, at 37C
0.17
-
N-formyl-Met-Ala-Ser
-
enzyme YkrB
2
-
N-formyl-Met-Ala-Ser
-
mutant enzyme G49C, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
3.5
-
N-formyl-Met-Ala-Ser
-
mutant enzyme G151A, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
3.6
-
N-formyl-Met-Ala-Ser
-
wild type enzyme, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
4.5
-
N-formyl-Met-Ala-Ser
-
mutant enzyme G151D, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
5
-
N-formyl-Met-Ala-Ser
-
-
6.4
-
N-formyl-Met-Ala-Ser
-
wild type enzyme, at pH 7.2 and 37C
13
-
N-formyl-Met-Ala-Ser
-
enzyme form Ni-PDF1B
15
-
N-formyl-Met-Ala-Ser
-
enzyme form PDF1A
19.6
-
N-formyl-Met-Ala-Ser
-
mutant enzyme F134C, at pH 7.2 and 37C
22
-
N-formyl-Met-Ala-Ser
-
enzyme form PDF1A
22.1
-
N-formyl-Met-Ala-Ser
-
mutant enzyme R137S, at pH 7.2 and 37C
45.3
-
N-formyl-Met-Ala-Ser
-
mutant enzyme F134C/R137S, at pH 7.2 and 37C
75
-
N-formyl-Met-Ala-Ser
-
enzyme form Ni-PDF1B
210
-
N-formyl-Met-Ala-Ser
-
nickel-containing enzyme
1.1
-
N-formyl-Met-Leu-Glu
-
enzyme form PDF1A
9.2
-
N-formyl-Met-Leu-Glu
-
enzyme form Ni-PDF1B
1280
-
N-formyl-Met-Leu-NH2
-
recombinant Co2+-bound enzyme
1320
-
N-formyl-Met-Leu-NH2
-
recombinant Fe2+-bound enzyme
19
-
N-formyl-Met-Leu-p-nitroanilide
-
recombinant Co2+-bound enzyme
38
-
N-formyl-Met-Leu-p-nitroanilide
-
-
0.7
-
N-Formyl-Met-Leu-Phe
-
enzyme form PDF1A
197
-
N-formyl-Met-Lys
-
nickel-containing enzyme
1.9
-
N-formyl-Met-Ser-Asn-Glu
-
enzyme form Ni-PDF1B
11
-
N-formyl-Met-Ser-Asn-Glu
-
enzyme form PDF1A
175
-
N-formyl-Met-Ser-Asn-Glu
-
nickel-containing enzyme
0.21
-
N-formyl-methionine
-
nickel-containing enzyme
270
-
N-formyl-Nle-Ala(NH2)
-
-
26
-
N-formyl-Nle-Ala-Ser
-
enzyme form PDF1A
136
-
N-formyl-Nle-Ala-Ser
-
enzyme form Ni-PDF1B
218
-
N-formyl-Nle-Ala-Ser
-
nickel-containing enzyme
5
-
N-formyl-Nle-Arg(NH2)
-
enzyme form PDF1A
138
-
N-formyl-Nle-Arg(NH2)
-
enzyme form Ni-PDF1B
300
-
N-formyl-Nle-Arg(NH2)
-
-
320
-
N-formyl-Nle-Asn(NH2)
-
-
0.4
-
N-formyl-Nle-Asp(NH2)
-
enzyme form PDF1A
6.4
-
N-formyl-Nle-Asp(NH2)
-
enzyme form Ni-PDF1B
120
-
N-formyl-Nle-Asp(NH2)
-
-
280
-
N-formyl-Nle-Gln(NH2)
-
-
200
-
N-formyl-Nle-Glu(NH2)
-
-
1.4
-
N-formyl-Nle-Gly(NH2)
-
enzyme form PDF1A
260
-
N-formyl-Nle-Gly(NH2)
-
-
250
-
N-formyl-Nle-His(NH2)
-
-
0.36
-
N-formyl-Nle-Leu(NH2)
-
enzyme form PDF1A
150
-
N-formyl-Nle-Leu(NH2)
-
-
230
-
N-formyl-Nle-Lys(NH2)
-
-
190
-
N-formyl-Nle-Met(NH2)
-
-
210
-
N-formyl-Nle-Nle(NH2)
-
-
200
-
N-formyl-Nle-Phe(NH2)
-
-
150
-
N-formyl-Nle-Pro(NH2)
-
-
10
-
N-formyl-Nle-Ser(NH2)
-
enzyme form PDF1A
51
-
N-formyl-Nle-Ser(NH2)
-
enzyme form Ni-PDF1B
170
-
N-formyl-Nle-Ser(NH2)
-
-
180
-
N-formyl-Nle-Thr(NH2)
-
-
130
-
N-formyl-Nle-Trp(NH2)
-
-
140
-
N-formyl-Nle-Tyr(NH2)
-
-
160
-
N-formyl-Nle-Val(NH2)
-
-
18
-
N-formyl-Nva-Ala-Ser
-
enzyme form PDF1A
0.6
-
N-formyl-Phe-Ala-Ser
-
enzyme form PDF1A
204
-
N-formyl-Phe-Tyr-Phe-His-beta-Ala-Arg
-
-
880
-
N-formyl-phenylalanine nitrile
-
pH 7.2, 37C
10.6
-
N-formyl-phenylglycine
-
pH 7.2, 37C
227
-
N-formyl-phenylglycine amide
-
pH 7.2, 37C
0.69
6.3
N-formyl-phenylglycinol
-
pH 7.2, 37C
6.08
-
N-formyl-phenylglycinol
-
pH 7.2, 37C
0.15
-
N-formyl-tert-leucine amide
-
pH 7.2, 37C
3.5
-
N-formyl-thiaphenylalanyl-peptide
-
-
26
-
N-formyl-thiaphenylalanylleucyl-p-nitroanilide
-
-
29.7
-
N-formyl-valine nitrile
-
pH 7.2, 37C
4.2
-
N-formylmethionylleucyl-p-nitroanilide
-
recombinant mutant E133D Co2+-bound enzyme
70
-
N-formylmethionylleucyl-p-nitroanilide
-
recombinant Fe2+-bound enzyme
0.00581
-
formyl-MVHQ
-
-
additional information
-
additional information
-
-
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
63
-
N-formyl-L-Met-L-Ala-L-Ser
Q5HPX6, Q5HQ78, -
Co2+-substituted PDF-1, in 50 mM HEPES, pH 7.5, at 37C
243298
0.26
-
N-formyl-Met-Ala-Ser
-
mutant enzyme G49C, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
86445
0.795
-
N-formyl-Met-Ala-Ser
-
mutant enzyme G151A, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
86445
0.842
-
N-formyl-Met-Ala-Ser
-
wild type enzyme, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
86445
0.948
-
N-formyl-Met-Ala-Ser
-
mutant enzyme F134C/R137S, at pH 7.2 and 37C
86445
1.296
-
N-formyl-Met-Ala-Ser
-
wild type enzyme, at pH 7.2 and 37C
86445
1.786
-
N-formyl-Met-Ala-Ser
-
mutant enzyme G151D, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
86445
2.143
-
N-formyl-Met-Ala-Ser
-
mutant enzyme F134C, at pH 7.2 and 37C
86445
2.387
-
N-formyl-Met-Ala-Ser
-
mutant enzyme R137S, at pH 7.2 and 37C
86445
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.052
-
(1-hydroxyaminomethyl-2-phenylethyl)carbamic acid tert-butyl ester
Q9FUZ2, Q9FV53
-
0.11
-
(1-hydroxyaminomethyl-2-phenylethyl)carbamic acid tert-butyl ester
Q9FUZ2, Q9FV53
AtPDF2
4.4e-05
-
(1-hydroxycarbamoylmethyl-2-phenylethyl)carbamic acid tert-butyl ester
Q9FUZ2, Q9FV53
-
5e-05
-
(1-hydroxycarbamoylmethyl-2-phenylethyl)carbamic acid tert-butyl ester
Q9FUZ2, Q9FV53
-
0.0003
-
(1-hydroxycarbamoylmethyl-2-phenylethyl)carbamic acid tert-butyl ester
Q9FUZ2, Q9FV53
AtPDF2
0.0004
-
(1-hydroxycarbamoylmethyl-2-phenylethyl)carbamic acid tert-butyl ester
Q9FUZ2, Q9FV53
AtPDF2
0.083
-
(1-hydroxycarbamoylmethyl-2-phenylethyl)carbamic acid tert-butyl ester
Q9FUZ2, Q9FV53
-
0.15
-
(1-hydroxycarbamoylmethyl-2-phenylethyl)carbamic acid tert-butyl ester
Q9FUZ2, Q9FV53
-
1.1e-05
-
(2R)-N-[(1S)-1-(dimethylcarbamoyl)-2,2-dimethylpropyl]-2-[[formyl(hydroxy)amino]methyl]hexanamide
-
DPPI assay
3.9e-05
-
(2R)-N-[(1S)-1-(dimethylcarbamoyl)-2,2-dimethylpropyl]-2-[[formyl(hydroxy)amino]methyl]hexanamide
-
Zn-BbPDF1-HT
7.4e-05
-
(2R)-N-[(1S)-5-amino-1-(hexylcarbamoyl)pentyl]-2-[[formyl(hydroxy)amino]methyl]heptanamide
-
DPPI assay
0.00015
-
(R)-2-[(formyl-hydroxy-amino)-methyl]-hexanoic acid ((S)-5-amino-1-phenylcarbamoyl-pentyl)-amide
-
-
4.5e-05
-
(R)-2-[(formyl-hydroxy-amino)-methyl]-hexanoic acid [(S)-5-amino-1-((R)-2-isopropoxymethyl-pyrrolidine-1-carbonyl)-pentyl]-amide
-
-
3.6e-05
-
(R)-2-[(formyl-hydroxy-amino)-methyl]-hexanoic acid [(S)-5-amino-1-((S)-2-isopropoxymethyl-pyrrolidine-1-carbonyl)-pentyl]-amide
-
-
0.17
-
1,10-phenanthroline
-
-
0.004
-
1,2-Ethanedithiol
-
-
0.0029
-
1,3-propanedithiol
-
-
0.038
-
1,5-pentanedithiol
-
-
0.25
-
1-(5-bromo-1H-indol-3-yl)-3-hydroxypropan-2-one
Q9FUZ2, Q9FV53
AtPDF2
0.56
-
1-(5-bromo-1H-indol-3-yl)-3-hydroxypropan-2-one
Q9FUZ2, Q9FV53
-
0.79
-
1-(5-bromo-1H-indol-3-yl)-3-hydroxypropan-2-one
Q9FUZ2, Q9FV53
-
0.0402
-
2,3-dimercapto-1-propanesulfonic acid
-
-
0.0109
-
2,3-Dimercapto-1-propanol
-
-
0.0001
-
2-(4-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
0.002
-
2-(4-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.7
-
2-(4-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
-
-
1.3e-05
-
2-(5-bromo-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
3.5e-05
-
2-(5-bromo-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.13
-
2-(5-bromo-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.36
-
2-(5-bromo-1H-indol-3-yl)-N-hydroxyacetamide
-
-
0.0001
-
2-(5-bromo-2-methyl-1H-indolyl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
0.003
-
2-(5-bromo-2-methyl-1H-indolyl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.45
-
2-(5-bromo-2-methyl-1H-indolyl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.45
-
2-(5-bromo-2-methyl-1H-indolyl)-N-hydroxyacetamide
-
-
1.5e-05
-
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
8e-05
-
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.25
-
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.6
-
2-(5-chloro-1H-indol-3-yl)-N-hydroxyacetamide
-
-
2e-05
-
2-(5-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
0.00016
-
2-(5-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.7
-
2-(5-fluoro-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.00033
-
2-(5-methoxy-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
0.009
-
2-(5-methoxy-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
3e-05
-
2-(6-bromo-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
0.00021
-
2-(6-bromo-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.25
-
2-(6-bromo-1H-indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.25
-
2-(6-bromo-1H-indol-3-yl)-N-hydroxyacetamide
-
-
0.0003
-
2-(indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
AtPDF2
0.0014
-
2-(indol-3-yl)-N-hydroxyacetamide
Q9FUZ2, Q9FV53
-
0.17
-
2-amino-5-mercapto-1,3,4-thiadiazole
-
in 50 mM potassium phosphate buffer, pH 7.0, temperature not specified in the publication
-
0.1
-
3-(3-acetylsulfanyl-2-oxopropyl)indole-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
AtPDF2
0.35
-
3-(3-acetylsulfanyl-2-oxopropyl)indole-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
-
0.5
-
3-(3-acetylsulfanyl-2-oxopropyl)indole-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
-
0.28
-
3-carboxymethylindol-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
AtPDF2
0.3
-
3-carboxymethylindol-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
-
0.0012
-
3-[5'-benzyl-2'-(1H-tetrazol-5-yl)-biphenyl-4-yl]-2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine
-
-
0.006
-
5-(1,1-dimethyl-propyl)-4'-(2-ethyl-5,7-dimethyl-imidazo[4,5-b]pyridin-3-yl)-biphenyl-2-sulfonylcarbamate
-
-
-
1.8e-05
-
5-bromo-3-hydroxycarbamoylmethylindole-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
AtPDF2
2.7e-05
-
5-bromo-3-hydroxycarbamoylmethylindole-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
-
0.014
-
5-bromo-3-hydroxycarbamoylmethylindole-1-carboxylic acid benzyl ester
Q9FUZ2, Q9FV53
-
1e-05
-
actinonin
Q9FUZ2, Q9FV53
;
2.7e-05
-
actinonin
Q9FUZ2, Q9FV53
-
0.00014
-
actinonin
-
at 37C, pH not specified in the publication
0.00015
-
actinonin
-
Ni2+ PdfB
0.000281
-
actinonin
-
Zn2+ PdC
0.00058
-
actinonin
-
Ni2+ PdC
0.0006
-
actinonin
-
-
0.0012
-
actinonin
-
Ni2+ PdfA
8e-06
-
BB-3497
-
-
0.00014
-
BB-3497
-
Zn2+ PdfC
0.000203
-
BB-3497
-
Ni2+ PdfB
0.00094
-
BB-3497
-
Ni2+ PdfA
0.0016
-
BB-3497
-
Ni2+ PdfC
0.04
-
methyl-1-(sulfanylmethyl)hexanoate
-
mutant enzyme I44F
0.052
-
methyl-1-(sulfanylmethyl)hexanoate
-
wild-type enzyme and mutant enzyme E88A
0.08
-
methyl-1-(sulfanylmethyl)hexanoate
-
mutant enzyme C129W
0.18
-
methyl-1-(sulfanylmethyl)hexanoate
-
mutant enzyme L125W
0.6
-
methyl-1-(sulfanylmethyl)hexanoate
-
mutant enzyme I86F
0.64
-
methyl-1-(sulfanylmethyl)hexanoate
-
mutant enzyme L125A
1.5
-
methyl-1-(sulfanylmethyl)hexanoate
-
mutant enzyme I1289S
2
-
methyl-1-(sulfanylmethyl)hexanoate
-
mutant enzyme I128R
10
-
methyl-1-(sulfanylmethyl)hexanoate
-
mutant enzyme I128F
0.0004
-
N-((5-bromo-2-methyl-1(phenylsulfonyl)-1H-indol-3-yl)methyl) hydroxylamine
Q9FUZ2, Q9FV53
AtPDF2
0.006
-
N-((5-bromo-2-methyl-1(phenylsulfonyl)-1H-indol-3-yl)methyl) hydroxylamine
Q9FUZ2, Q9FV53
-
0.5
-
N-((5-bromo-2-methyl-1(phenylsulfonyl)-1H-indol-3-yl)methyl) hydroxylamine
Q9FUZ2, Q9FV53
-
2.5e-05
-
N-(1-benzenesulfonyl-5-bromo-2-methyl-1H-indol-3-ylmethyl)-N-hydroxyformamide
Q9FUZ2, Q9FV53
AtPDF2
3e-05
-
N-(1-benzenesulfonyl-5-bromo-2-methyl-1H-indol-3-ylmethyl)-N-hydroxyformamide
Q9FUZ2, Q9FV53
-
0.035
-
N-(1-benzenesulfonyl-5-bromo-2-methyl-1H-indol-3-ylmethyl)-N-hydroxyformamide
Q9FUZ2, Q9FV53
-
0.2
-
N-(1-benzenesulfonyl-5-bromo-2-methyl-1H-indol-3-ylmethyl)-N-hydroxyformamide
-
-
7.7e-05
-
N-(3-tert-butyl-2,5-dioxo-1,4-diaza-cycloheptadec-6-yl)-methyl-N-hydroxy-formamide
-
DPPI assay
9.6e-05
-
N-(3-tert-butyl-2,5-dioxo-1,4-diaza-cyclohexadec-6-yl)-methyl-N-hydroxy-formamide
-
DPPI assay
0.0021
-
N-(3-tert-butyl-2,5-dioxo-1,4-diaza-cycloicos-6-yl)-methyl-N-hydroxy-formamide
-
DPPI assay
0.000109
-
N-(3-tert-butyl-2,5-dioxo-1,4-diaza-cyclopentadec-6-yl)-methyl-N-hydroxy-formamide
-
DPPI assay
6.3e-05
-
N-(3-tert-butyl-2,5-dioxo-1,4-diaza-cyclotridec-6-yl)-methyl-N-hydroxy-formamide
-
DPPI assay
0.0095
-
N-benzyloxycarbonyl-Leu-norleucinal
-
cobalt-substituted enzyme
-
0.0124
-
N-benzyloxycarbonyl-Leu-norleucinal
-
cobalt-substituted enzyme
-
0.026
-
N-benzyloxycarbonyl-Leu-norleucinal
-
-
-
0.0556
-
N-benzyloxycarbonyl-Leu-norleucinal
-
-
-
0.000148
-
N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide
-
mutant enzyme R137S, at pH 7.2 and 37C
-
0.00016
-
N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide
-
wild type enzyme, at pH 7.2 and 37C
-
0.001
-
N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide
-
mutant enzyme F134C/R137S, at pH 7.2 and 37C
-
143
-
N-[(2R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl]-L-tryptophan methylamide
-
mutant enzyme F134C, at pH 7.2 and 37C
-
0.001
-
N-[2-(5-bromo-2-methyl-1H-indole-3-yl)methyl]hydroxylamine
Q9FUZ2, Q9FV53
AtPDF2
0.05
-
N-[2-(5-bromo-2-methyl-1H-indole-3-yl)methyl]hydroxylamine
Q9FUZ2, Q9FV53
-
0.00071
-
N-[3-(4-acetamidobutyl)-2,5-dioxo-1,4-diaza-cyclopentadec-6-yl]methyl-N-hydroxy-formamide
-
DPPI assay
0.000258
-
N-[3-(4-aminobutyl)-2,5-dioxo-1,4-diazacycloicos-6-yl]methyl-N-hydroxyformamide
-
DPPI assay
9.2e-05
-
N-[3-(4-aminobutyl)-2,5-dioxo-1,4-diazacyclopentadec-6-yl]methyl-N-hydroxyformamide
-
DPPI assay
0.03
-
N-[4-(trifluoromethyl)benzoyl]-L-Met
-
-
0.0116
-
N-[4-(trifluoromethyl)benzoyl]-L-Met hydrazide
-
truncated Zn-PDF
0.0118
-
N-[4-(trifluoromethyl)benzoyl]-L-Met hydrazide
-
truncated Co-PDF
0.2
-
N-[4-(trifluoromethyl)benzoyl]-L-methionyl hydrazide
-
full-lenfth Co-PDF
2e-05
-
N-[[(3S,6R)-3-tert-butyl-2,5-dioxo-1,4-diazacyclopentadecan-6-yl]methyl]-N-hydroxyformamide
-
Zn-BbPDF1-HT
2.3e-05
-
N2-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-phenyl-L-lysinamide
-
DPPI assay
6
-
Polyethylene glycol
-
-
0.12
-
thioacetic acid S-[3-(5-bromo-1H-indol-3-yl)-2-oxopropyl] ester
Q9FUZ2, Q9FV53
; AtPDF2
0.17
-
thioacetic acid S-[3-(5-bromo-1H-indol-3-yl)-2-oxopropyl] ester
Q9FUZ2, Q9FV53
-
0.2
-
thioacetic acid S-[3-(indol-3-yl)-2-oxopropyl] ester
Q9FUZ2, Q9FV53
; AtPDF2
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2e-06
-
(2R)-N4-hydroxy-N1-[(2R)-1-[(2R)-2-(hydroxymethyl)pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]-2-pentylbutanediamide
P68826
pH and temperature not specified in the publication
-
4.9e-05
-
(3R)-3-[[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]carbonyl]-N-hydroxyheptanamide
P96275
-
0.000803
-
(3R)-3-[[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]carbonyl]heptanoic acid
P96275
-
1.8e-05
-
(3R)-3-[[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]carbonyl]-N-hydroxyheptanamide
P96275
-
0.000202
-
(3R)-3-[[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]carbonyl]heptanoic acid
P96275
-
0.00125
-
(E)-N-phenethyl-3-(3,4-diacetoxyphenyl)acrylamide
-, Q672W7
IC50: 0.00125 mM
0.0001
-
(RS)- and (SR)-3-[(RS)-benzenesulfinyl]heptanoic acid hydroxyamide
-
mixture of both components, IC50: 0.0001 mM, possible antimicrobial agent
3.5e-05
-
(RS)-3-(phenylsulfonyl)heptanoic acid hydroxyamide
-
IC50: 0.035 micro molar, possible antimicrobial agent
1.3e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(2,2-dimethylpropanoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1e-05
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(2,2-dimethylpropanoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.8e-05
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(2,2-dimethylpropanoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.5e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(2-methylpropanoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(2-methylpropanoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1e-05
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(2-methylpropanoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
8e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(3-methylbutanoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(3-methylbutanoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
7e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(3-methylbutanoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.2e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(cyclopropylcarbonyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.3e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(cyclopropylcarbonyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.5e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(cyclopropylcarbonyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.4e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(ethoxycarbonyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.2e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(ethoxycarbonyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
7.7e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(ethoxycarbonyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.4e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(ethylcarbamoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(ethylcarbamoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.9e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(ethylcarbamoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methoxyacetyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
8e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methoxyacetyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.3e-05
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methoxyacetyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.9e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methoxycarbonyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.5e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methoxycarbonyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.3e-05
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methoxycarbonyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.6e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methylcarbamoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.1e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methylcarbamoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
6.1e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(methylcarbamoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.1e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(prop-2-en-1-ylcarbamoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1e-05
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(prop-2-en-1-ylcarbamoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2e-05
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-(prop-2-en-1-ylcarbamoyl)-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.1e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(2-hydroxyethyl)carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.8e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(2-hydroxyethyl)carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
8.8e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(2-hydroxyethyl)carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
7.7e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(3-hydroxypropyl)carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.2e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(3-hydroxypropyl)carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
6.9e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(3-hydroxypropyl)carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.9e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(3-phenylpropyl)carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(3-phenylpropyl)carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.1e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(3-phenylpropyl)carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.6e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(cyclopentyloxy)carbonyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.4e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(cyclopentyloxy)carbonyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.1e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(cyclopentyloxy)carbonyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.8e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(propan-2-yloxy)carbonyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(propan-2-yloxy)carbonyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.7e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(propan-2-yloxy)carbonyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
6.6e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(tetrahydro-2H-pyran-4-yloxy)carbonyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.1e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(tetrahydro-2H-pyran-4-yloxy)carbonyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.4e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[(tetrahydro-2H-pyran-4-yloxy)carbonyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.5e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(1H-imidazol-4-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.3e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(1H-imidazol-4-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.3e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(1H-imidazol-4-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.1e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyrazin-2-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.4e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyrazin-2-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
6.1e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyrazin-2-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.5e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-2-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.4e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-2-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.9e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-2-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.7e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-3-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.4e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-3-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.7e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-3-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.5e-07
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-4-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.7e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-4-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.4e-06
-
1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-N-[[2-(pyridin-4-yl)ethyl]carbamoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
0.0041
-
2',2'-bisepigallocatechin monogallate
-
fluorescence polarization assay
0.002
-
2-(1-benzyl-5-chloro-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.002 mM
0.00059
-
2-(1-butyl-5-chloro-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.00059 mM
0.00076
-
2-(2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.00076 mM
1.2e-05
-
2-(4-benzyl-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)-N-oxoacetamide
P68826
pH and temperature not specified in the publication
-
5.8e-05
-
2-(4-[2-[(2,5-difluorophenyl)amino]-2-oxoethyl]-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)-N-oxoacetamide
P68826
pH and temperature not specified in the publication
-
6.9e-05
-
2-(5-bromo-1-cyclopropyl-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.000069 mM
9.8e-05
-
2-(5-bromo-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.000098 mM
4.9e-05
-
2-(5-bromo-2-oxo-1,4-dihydroquinazolin-3(2H)-yl)-N-hydroxyacetamide
-
IC50: 0.000049 mM
0.00011
-
2-(5-chloro-1-cyclopropyl-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.00011 mM
0.00012
-
2-(5-chloro-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
inhibitor shows good selectivity for peptide deformylase over several endoproteases including matrix metalloproteases, however it shows only weak antibacterial activity. IC50: 0.00012 mM
0.00031
-
2-(5-chloro-2-oxo-1,4-dihydroquinazolin-3(2H)-yl)-N-hydroxyacetamide
-
inhibitor shows good selectivity for peptide deformylase over several endoproteases including matrix metalloproteases, however it shows only weak antibacterial activity. IC50: 0.00031 mM
0.027
-
2-(5-chloro-2-oxo-1,4-dihydroquinazolin-3(2H)-yl)acetohydrazide
-
IC50: 0.027 mM
0.00087
-
2-(5-fluoro-2,2-dioxido-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl)-N-hydroxyacetamide
-
IC50: 0.00087 mM
0.00087
-
2-(5-fluoro-2-oxo-1,4-dihydroquinazolin-3(2H)-yl)-N-hydroxyacetamide
-
IC50: 0.00087 mM
0.05
-
2-(biphenyl-4-yl)-N-oxoacetamide
P68826
pH and temperature not specified in the publication
-
0.0076
-
2-butyl-5,7-dimethyl-3-[2''-(1H-tetrazol-5-ylmethyl)-[1,1',4',1'']terphenyl-4-yl]-3H-imidazo[4,5-b]pyridine
-
belongs to a class of angiotensin II receptor antagonists, potent inhibitor, IC50: 0.0076 mM
1.2e-07
-
2-phenylethanamine
-
in 50 mM potassium phosphate (pH 7.6), at 25C
8.6e-07
-
2-phenylethanamine
-
in 50 mM potassium phosphate (pH 7.6), at 25C
3.6e-06
-
2-phenylethanamine
-
in 50 mM potassium phosphate (pH 7.6), at 25C
0.00166
-
2-thioxo-4-thiazolidinone N-hexanoic acid
-
IC50 against Escherichia coli peptide deformylase: 0.00166 mM
0.00013
-
2-[2,2-dioxido-5-(trifluoromethyl)-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl]-N-hydroxyacetamide
-
IC50: 0.00013 mM
5e-05
-
2-[4-(3,3-dimethyl-2-oxobutyl)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl]-N-oxoacetamide
P68826
pH and temperature not specified in the publication
-
5e-06
-
2-[4-(3-methylbutyl)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl]-N-oxoacetamide
P68826
pH and temperature not specified in the publication
-
4.1e-05
-
2-[4-(cyclobutylmethyl)-3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl]-N-oxoacetamide
P68826
pH and temperature not specified in the publication
-
0.00029
-
2-[5-chloro-2,2-dioxido-1-(3-phenylpropyl)-1,4-dihydro-3H-2,1,3-benzothiadiazin-3-yl]-N-hydroxyacetamide
-
IC50: 0.00029 mM
0.031
-
3-methoxycatechol
-
fluorescence polarization assay
4.3e-05
-
3-methyl-N-(1,3-thiazol-2-ylcarbamoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
5.2e-05
-
3-methyl-N-(2-methylpropanoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
2.8e-05
-
3-methyl-N-(morpholin-4-ylcarbonyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
1.7e-05
-
3-methyl-N-(phenylcarbamoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
4.7e-05
-
3-methyl-N-(piperidin-1-ylcarbonyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
5.4e-05
-
3-methyl-N-(pyridin-2-ylcarbamoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
4.9e-05
-
3-methyl-N-(pyridin-3-ylcarbamoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
6.5e-05
-
3-methyl-N-(pyridin-4-ylcarbamoyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
1.9e-05
-
3-methyl-N-(thiophen-2-ylcarbonyl)-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
5e-05
-
3-methyl-N-[methyl(phenyl)carbamoyl]-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
0.0342
-
3-[5'-benzyl-2'-(1H-tetrazol-5-yl)-biphenyl-4-yl]-2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine
-
potent inhibitor, IC50: 0.0342 mM, the acidic moiety forms direct ionic interactions with active site metal cation
0.015
-
5-(1,1-dimethyl-propyl)-4'-(2-ethyl-5,7-dimethyl-imidazo[4,5-b]pyridin-3-yl)-biphenyl-2-sulfonylcarbamate
-
competitive inhibition, IC50: 0.015 mM, the acidic moiety forms direct ionic interactions with active site metal cation
-
0.0039
-
5-chloro-2-propyl-3-[2''-(1H-tetrazol-5-ylmethyl)-[1,1',4',1'']terphenyl-4-yl]-3H-imidazo[4,5-b]pyridine
-
belongs to a broad class of angiotensin II receptor antagonists, potent competitive inhibitor, IC50: 0.0039 mM, the acidic moiety forms direct ionic interactions with active site metal cation
0.0038
-
5-[[(2-chlorophenyl)sulfanyl]methyl]-N-hydroxy-1,2-oxazole-3-carboxamide
P68826
pH and temperature not specified in the publication
-
0.002
-
5-[[(3,4-dichlorophenyl)sulfanyl]methyl]-N-hydroxy-1,2-oxazole-3-carboxamide
P68826
pH and temperature not specified in the publication
-
0.0017
-
5-[[(3-chlorophenyl)sulfonyl]methyl]-N-hydroxy-1,2-oxazole-3-carboxamide
P68826
pH and temperature not specified in the publication
-
0.0023
-
5-[[(4-chlorophenyl)sulfanyl]methyl]-N-hydroxy-1,2-oxazole-3-carboxamide
P68826
pH and temperature not specified in the publication
-
0.0065
-
5-[[(4-methoxyphenyl)sulfonyl]methyl]-1,2-oxazole-3-carbohydrazide
P68826
pH and temperature not specified in the publication
-
0.017
-
7,8-dihydroxyflavone
-
fluorescence polarization assay
3e-07
-
actinonin
-
IC50 against Escherichia coli peptide deformylase: 0.0000003 mM
4.3e-05
-
actinonin
-
competitive, IC50: 43 nM
0.00012
-
actinonin
-
wild type enzyme, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
0.0008
-
actinonin
-
mutant enzyme G151D, in 20 mM phosphate buffer, pH 7.4, temperature not specified in the publication
0.0055
-
actinonin
-
fluorescence polarization assay
0.01
-
aldehyde peptide
-
IC50 against Escherichia coli peptide deformylase: 0.01 mM
-
4e-06
1e-05
alkyl-succinate-proline hydroxamate
-
IC50 against Escherichia coli peptide deformylase: 0.000004-0.000010 mM
5e-06
-
benzothiazinone derivatives
-
IC50: 0.000005 mM
-
0.00104
-
benzothiazolylidene hydroxamic acid derivatives
-
IC50 against Escherichia coli peptide deformylase: 0.00104 mM
-
1.6e-05
-
beta-sulfonylhydroxamic acid
-
IC50 against Escherichia coli peptide deformylase: 0.000016 mM
0.048
-
chloranil
-
fluorescence polarization assay
0.038
-
epigallocatechin 3,5-digallate
-
fluorescence polarization assay
0.029
-
epitheaflavin monogallate
-
fluorescence polarization assay
0.012
-
hematein
-
fluorescence polarization assay
0.0027
-
hematoxylin
-
fluorescence polarization assay
-
0.0002
-
hydantoin
-
IC50 against Escherichia coli peptide deformylase: 0.0002 mM
0.017
-
irigenol
-
fluorescence polarization assay
1.9e-07
-
N-(benzylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.7e-06
-
N-(benzylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
6.5e-06
-
N-(benzylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
0.00023
-
N-(benzyloxy)-2-(3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)acetamide
P68826
pH and temperature not specified in the publication
-
3e-07
-
N-(cyclobutylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
9e-07
-
N-(cyclobutylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.8e-06
-
N-(cyclobutylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
7e-07
-
N-(cyclobutylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5e-06
-
N-(cyclobutylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
9e-06
-
N-(cyclobutylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2e-07
-
N-(cyclohexylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
9.3e-07
-
N-(cyclohexylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4e-06
-
N-(cyclohexylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.5e-05
-
N-(cyclohexylcarbamoyl)-3-methyl-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
5e-07
-
N-(cyclohexylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.7e-06
-
N-(cyclohexylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.2e-06
-
N-(cyclohexylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1e-07
-
N-(cyclopentylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.2e-07
-
N-(cyclopentylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2e-06
-
N-(cyclopentylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.3e-07
-
N-(cyclopentylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propancoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.5e-06
-
N-(cyclopentylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propancoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
6.1e-06
-
N-(cyclopentylcarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propancoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.2e-07
-
N-(tert-butoxycarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.2e-06
-
N-(tert-butoxycarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.4e-06
-
N-(tert-butoxycarbonyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.2e-07
-
N-(tert-butylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.2e-06
-
N-(tert-butylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.4e-06
-
N-(tert-butylcarbamoyl)-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4e-06
-
N-acetyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.2e-05
-
N-acetyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.6e-05
-
N-acetyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3e-06
-
N-alkyl urea hydroxamate
-
IC50 against Escherichia coli peptide deformylase: 0.000003 mM
2.8e-07
-
N-benzoyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.8e-06
-
N-benzoyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.2e-06
-
N-benzoyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.1e-05
-
N-benzoyl-3-methyl-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
9.1e-07
-
N-carbamoyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.2e-06
-
N-carbamoyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.8e-06
-
N-carbamoyl-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
0.00032
-
N-formyl-N-hydroxy-2-(3-benzoylphenoxy)ethylamine
-
i.e. SB 543668, IC50: 0.00032 mM
0.00115
-
N-formyl-N-hydroxy-2-(3-benzoylphenoxy)ethylamine
-
i.e. SB 543668, IC50: 0.00115 mM
0.0022
-
N-formyl-N-hydroxy-2-(3-benzoylphenoxy)ethylamine
-
i.e. SB 543668, IC50: 0.0022 mM
0.00015
-
N-formyl-N-hydroxy-3-phenylpropylamine
-
i.e. SB 485345, IC50: 0.00015 mM
0.00016
-
N-formyl-N-hydroxy-3-phenylpropylamine
-
i.e. SB 485345, IC50: 0.00016 mM
0.00025
-
N-formyl-N-hydroxy-3-phenylpropylamine
-
i.e. SB 485345, IC50: 0.00025 mM
0.0022
-
N-hydroxy urea
-
IC50 against Escherichia coli peptide deformylase: 0.0022 mM
0.0029
-
N-hydroxy-2-(2-oxo-1,4-dihydroquinazolin-3(2H)-yl)acetamide
-
IC50: 0.0029 mM
0.00023
-
N-hydroxy-2-[2-oxo-5-(trifluoromethyl)-1,4-dihydroquinazolin-3(2H)-yl]acetamide
-
IC50: 0.00023 mM
0.0076
-
N-hydroxy-5-([[2-(propan-2-yl)phenyl]sulfanyl]methyl)-1,2-oxazole-3-carboxamide
P68826
pH and temperature not specified in the publication
-
0.0087
-
N-hydroxy-5-([[4-(trifluoromethyl)phenyl]sulfanyl]methyl)-1,2-oxazole-3-carboxamide
P68826
pH and temperature not specified in the publication
-
0.00104
-
N-hydroxy-N-[3-(6-methylpyridine-2-yl)propyl]formamide
-
i.e. SB 505684, IC50: 0.00104 mM
0.00109
-
N-hydroxy-N-[3-(6-methylpyridine-2-yl)propyl]formamide
-
i.e. SB 505684, IC50: 0.00109 mM
0.0028
-
N-hydroxy-N-[3-(6-methylpyridine-2-yl)propyl]formamide
-
i.e. SB 505684, IC50: 0.0028 mM
7.4e-05
-
N-methoxy-2-(3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)acetamide
P68826
pH and temperature not specified in the publication
-
0.002
-
N-methoxy-N-methyl-2-(3-oxo-3,4-dihydro-2H-1,4-benzothiazin-2-yl)acetamide
P68826
pH and temperature not specified in the publication
-
5e-06
-
N-oxo-2-[3-oxo-4-(2-phenylethyl)-3,4-dihydro-2H-1,4-benzothiazin-2-yl]acetamide
P68826
pH and temperature not specified in the publication
-
1.8e-05
-
N-oxo-2-[3-oxo-4-(tetrahydro-2H-pyran-2-ylmethyl)-3,4-dihydro-2H-1,4-benzothiazin-2-yl]acetamide
P68826
pH and temperature not specified in the publication
-
0.0004
-
N-oxo-2-[3-oxo-4-[2-oxo-2-(pyrrolidin-1-yl)ethyl]-3,4-dihydro-2H-1,4-benzothiazin-2-yl]acetamide
P68826
pH and temperature not specified in the publication
-
0.2
-
N-substituted methionine hydrazide
-
IC50 against Escherichia coli peptide deformylase: 0.2 mM
0.0108
-
N-trans-caffeoyltyramine
-, Q672W7
IC50: 0.0108 mM
3.8e-07
-
N-[(2-aminoethyl)carbamoyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.3e-06
-
N-[(2-aminoethyl)carbamoyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
7e-06
-
N-[(2-aminoethyl)carbamoyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
4.2e-05
-
N-[(2-methoxyethyl)carbamoyl]-3-methyl-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
2.1e-05
-
N-[(2-methoxyphenyl)carbamoyl]-3-methyl-L-valyl-N-hydroxy-N2-(3-methylbutyl)glycinamide
Q9I7A8
pH and temperature not specified in the publication
3.1e-05
-
N-[(2-methoxyphenyl)carbamoyl]-3-methyl-L-valyl-N2-(2-cyclopentylethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
1.3e-05
-
N-[(2-methoxyphenyl)carbamoyl]-3-methyl-L-valyl-N2-(cyclobutylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
2.9e-05
-
N-[(2-methoxyphenyl)carbamoyl]-3-methyl-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
1.5e-05
-
N-[(2-methoxyphenyl)carbamoyl]-3-methyl-L-valyl-N2-(cyclopropylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
4.2e-05
-
N-[(2-methoxyphenyl)carbamoyl]alanyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
3.2e-05
-
N-[(2-methoxyphenyl)carbamoyl]leucyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
3.6e-05
-
N-[(2-methoxyphenyl)carbamoyl]norleucyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
6.1e-05
-
N-[(2-methoxyphenyl)carbamoyl]phenylalanyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
1.3e-05
-
N-[(2-methoxyphenyl)carbamoyl]valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
1.3e-05
-
N-[(2R)-2-[[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]carbonyl]heptyl]-N-hydroxyformamide
P96275
-
1.3e-05
-
N-[(2R)-2-[[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]carbonyl]hexyl]-N-hydroxyformamide
P96275
-
2.8e-05
-
N-[(2R)-2-[[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]carbonyl]pentyl]-N-hydroxyformamide
P96275
-
1.5e-05
-
N-[(2R)-2-[[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]carbonyl]heptyl]-N-hydroxyformamide
P96275
-
1e-05
-
N-[(2R)-2-[[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]carbonyl]hexyl]-N-hydroxyformamide
P96275
-
2.4e-05
-
N-[(2R)-2-[[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]carbonyl]pentyl]-N-hydroxyformamide
P96275
-
2.1e-05
-
N-[(2R)-3-[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]-2-(cyclopentylmethyl)-3-oxopropyl]-N-hydroxyformamide
P96275
-
0.000161
-
N-[(2R)-3-[(2S)-2-(1,3-benzoxazol-2-yl)pyrrolidin-1-yl]-2-benzyl-3-oxopropyl]-N-hydroxyformamide
P96275
-
8e-06
-
N-[(2R)-3-[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]-2-(cyclopentylmethyl)-3-oxopropyl]-N-hydroxyformamide
P96275
-
6.8e-05
-
N-[(2R)-3-[(2S)-2-(1H-benzimidazol-2-yl)pyrrolidin-1-yl]-2-benzyl-3-oxopropyl]-N-hydroxyformamide
P96275
-
7.2e-07
-
N-[(3-amino-3-oxopropyl)carbamoyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.3e-06
-
N-[(3-amino-3-oxopropyl)carbamoyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
6.3e-06
-
N-[(3-amino-3-oxopropyl)carbamoyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.1e-07
-
N-[(cyclobutyloxy)carbonyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.4e-06
-
N-[(cyclobutyloxy)carbonyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.8e-06
-
N-[(cyclobutyloxy)carbonyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3e-07
-
N-[(cyclohexyloxy)carbonyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
1.5e-06
-
N-[(cyclohexyloxy)carbonyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
0.1
-
naphthalen-1-yl[[3-(trifluoromethyl)benzyl]amino]acetic acid
P68826
pH and temperature not specified in the publication
-
0.07
-
p-chloromercuribenzoate
-
potent inhibitor, IC50: 0.07 mM
1.4e-06
-
piperidin-4-yl [1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolyl]carbamate
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
2.7e-06
-
piperidin-4-yl [1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolyl]carbamate
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
5.9e-06
-
piperidin-4-yl [1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolyl]carbamate
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
3.1e-07
-
propan-2-amine
-
in 50 mM potassium phosphate (pH 7.6), at 25C
1.6e-06
-
propan-2-amine
-
in 50 mM potassium phosphate (pH 7.6), at 25C
3e-06
-
propan-2-amine
-
in 50 mM potassium phosphate (pH 7.6), at 25C
3e-05
-
pseudopeptide metabolites
-
IC50 against Escherichia coli peptide deformylase: 0.000030 mM
-
0.0088
-
Purpurogallin
-
fluorescence polarization assay
0.0057
-
pyrogallin
-
fluorescence polarization assay
0.0089
-
quinalizarin
-
fluorescence polarization assay
0.00012
-
quinazoline hydroxamic acid
-
IC50 against Escherichia coli peptide deformylase: 0.00012 mM
7.2e-05
-
SKI-AC-1
-
IC50: 0.000072 mM, actionin analog
7.8e-05
-
SKI-AC-10
-
IC50: 0.000078 mM, actionin analog
7.6e-05
-
SKI-AC-11101
-
IC50: 0.000076 mM, actionin analog
-
6e-05
-
SKI-AC-111101
-
IC50: 0.0000600 mM, actionin analog
0.000427
-
SKI-AC-111110
-
IC50: 0.000427 mM, actionin analog
-
6.9e-05
-
SKI-AC-111111
-
IC50: 0.000069 mM, actionin analog
0.000234
-
SKI-AC-111112
-
IC50: 0.000234 mM, actionin analog
-
0.000177
-
SKI-AC-111113
-
IC50: 0.000177 mM, actionin analog
-
0.0003
-
SKI-AC-111114
-
IC50: 0.000300 mM, actionin analog
-
0.000365
-
SKI-AC-11112
-
IC50: 0.000365 mM, actionin analog
-
0.0005
-
SKI-AC-11113
-
IC50: 0.000500 mM, actionin analog
-
0.000276
-
SKI-AC-11114
-
IC50: 0.000276 mM, actionin analog
-
0.000473
-
SKI-AC-11115
-
IC50: 0.000473 mM, actionin analog
-
0.000335
-
SKI-AC-11116
-
IC50: 0.000335 mM, actionin analog
-
8.6e-05
-
SKI-AC-11117
-
IC50: 0.000086 mM, actionin analog
0.000115
-
SKI-AC-11118
-
IC50: 0.000115 mM, actionin analog
0.000134
-
SKI-AC-11119
-
IC50: 0.000134 mM, actionin analog
-
0.000522
-
SKI-AC-11128
-
IC50: 0.000522 mM, actionin analog
-
0.0005
-
SKI-AC-11138
-
IC50: 0.000500 mM, actionin analog
-
6e-05
-
SKI-AC-11148
-
IC50: 0.0000600 mM, actionin analog
-
0.0005
-
SKI-AC-11158
-
IC50: 0.000500 mM, actionin analog
-
0.000192
-
SKI-AC-11168
-
IC50: 0.000192 mM, actionin analog
-
0.0002
-
SKI-AC-11178
-
IC50: 0.000200 mM, actionin analog
-
0.00012
-
SKI-AC-11188
-
IC50: 0.000120 mM, actionin analog
-
9e-05
-
SKI-AC-11198
-
IC50: 0.000090 mM, actionin analog
0.0005
-
SKI-AC-3
-
IC50: 0.000500 mM, actionin analog
-
0.0004
-
SKI-AC-5, SKI-AC-51121
-
IC50: 0.000400 mM, actionin analog
-
9.7e-05
-
SKI-AC-6
-
IC50: 0.000097 mM, actionin analog
9.3e-05
-
SKI-AC-8
-
IC50: 0.000093 mM, actionin analog
0.000151
-
SKI-AC-9
-
IC50: 0.000151 mM, actionin analog
-
0.000404
-
SKI-AC-I-18
-
IC50: 0.000404 mM, actionin analog
-
0.000239
-
SKI-AC-I-19
-
IC50: 0.000239 mM, actionin analog
-
0.0061
-
theaflavin
-
fluorescence polarization assay
0.0071
-
theaflavin monogallate
-
fluorescence polarization assay
1.9e-05
-
thio peptide
-
IC50 against Escherichia coli peptide deformylase: 0.000019 mM, thiol chelating group combined with the best substrate motif
-
0.0025
-
thiol peptide
-
IC50 against Escherichia coli peptide deformylase: 0.0025 mM, designed based on the best substrate identified
-
0.5
-
Thiophenol
-
IC50: 0.5 mM
0.043
-
thiram
-
fluorescamine assay
0.001
-
thyropropic acid derivatives
-
IC50 against Escherichia coli peptide deformylase: 0.001 mM
-
0.000116
-
1-[(2-methoxyphenyl)carbamoyl]prolyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(2-methylphenyl)-L-prolinamide
-
6.25 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(2-methylphenyl)-L-prolinamide
Staphylococcus enteridis
-
larger than 6.25 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(2-methylphenyl)-L-prolinamide
-
3.13 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(2-methylphenyl)-L-prolinamide
Staphylococcus non-hemolyticus
-
larger than 6.25 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(2-methylphenyl)-L-prolinamide
-
0.098 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(3-methylphenyl)-L-prolinamide
-
0.195 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(3-methylphenyl)-L-prolinamide
Staphylococcus enteridis
-
1.56 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(3-methylphenyl)-L-prolinamide
-
0.195 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(3-methylphenyl)-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(3-methylphenyl)-L-prolinamide
-
0.049 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methoxyphenyl)-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis
-
0.78 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methoxyphenyl)-L-prolinamide
-
0.195 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methoxyphenyl)-L-prolinamide
Staphylococcus non-hemolyticus
-
6.25 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methoxyphenyl)-L-prolinamide
-
0.049 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methylphenyl)-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis
-
0.39 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methylphenyl)-L-prolinamide
-
0.78 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methylphenyl)-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-methylphenyl)-L-prolinamide
-
0.049 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-morpholin-4-ylphenyl)-L-prolinamide
-
0.39 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-morpholin-4-ylphenyl)-L-prolinamide
Staphylococcus enteridis, Staphylococcus epidermidis
-
0.195 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-morpholin-4-ylphenyl)-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-morpholin-4-ylphenyl)-L-prolinamide
-
0.195 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-piperidin-1-ylphenyl)-L-prolinamide
-
0.39 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-piperidin-1-ylphenyl)-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-piperidin-1-ylphenyl)-L-prolinamide
-
0.195 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-pyrrolidin-1-ylphenyl)-L-prolinamide
-
0.78 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-pyrrolidin-1-ylphenyl)-L-prolinamide
Staphylococcus enteridis
-
1.56 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-pyrrolidin-1-ylphenyl)-L-prolinamide
-
0.39 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-pyrrolidin-1-ylphenyl)-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-(4-pyrrolidin-1-ylphenyl)-L-prolinamide
-
0.098 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(1H-pyrrol-1-yl)phenyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(1H-pyrrol-1-yl)phenyl]-L-prolinamide
Staphylococcus enteridis
-
0.049 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(1H-pyrrol-1-yl)phenyl]-L-prolinamide
-
0.195 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(1H-pyrrol-1-yl)phenyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
0.39 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(1H-pyrrol-1-yl)phenyl]-L-prolinamide
-
0.049 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(4-methylpiperazin-1-yl)phenyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(4-methylpiperazin-1-yl)phenyl]-L-prolinamide
Staphylococcus enteridis
-
3.13 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(4-methylpiperazin-1-yl)phenyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(4-methylpiperazin-1-yl)phenyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(4-methylpiperazin-1-yl)phenyl]-L-prolinamide
-
0.098 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(4-oxidomorpholin-4-yl)phenyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus
-
larger than 6.25 microg/ml
additional information
-
1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-N-[4-(4-oxidomorpholin-4-yl)phenyl]-L-prolinamide
-
3.13 microg/ml
0.0034
-
isoxazole-3-hydroxamic acid derivatives
-
IC50 against Escherichia coli peptide deformylase: 0.0034 mM
-
additional information
-
LBM-415
-
0.39 microg/ml
additional information
-
LBM-415
Staphylococcus enteridis
-
3.13 microg/ml
additional information
-
LBM-415
-
0.39 microg/ml
additional information
-
LBM-415
Staphylococcus non-hemolyticus
-
0.78 microg/ml
additional information
-
LBM-415
-
0.195 microg/ml
1.1e-05
-
N-(2-methoxybenzoyl)-3-methyl-L-valyl-N2-(cyclopentylmethyl)-N-hydroxyglycinamide
Q9I7A8
pH and temperature not specified in the publication
additional information
-
N-(3,4-difluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis
-
0.39 microg/ml
additional information
-
N-(3,4-difluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.195 microg/ml
additional information
-
N-(3,4-difluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
6.25 microg/ml
additional information
-
N-(3,4-difluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.195 microg/ml
additional information
-
N-(3-chloro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-(3-chloro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis, Staphylococcus epidermidis
-
0.195 microg/ml
additional information
-
N-(3-chloro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
larger than 6.25 microg/ml
additional information
-
N-(3-chloro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.195 microg/ml
additional information
-
N-(3-chloro-4-piperidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.195 microg/ml
additional information
-
N-(3-chloro-4-piperidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis
-
1.56 microg/ml
additional information
-
N-(3-chloro-4-piperidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.195 microg/ml
additional information
-
N-(3-chloro-4-piperidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
N-(3-chloro-4-piperidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.098 microg/ml
additional information
-
N-(3-chloro-4-pyrrolidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis, Staphylococcus epidermidis, Staphylococcus non-hemolyticus
-
0.195 microg/ml
additional information
-
N-(3-chloro-4-pyrrolidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.098 microg/ml
additional information
-
N-(3-fluoro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-(3-fluoro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis
-
3.13 microg/ml
additional information
-
N-(3-fluoro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-(3-fluoro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
larger than 6.25 microg/ml
additional information
-
N-(3-fluoro-4-morpholin-4-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.098 microg/ml
additional information
-
N-(3-fluoro-4-piperidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-(3-fluoro-4-piperidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
N-(3-fluoro-4-piperidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.098 microg/ml
additional information
-
N-(3-fluoro-4-pyrrolidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-(3-fluoro-4-pyrrolidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis
-
1.56 microg/ml
additional information
-
N-(3-fluoro-4-pyrrolidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-(3-fluoro-4-pyrrolidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
1.56 microg/ml
additional information
-
N-(3-fluoro-4-pyrrolidin-1-ylphenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.049 microg/ml
additional information
-
N-(4-chlorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-(4-chlorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis
-
1.56 microg/ml
additional information
-
N-(4-chlorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-(4-chlorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
N-(4-chlorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.049 microg/ml
additional information
-
N-(4-fluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-(4-fluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis
-
0.195 microg/ml
additional information
-
N-(4-fluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-(4-fluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
larger than 6.25 microg/ml
additional information
-
N-(4-fluorophenyl)-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.098 microg/ml
2.3e-06
-
N-[(cyclohexyloxy)carbonyl]-1-[(2R)-3-cyclopentyl-2-[[formyl(hydroxy)amino]methyl]propanoyl]-L-prolinamide
-
in 50 mM potassium phosphate (pH 7.6), at 25C
-
additional information
-
N-[3-chloro-4-(4-oxidomorpholin-4-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
3.13 microg/ml
additional information
-
N-[3-chloro-4-(4-oxidomorpholin-4-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis
-
1.56 microg/ml
additional information
-
N-[3-chloro-4-(4-oxidomorpholin-4-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus epidermidis, Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
N-[3-chloro-4-(4-oxidomorpholin-4-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-[3-fluoro-4-(1-oxidopiperidin-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
3.13 microg/ml
additional information
-
N-[3-fluoro-4-(1-oxidopiperidin-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis, Staphylococcus epidermidis
-
1.56 microg/ml
additional information
-
N-[3-fluoro-4-(1-oxidopiperidin-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
N-[3-fluoro-4-(1-oxidopiperidin-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.78 microg/ml
additional information
-
N-[3-fluoro-4-(1H-pyrrol-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-[3-fluoro-4-(1H-pyrrol-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis
-
0.049 microg/ml
additional information
-
N-[3-fluoro-4-(1H-pyrrol-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.195 microg/ml
additional information
-
N-[3-fluoro-4-(1H-pyrrol-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
0.39 microg/ml
additional information
-
N-[3-fluoro-4-(1H-pyrrol-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.049 microg/ml
additional information
-
N-[3-fluoro-4-(4-methyl-1,4-diazepan-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.78 microg/ml
additional information
-
N-[3-fluoro-4-(4-methyl-1,4-diazepan-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis
-
1.56 microg/ml
additional information
-
N-[3-fluoro-4-(4-methyl-1,4-diazepan-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.78 microg/ml
additional information
-
N-[3-fluoro-4-(4-methyl-1,4-diazepan-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
1.56 microg/ml
additional information
-
N-[3-fluoro-4-(4-methyl-1,4-diazepan-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-[3-fluoro-4-(4-oxidomorpholin-4-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
3.13 microg/ml
additional information
-
N-[3-fluoro-4-(4-oxidomorpholin-4-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus enteridis, Staphylococcus epidermidis
-
1.56 microg/ml
additional information
-
N-[3-fluoro-4-(4-oxidomorpholin-4-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
larger than 6.25 microg/ml
additional information
-
N-[3-fluoro-4-(4-oxidomorpholin-4-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-[4-(4-ethylpiperazin-1-yl)-3-fluorophenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis
-
1.56 microg/ml
additional information
-
N-[4-(4-ethylpiperazin-1-yl)-3-fluorophenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.39 microg/ml
additional information
-
N-[4-(4-ethylpiperazin-1-yl)-3-fluorophenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
3.13 microg/ml
additional information
-
N-[4-(4-ethylpiperazin-1-yl)-3-fluorophenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.098 microg/ml
additional information
-
N-[4-(4-ethylpiperazin-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus aureus, Staphylococcus enteridis
-
3.13 microg/ml
additional information
-
N-[4-(4-ethylpiperazin-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.78 microg/ml
additional information
-
N-[4-(4-ethylpiperazin-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
Staphylococcus non-hemolyticus
-
6.25 microg/ml
additional information
-
N-[4-(4-ethylpiperazin-1-yl)phenyl]-1-[(2R)-2-[[formyl(hydroxy)amino]methyl]hexanoyl]-L-prolinamide
-
0.195 microg/ml
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
97.2
-
-
Ni2+-bound enzyme
1200
-
-
Fe2+- and Ni2+-bound enzyme
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
7
-
formylation
6
7.2
-
-
6
-
-
for purified and modified PfPDF
7
-
-
AAP PDF inhibition assay; DPPI PDF inhibition assay
7.2
-
-
deformylation
7.4
-
-
activity assay
7.4
-
-
activity assay
7.4
-
-
activity assay
7.5
-
-, Q672W7
activity assay
7.5
-
-
activity assay
8
9
-, Q672W7
-
8.5
-
-
mutant E133D Co2+-bound enzyme
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.1
11.2
-
deformylation, nearly constant activity in this range
6.5
11
-, Q672W7
pH 6.5: about 55% of maximal activity, pH 11.0: about 90% of maximal activity
6.5
11.2
-
constant activity within
6.5
11.6
-
optimal activity within, Fe2+- and Co2+-bound enzyme
7
11
-
pH 7.0: about 70% of maximal activity, pH 11.0: about 65% of maximal activity
7.5
9.5
-
mutant E133D Co2+-bound enzyme
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
50
-
-
25
-
-
activity assay
25
-
-
room temperature, AAP PDF inhibition assay; room temperature, DPPI PDF inhibition assay
30
-
-
mPDF, activity assay
30
-
-
activity assay
37
-
-, Q672W7
activity assay
50
-
-, Q672W7
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
60
-, Q672W7
40C: about 50% of maximal activity, 60C, about 70% of maximal activity
50
80
-
50C: about 75% of maximal activity, 80C: about 70% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
AtDEF1.2 is present in both stromal and thylakoid fractions; AtDEF2 is present in both stromal and thylakoid fractions
Manually annotated by BRENDA team
B6RGY0, -, Q5VNN5
;
Manually annotated by BRENDA team
B6RGY0, -, Q5VNN5
-
Manually annotated by BRENDA team
B6RGY0, -, Q5VNN5
;
-
Manually annotated by BRENDA team
-
the enzyme interacts directly with the ribosome via its C-terminal extension. The enzyme orients its active site towards the ribosomal tunnel exit for efficient co-translational processing of emerging nascent chains. The interaction of the enzyme with the ribosome enhances cell viability
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Bacillus cereus (strain ATCC 14579 / DSM 31)
Bacillus cereus (strain ATCC 14579 / DSM 31)
Bacillus cereus (strain ATCC 14579 / DSM 31)
Ehrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas)
Ehrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas)
Enterococcus faecalis (strain ATCC 700802 / V583)
Enterococcus faecalis (strain ATCC 700802 / V583)
Escherichia coli (strain B / BL21-DE3)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli O6:K15:H31 (strain 536 / UPEC)
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1)
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
17480
-
-
-
19000
-
-
gel filtration
19200
-
-
gel filtration, electrospray ionization mass spectrometry
22000
-
-
determined by SDS-PAGE
22600
-
-
calculated molecular mass
31000
-
-
SDS-PAGE, Western blotting using an anti-his tag antibody
40000
-
-
gel filtration
43400
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-, Q672W7
x * 20970, mass spectrometry; x * 20973, calculation from nucleotide sequence
?
-
x * 22000 + X * 60000, SDS-PAGE
?
-
x * 22500, calculated from amino acid sequence; x * 29000, SDS-PAGE
?
-
x * 28000-30000, SDS-PAGE
?
-
x * 20000, SDS-PAGE
?
-
x * 25000, mature enzyme, SDS-PAGE
dimer
-
2 * 20230, mass spectrometry
dimer
Q9FV53
AtPDF1B exists as a symmetric dimer
dimer
-
-